Restriction map for
pF1KA0739
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI Asp718I AspEI
AspI AvaI AvrII BanII BbeI
BbrPI BclI BfrI BglI BlnI
BsaAI BsaBI BsaHI BseAI BsgI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstPI Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FbaI FseI HaeII
Hin1I HpaI KasI KpnI KspI
MamI MluI MroI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 2383 CGCG CG^CG
AflIII 2637 ACATGT A^C[GA][CT]GT
Alw44I 2935 GTGCAC G^TGCAC
ApaLI 2935 GTGCAC G^TGCAC
Asp700I 721 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2927 TGCGCA TGC^GCA
BamHI 1284 GGATCC G^GATCC
BanI 391 GGCACC G^G[CT][GA]CC
BbsI 2758 GTCTTC GAAGAC(2/6)
BbvCI 796 GCTGAGG CCTCAGC(-5/-2)
BcgI 255 GCAGAGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 64 GGATAC GTATCC(6/5)
BcnI 51 CCGGG CC^[GC]GG
BglII 446 AGATCT A^GATCT
BlpI 3081 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3081 GCTGAGC GC^T[ACGT]AGC
BpuAI 2758 GTCTTC GAAGAC(2/6)
BsaWI 293 ACCGGA [AT]^CCGG[AT]
BsmBI 297 GAGACG CGTCTC(1/5)
BspLU11I 2637 ACATGT A^CATGT
BsrDI 566 CATTGC GCAATG(2/0)
BsrFI 251 GCCGGC [GA]^CCGG[CT]
Bst1107I 3184 GTATAC GTA^TAC
BstAPI 1816 GCAACTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2383 CGCG CG^CG
BstZ17I 3184 GTATAC GTA^TAC
CelII 3081 GCTGAGC GC^T[ACGT]AGC
Cfr10I 251 GCCGGC [GA]^CCGG[CT]
EcoRI 2843 GAATTC G^AATTC
FspI 2927 TGCGCA TGC^GCA
HgaI 1598 GACGC GACGC(5/10)
HincII 2958 GTTGAC GT[CT]^[GA]AC
HindII 2958 GTTGAC GT[CT]^[GA]AC
HindIII 2341 AAGCTT A^AGCTT
MfeI 1610 CAATTG C^AATTG
MunI 1610 CAATTG C^AATTG
MvnI 2383 CGCG CG^CG
NaeI 251 GCCGGC GCC^GGC
NciI 51 CCGGG CC^[GC]GG
NgoMIV 251 GCCGGC G^CCGGC
PflMI 2411 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 2327 GAGTC GAGTC(4/5)
PstI 1986 CTGCAG CTGCA^G
SexAI 1253 ACCAGGT A^CC[AT]GGT
SphI 627 GCATGC GCATG^C
Van91I 2411 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2097 CCAGGAGATGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 721 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 937 GTAGAC GT^[AC][GT]AC
3184 GTATAC
AseI 601 ATTAAT AT^TAAT
2395 ATTAAT
AsnI 601 ATTAAT AT^TAAT
2395 ATTAAT
AspHI 1264 GTGCTC G[AT]GC[AT]^C
2935 GTGCAC
BmyI 1264 GTGCTC G[AGT]GC[ACT]^C
2935 GTGCAC
BsaI 56 GAGACC GGTCTC(1/5)
1933 GAGACC
BseRI 38 CTCCTC GAGGAG(10/8)
857 CTCCTC
BsiHKAI 1264 GTGCTC G[AT]GC[AT]^C
2935 GTGCAC
BsmI 2092 GAATGC GAATGC(1/-1)
2665 GAATGC
Bsp1286I 1264 GTGCTC G[AGT]GC[ACT]^C
2935 GTGCAC
BspMI 2909 ACCTGC ACCTGC(4/8)
1988 GCAGGT
CfoI 1419 GCGC GCG^C
2928 GCGC
DraII 1466 AGGCCCC [GA]G^G[ACGT]CC[CT]
3193 GGGGCCT
DsaI 1157 CCATGG C^C[GA][CT]GG
2261 CCATGG
EcoNI 1031 CCTTTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1248 CCTAGACCAGG
EcoO109I 1466 AGGCCCC [GA]G^G[ACGT]CC[CT]
3193 GGGGCCT
EcoT22I 625 ATGCAT ATGCA^T
2104 ATGCAT
HhaI 1419 GCGC GCG^C
2928 GCGC
HinP1I 1419 GCGC G^CGC
2928 GCGC
MaeII 1193 ACGT A^CGT
2221 ACGT
NcoI 1157 CCATGG C^CATGG
2261 CCATGG
NsiI 625 ATGCAT ATGCA^T
2104 ATGCAT
PshBI 601 ATTAAT AT^TAAT
2395 ATTAAT
SfcI 1986 CTGCAG C^T[GA][CT]AG
2836 CTACAG
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BalI BbvI BfaI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BspHI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PvuII
RcaI RsaI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII