Restriction map for
pF1KA0623
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# NO CUT ENZYMES ( 94 enzymes) #
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AatII AccIII AclI AcyI AgeI
AhdI AlwNI Aor51HI AscI AspEI
AspI AvaI AvrII BalI BbeI
BbrPI BcgI BciVI BclI BlnI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmI BsoBI BspEI BspHI BsrFI
BssHII Bst1107I BstEII BstPI BstZ17I
Cfr10I CpoI DrdI Eam1105I Eco47III
EcoO65I EcoRV EcoT22I FbaI FseI
Hin1I HindIII HpaI KasI KspI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SexAI SfiI SfoI SgrAI SmaI
SnaBI SphI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 1444 GGTACC G^GTACC
AccI 2048 GTCGAC GT^[AC][GT]AC
AccII 2296 CGCG CG^CG
AflII 663 CTTAAG C^TTAAG
AflIII 2222 ACATGT A^C[GA][CT]GT
Alw44I 2559 GTGCAC G^TGCAC
ApaI 2254 GGGCCC GGGCC^C
ApaLI 2559 GTGCAC G^TGCAC
AseI 121 ATTAAT AT^TAAT
AsnI 121 ATTAAT AT^TAAT
Asp718I 1444 GGTACC G^GTACC
AspHI 2559 GTGCAC G[AT]GC[AT]^C
AviII 2801 TGCGCA TGC^GCA
BamHI 526 GGATCC G^GATCC
BbvCI 1687 CCTCAGC CCTCAGC(-5/-2)
BfrI 663 CTTAAG C^TTAAG
BlpI 2622 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2622 GCTGAGC GC^T[ACGT]AGC
BsaAI 2374 TACGTG [CT]AC^GT[GA]
BsgI 364 CTGCAC GTGCAG(16/14)
BsiEI 1342 CGGCCG CG[GA][CT]^CG
BsiHKAI 2559 GTGCAC G[AT]GC[AT]^C
BsmBI 2446 GAGACG CGTCTC(1/5)
BspDI 2828 ATCGAT AT^CGAT
BspLU11I 2222 ACATGT A^CATGT
BspMI 2140 GCAGGT ACCTGC(4/8)
BsrBI 2458 GAGCGG CCGCTC(-3/-3)
BssSI 40 CTCGTG CACGAG(-5/-1)
BstAPI 2131 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2231 TTCGAA TT^CGAA
BstUI 2296 CGCG CG^CG
CelII 2622 GCTGAGC GC^T[ACGT]AGC
ClaI 2828 ATCGAT AT^CGAT
EagI 1342 CGGCCG C^GGCCG
EclXI 1342 CGGCCG C^GGCCG
Eco52I 1342 CGGCCG C^GGCCG
EcoRI 1138 GAATTC G^AATTC
FspI 2801 TGCGCA TGC^GCA
HgaI 2448 GACGC GACGC(5/10)
HincII 2048 GTCGAC GT[CT]^[GA]AC
HindII 2048 GTCGAC GT[CT]^[GA]AC
KpnI 1444 GGTACC GGTAC^C
MvnI 2296 CGCG CG^CG
NcoI 1328 CCATGG C^CATGG
NspI 2222 ACATGT [GA]CATG^[CT]
NspV 2231 TTCGAA TT^CGAA
PshBI 121 ATTAAT AT^TAAT
SalI 2048 GTCGAC G^TCGAC
SapI 2333 GCTCTTC GCTCTTC(1/4)
SbfI 2137 CCTGCAGG CCTGCA^GG
ScaI 1018 AGTACT AGT^ACT
SfuI 2231 TTCGAA TT^CGAA
SpeI 1021 ACTAGT A^CTAGT
Sse8387I 2137 CCTGCAGG CCTGCA^GG
SspI 199 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 14 enzymes) #
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AcsI 1138 GAATTC [GA]^AATT[CT]
2785 AAATTC
ApoI 1138 GAATTC [GA]^AATT[CT]
2785 AAATTC
Asp700I 2826 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2911 GAAATGGTTC
BglI 1325 GCCCCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1692 GCCCAGTCGGC
BsaI 272 GAGACC GGTCTC(1/5)
2183 GGTCTC
BsrDI 1930 CATTGC GCAATG(2/0)
263 GCAATG
BstXI 1322 CCAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1727 CCAAGCACTTGG
DraI 1768 TTTAAA TTT^AAA
2073 TTTAAA
EaeI 1342 CGGCCG [CT]^GGCC[GA]
2292 TGGCCG
EcoNI 2134 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2317 CCTGGCCCAGG
HaeII 1582 AGCGCC [GA]GCGC^[CT]
3081 GGCGCT
MaeII 1125 ACGT A^CGT
2375 ACGT
StuI 1417 AGGCCT AGG^CCT
3000 AGGCCT
XmnI 2826 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2911 GAAATGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII