Restriction map for
pF1KA0633
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# NO CUT ENZYMES ( 84 enzymes) #
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Acc65I AccIII AclI AhdI Aor51HI
AscI Asp718I AspEI AviII BamHI
BciVI BclI BglII BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI Bst1107I BstEII BstPI
BstYI BstZ17I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MfeI MflI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PmeI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccI 2883 GTCTAC GT^[AC][GT]AC
AflII 2101 CTTAAG C^TTAAG
AflIII 665 ACGCGT A^C[GA][CT]GT
AgeI 2007 ACCGGT A^CCGGT
Alw44I 97 GTGCAC G^TGCAC
ApaI 128 GGGCCC GGGCC^C
ApaLI 97 GTGCAC G^TGCAC
AseI 1449 ATTAAT AT^TAAT
AsnI 1449 ATTAAT AT^TAAT
BalI 3250 TGGCCA TGG^CCA
BfrI 2101 CTTAAG C^TTAAG
BsaBI 1861 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1248 CGTCTC CGTCTC(1/5)
BsrBI 3735 CCGCTC CCGCTC(-3/-3)
BstBI 1905 TTCGAA TT^CGAA
EcoRI 1998 GAATTC G^AATTC
KspI 3471 CCGCGG CCGC^GG
MamI 1861 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 665 ACGCGT A^CGCGT
MluNI 3250 TGGCCA TGG^CCA
MscI 3250 TGGCCA TGG^CCA
NspI 93 GCATGT [GA]CATG^[CT]
NspV 1905 TTCGAA TT^CGAA
PinAI 2007 ACCGGT A^CCGGT
PshBI 1449 ATTAAT AT^TAAT
PvuII 1185 CAGCTG CAG^CTG
SacII 3471 CCGCGG CCGC^GG
SfuI 1905 TTCGAA TT^CGAA
SspI 535 AATATT AAT^ATT
StuI 3089 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 36 enzymes) #
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AatII 190 GACGTC GACGT^C
1816 GACGTC
AfaI 1789 GTAC GT^AC
3053 GTAC
Asp700I 406 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3276 GAAAAAATTC
AspI 1170 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1243 GACATCGTC
AvaI 133 CTCGGG C^[CT]CG[GA]G
646 CTCGGG
AvrII 2484 CCTAGG C^CTAGG
2848 CCTAGG
BbrPI 580 CACGTG CAC^GTG
2527 CACGTG
BbvCI 3690 CCTCAGC CCTCAGC(-5/-2)
171 GCTGAGG
BcgI 1602 CGACACAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3444 CGAACGATCTGC
BglI 16 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
207 GCCTAGCGGGC
BlnI 2484 CCTAGG C^CTAGG
2848 CCTAGG
BlpI 1750 GCTGAGC GC^T[ACGT]AGC
3020 GCTCAGC
Bpu1102I 1750 GCTGAGC GC^T[ACGT]AGC
3020 GCTCAGC
BsaAI 580 CACGTG [CT]AC^GT[GA]
2527 CACGTG
BsaI 1168 GAGACC GGTCTC(1/5)
3300 GAGACC
BsaWI 2007 ACCGGT [AT]^CCGG[AT]
2182 ACCGGA
BsiEI 35 CGACCG CG[GA][CT]^CG
1208 CGACCG
BsoBI 133 CTCGGG C^[CT]CG[GA]G
646 CTCGGG
BsrFI 2007 ACCGGT [GA]^CCGG[CT]
2233 ACCGGC
BssHII 12 GCGCGC G^CGCGC
3123 GCGCGC
BssSI 56 CTCGTG CACGAG(-5/-1)
3778 CTCGTG
CelII 1750 GCTGAGC GC^T[ACGT]AGC
3020 GCTCAGC
Cfr10I 2007 ACCGGT [GA]^CCGG[CT]
2233 ACCGGC
EaeI 23 CGGCCA [CT]^GGCC[GA]
3250 TGGCCA
PflFI 1170 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1243 GACATCGTC
PflMI 67 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2595 CCAGTATGTGG
PmaCI 580 CACGTG CAC^GTG
2527 CACGTG
PmlI 580 CACGTG CAC^GTG
2527 CACGTG
PshAI 1345 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3589 GACCTTGGTC
RsaI 1789 GTAC GT^AC
3053 GTAC
SapI 1083 GAAGAGC GCTCTTC(1/4)
1359 GAAGAGC
TaqI 1700 TCGA T^CGA
1906 TCGA
Tth111I 1170 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1243 GACATCGTC
TthHB8I 1700 TCGA T^CGA
1906 TCGA
Van91I 67 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2595 CCAGTATGTGG
XmnI 406 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3276 GAAAAAATTC
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# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI