Restriction map for
pF1KA0597
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI Aor51HI ApaI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BcnI BfrI
BlnI BlpI Bpu1102I BsaHI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FseI FspI
Hin1I HindIII KasI KpnI MfeI
MluI MluNI MscI MunI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI Sse8387I
SspBI SspI SwaI TfiI Tth111I
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 9 CGCG CG^CG
AccIII 2390 TCCGGA T^CCGGA
AhdI 1340 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 453 GTGCAC G^TGCAC
AlwNI 1639 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 453 GTGCAC G^TGCAC
Asp700I 1804 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1340 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1579 CTCGAG C^[CT]CG[GA]G
BciVI 1681 GGATAC GTATCC(6/5)
BglI 207 GCCTTCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1081 AGATCT A^GATCT
BsaAI 1496 TACGTA [CT]AC^GT[GA]
BsaBI 1381 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2390 TCCGGA [AT]^CCGG[AT]
BseAI 2390 TCCGGA T^CCGGA
BsgI 596 GTGCAG GTGCAG(16/14)
BsoBI 1579 CTCGAG C^[CT]CG[GA]G
BspEI 2390 TCCGGA T^CCGGA
BspLU11I 864 ACATGT A^CATGT
BsrDI 2316 CATTGC GCAATG(2/0)
BstUI 9 CGCG CG^CG
CfoI 2613 GCGC GCG^C
EaeI 1974 TGGCCG [CT]^GGCC[GA]
Eam1105I 1340 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2497 CCTTTACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1183 GATATC GAT^ATC
FauI 556 GCGGG CCCGC(4/6)
HaeII 2612 AGCGCC [GA]GCGC^[CT]
HhaI 2613 GCGC GCG^C
HinP1I 2613 GCGC G^CGC
HpaI 1753 GTTAAC GTT^AAC
KspI 8 CCGCGG CCGC^GG
MamI 1381 GATTTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2390 TCCGGA T^CCGGA
MvnI 9 CGCG CG^CG
NdeI 21 CATATG CA^TATG
PaeR7I 1579 CTCGAG C^TCGAG
PpuMI 2337 GGGTCCT [GA]G^G[AT]CC[CT]
SacII 8 CCGCGG CCGC^GG
SapI 808 GAAGAGC GCTCTTC(1/4)
SexAI 1341 ACCTGGT A^CC[AT]GGT
SfcI 1548 CTACAG C^T[GA][CT]AG
SmlI 1579 CTCGAG C^T[CT][GA]AG
SnaBI 1496 TACGTA TAC^GTA
SpeI 849 ACTAGT A^CTAGT
XcmI 2275 CCATGGCAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1579 CTCGAG C^TCGAG
XmnI 1804 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 864 ACATGT A^C[GA][CT]GT
969 ACACGT
AocI 2098 CCTCAGG CC^T[ACGT]AGG
2238 CCTGAGG
AvaII 795 GGACC G^G[AT]CC
2338 GGTCC
BclI 615 TGATCA T^GATCA
2147 TGATCA
BspHI 2144 TCATGA T^CATGA
2150 TCATGA
BssSI 1632 CACGAG CACGAG(-5/-1)
2662 CTCGTG
BstYI 1081 AGATCT [GA]^GATC[CT]
1692 GGATCT
Bsu36I 2098 CCTCAGG CC^T[ACGT]AGG
2238 CCTGAGG
DraI 885 TTTAAA TTT^AAA
1878 TTTAAA
DraII 1845 AGGGCCT [GA]G^G[ACGT]CC[CT]
2337 GGGTCCT
DraIII 456 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
458 CACTGTGTG
EarI 688 GAAGAG CTCTTC(1/4)
808 GAAGAG
Eco81I 2098 CCTCAGG CC^T[ACGT]AGG
2238 CCTGAGG
EcoO109I 1845 AGGGCCT [GA]G^G[ACGT]CC[CT]
2337 GGGTCCT
FbaI 615 TGATCA T^GATCA
2147 TGATCA
HapII 1679 CCGG C^CGG
2391 CCGG
HpaII 1679 CCGG C^CGG
2391 CCGG
Ksp632I 688 GAAGAG CTCTTC(1/4)
808 GAAGAG
MflI 1081 AGATCT [GA]^GATC[CT]
1692 GGATCT
MspI 1679 CCGG C^CGG
2391 CCGG
NcoI 504 CCATGG C^CATGG
2275 CCATGG
PvuII 635 CAGCTG CAG^CTG
2030 CAGCTG
RcaI 2144 TCATGA T^CATGA
2150 TCATGA
SacI 369 GAGCTC GAGCT^C
2020 GAGCTC
StuI 993 AGGCCT AGG^CCT
1806 AGGCCT
XhoII 1081 AGATCT [GA]^GATC[CT]
1692 GGATCT
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DsaI EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI HincII
HindII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SphI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I