Restriction map for
pF1KA0674
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI BamHI BbeI BbrPI BclI
BfrI BsaAI BsaBI BseAI BsiEI
BsiWI Bsp1407I BspEI BspLU11I BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FbaI FseI HaeII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
PacI PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Van91I XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 913 CGCG CG^CG
AcyI 3593 GACGCC G[GA]^CG[CT]C
AgeI 3394 ACCGGT A^CCGGT
ApaI 3115 GGGCCC GGGCC^C
Asp700I 2858 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1212 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3144 CCTAGG C^CTAGG
BalI 96 TGGCCA TGG^CCA
BciVI 3154 GTATCC GTATCC(6/5)
BcnI 1535 CCCGG CC^[GC]GG
BglII 2040 AGATCT A^GATCT
BlnI 3144 CCTAGG C^CTAGG
BsaHI 3593 GACGCC G[GA]^CG[CT]C
BsaI 1953 GGTCTC GGTCTC(1/5)
BsgI 2068 GTGCAG GTGCAG(16/14)
BsmBI 929 CGTCTC CGTCTC(1/5)
BsmI 731 GAATGC GAATGC(1/-1)
BspDI 248 ATCGAT AT^CGAT
BsrDI 504 CATTGC GCAATG(2/0)
BstBI 1556 TTCGAA TT^CGAA
BstUI 913 CGCG CG^CG
ClaI 248 ATCGAT AT^CGAT
DraI 3514 TTTAAA TTT^AAA
DrdI 2343 GACTCGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 242 ATGCAT ATGCA^T
HgaI 3593 GACGC GACGC(5/10)
Hin1I 3593 GACGCC G[GA]^CG[CT]C
HincII 347 GTCAAC GT[CT]^[GA]AC
HindII 347 GTCAAC GT[CT]^[GA]AC
MluNI 96 TGGCCA TGG^CCA
MscI 96 TGGCCA TGG^CCA
MvnI 913 CGCG CG^CG
NaeI 1239 GCCGGC GCC^GGC
NciI 1535 CCCGG CC^[GC]GG
NgoMIV 1239 GCCGGC G^CCGGC
NsiI 242 ATGCAT ATGCA^T
NspV 1556 TTCGAA TT^CGAA
PaeR7I 2345 CTCGAG C^TCGAG
PflFI 1212 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3394 ACCGGT A^CCGGT
SacI 2086 GAGCTC GAGCT^C
SexAI 2675 ACCAGGT A^CC[AT]GGT
SfuI 1556 TTCGAA TT^CGAA
SphI 1896 GCATGC GCATG^C
Tth111I 1212 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2345 CTCGAG C^TCGAG
XmnI 2858 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 2345 CTCGAG C^[CT]CG[GA]G
3304 CCCGAG
AviII 1947 TGCGCA TGC^GCA
2482 TGCGCA
BbsI 2858 GAAGAC GAAGAC(2/6)
3571 GAAGAC
BlpI 2395 GCTGAGC GC^T[ACGT]AGC
3205 GCTAAGC
Bpu1102I 2395 GCTGAGC GC^T[ACGT]AGC
3205 GCTAAGC
BpuAI 2858 GAAGAC GAAGAC(2/6)
3571 GAAGAC
BsaWI 2141 ACCGGA [AT]^CCGG[AT]
3394 ACCGGT
BsoBI 2345 CTCGAG C^[CT]CG[GA]G
3304 CCCGAG
BstAPI 235 GCAGTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1306 GCACCTTCTGC
BstYI 1739 AGATCC [GA]^GATC[CT]
2040 AGATCT
CelII 2395 GCTGAGC GC^T[ACGT]AGC
3205 GCTAAGC
CfoI 1948 GCGC GCG^C
2483 GCGC
EcoNI 139 CCTAAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2172 CCTGGAGGAGG
FspI 1947 TGCGCA TGC^GCA
2482 TGCGCA
HhaI 1948 GCGC GCG^C
2483 GCGC
HinP1I 1948 GCGC G^CGC
2483 GCGC
MflI 1739 AGATCC [GA]^GATC[CT]
2040 AGATCT
NspI 1658 GCATGT [GA]CATG^[CT]
1896 GCATGC
PpuMI 551 AGGACCT [GA]G^G[AT]CC[CT]
3314 GGGACCC
SapI 2845 GAAGAGC GCTCTTC(1/4)
1015 GCTCTTC
XhoII 1739 AGATCC [GA]^GATC[CT]
2040 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BanI BanII BbvCI BbvI BcgI
BfaI BglI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstXI Bsu36I Cac8I Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I