Restriction map for
pF1KA0614
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# NO CUT ENZYMES ( 33 enzymes) #
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Acc65I AclI AgeI AhdI AscI
AseI AsnI Asp718I AspEI AvrII
BlnI BssHII CpoI DraI DrdI
Eam1105I HpaI KpnI NheI NotI
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SexAI SgrAI
SpeI SspI SwaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 7638 GTATAC GT^[AC][GT]AC
AccIII 8480 TCCGGA T^CCGGA
AflII 7119 CTTAAG C^TTAAG
BbrPI 403 CACGTG CAC^GTG
BcgI 205 GCAGCCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4215 GTATCC GTATCC(6/5)
BclI 8912 TGATCA T^GATCA
BfrI 7119 CTTAAG C^TTAAG
BseAI 8480 TCCGGA T^CCGGA
BsiWI 639 CGTACG C^GTACG
BspDI 7351 ATCGAT AT^CGAT
BspEI 8480 TCCGGA T^CCGGA
BspHI 8909 TCATGA T^CATGA
BssSI 222 CACGAG CACGAG(-5/-1)
Bst1107I 7638 GTATAC GTA^TAC
BstBI 4007 TTCGAA TT^CGAA
BstZ17I 7638 GTATAC GTA^TAC
ClaI 7351 ATCGAT AT^CGAT
EcoT22I 7347 ATGCAT ATGCA^T
FbaI 8912 TGATCA T^GATCA
FseI 8535 GGCCGGCC GGCCGG^CC
HindIII 8917 AAGCTT A^AGCTT
MluI 4943 ACGCGT A^CGCGT
MroI 8480 TCCGGA T^CCGGA
NdeI 7907 CATATG CA^TATG
NruI 2845 TCGCGA TCG^CGA
NsiI 7347 ATGCAT ATGCA^T
NspV 4007 TTCGAA TT^CGAA
PmaCI 403 CACGTG CAC^GTG
PmlI 403 CACGTG CAC^GTG
RcaI 8909 TCATGA T^CATGA
SapI 3177 GAAGAGC GCTCTTC(1/4)
ScaI 8927 AGTACT AGT^ACT
SfuI 4007 TTCGAA TT^CGAA
SnaBI 5499 TACGTA TAC^GTA
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# TWO CUT ENZYMES ( 30 enzymes) #
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AatII 3331 GACGTC GACGT^C
8152 GACGTC
Aor51HI 2920 AGCGCT AGC^GCT
3481 AGCGCT
ApaI 5916 GGGCCC GGGCC^C
8705 GGGCCC
AspI 924 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5017 GACAGGGTC
BbvCI 5588 CCTCAGC CCTCAGC(-5/-2)
2754 GCTGAGG
BglII 4682 AGATCT A^GATCT
6038 AGATCT
BsaAI 403 CACGTG [CT]AC^GT[GA]
5499 TACGTA
BsaWI 3040 ACCGGA [AT]^CCGG[AT]
8480 TCCGGA
BsmI 7725 GAATGC GAATGC(1/-1)
2135 GCATTC
BspLU11I 718 ACATGT A^CATGT
4226 ACATGT
Eco47III 2920 AGCGCT AGC^GCT
3481 AGCGCT
HincII 4753 GTCAAC GT[CT]^[GA]AC
6630 GTCAAC
HindII 4753 GTCAAC GT[CT]^[GA]AC
6630 GTCAAC
KspI 4936 CCGCGG CCGC^GG
7493 CCGCGG
MfeI 2113 CAATTG C^AATTG
3995 CAATTG
MunI 2113 CAATTG C^AATTG
3995 CAATTG
PaeR7I 6292 CTCGAG C^TCGAG
8629 CTCGAG
PflFI 924 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5017 GACAGGGTC
PshAI 678 GACGATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5184 GACATCCGTC
SacII 4936 CCGCGG CCGC^GG
7493 CCGCGG
SbfI 5196 CCTGCAGG CCTGCA^GG
8229 CCTGCAGG
SfiI 969 GGCCACCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
8526 GGCCGTGCGGGCC
SmaI 2939 CCCGGG CCC^GGG
4774 CCCGGG
SphI 2602 GCATGC GCATG^C
7345 GCATGC
Sse8387I 5196 CCTGCAGG CCTGCA^GG
8229 CCTGCAGG
Tth111I 924 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5017 GACAGGGTC
XbaI 2089 TCTAGA T^CTAGA
2404 TCTAGA
XhoI 6292 CTCGAG C^TCGAG
8629 CTCGAG
XmaCI 2939 CCCGGG C^CCGGG
4774 CCCGGG
XmaI 2939 CCCGGG C^CCGGG
4774 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 160 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI Asp700I AspHI
AvaI AvaII AviII BalI BamHI
BanI BanII BbeI BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI