Restriction map for
pF1KA0751
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspI AviII AvrII BanI
BbeI BbrPI BbvCI BfrI BglI
BlnI BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrFI
BssHII Bst1107I BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI EagI
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT22I FseI FspI HaeII HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PstI PvuI RsrII SacI SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
Tth111I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 2952 GTCTAC GT^[AC][GT]AC
AccII 1610 CGCG CG^CG
AclI 2838 AACGTT AA^CGTT
AcyI 3077 GACGCC G[GA]^CG[CT]C
AflIII 1210 ACGTGT A^C[GA][CT]GT
AhdI 2519 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3082 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 3164 GGGCCC GGGCC^C
Asp700I 3509 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2519 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1146 TGGCCA TGG^CCA
BamHI 485 GGATCC G^GATCC
BcgI 1087 GCAGTTTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 210 GGATAC GTATCC(6/5)
BclI 288 TGATCA T^GATCA
BcnI 3162 CCGGG CC^[GC]GG
BlpI 3064 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3064 GCTCAGC GC^T[ACGT]AGC
BsaAI 1209 TACGTG [CT]AC^GT[GA]
BsaHI 3077 GACGCC G[GA]^CG[CT]C
BsaWI 2485 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1535 TGTACA T^GTACA
BspHI 2678 TCATGA T^CATGA
BsrGI 1535 TGTACA T^GTACA
BssSI 2027 CTCGTG CACGAG(-5/-1)
BstAPI 3091 GCAACACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3316 TTCGAA TT^CGAA
BstUI 1610 CGCG CG^CG
CelII 3064 GCTCAGC GC^T[ACGT]AGC
DraII 2470 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 2420 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2294 GACATGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2519 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 2470 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 2050 GAATTC G^AATTC
FbaI 288 TGATCA T^GATCA
Hin1I 3077 GACGCC G[GA]^CG[CT]C
MluNI 1146 TGGCCA TGG^CCA
MscI 1146 TGGCCA TGG^CCA
MspA1I 3142 CAGCTG C[AC]G^C[GT]G
MvnI 1610 CGCG CG^CG
NciI 3162 CCGGG CC^[GC]GG
NspI 1629 ACATGC [GA]CATG^[CT]
NspV 3316 TTCGAA TT^CGAA
PpuMI 2470 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 2838 AACGTT AA^CGTT
PvuII 3142 CAGCTG CAG^CTG
RcaI 2678 TCATGA T^CATGA
SapI 3509 GAAGAGC GCTCTTC(1/4)
ScaI 2782 AGTACT AGT^ACT
SfaNI 2688 GATGC GCATC(5/9)
SfuI 3316 TTCGAA TT^CGAA
SpeI 1075 ACTAGT A^CTAGT
SspBI 1535 TGTACA T^GTACA
SspI 188 AATATT AAT^ATT
XbaI 1548 TCTAGA T^CTAGA
XmnI 3509 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AseI 1794 ATTAAT AT^TAAT
2697 ATTAAT
AsnI 1794 ATTAAT AT^TAAT
2697 ATTAAT
AspHI 506 GAGCAC G[AT]GC[AT]^C
2874 GAGCAC
BanII 2196 GAGCCC G[GA]GC[CT]^C
3164 GGGCCC
BsiHKAI 506 GAGCAC G[AT]GC[AT]^C
2874 GAGCAC
BsmI 1997 GAATGC GAATGC(1/-1)
330 GCATTC
BspMI 2727 ACCTGC ACCTGC(4/8)
3096 ACCTGC
BsrBI 2643 CCGCTC CCGCTC(-3/-3)
2629 GAGCGG
BstEII 1786 GGTCACC G^GT[ACGT]ACC
2417 GGTCACC
BstPI 1786 GGTCACC G^GT[ACGT]ACC
2417 GGTCACC
CfoI 18 GCGC GCG^C
945 GCGC
EcoNI 1227 CCTTAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2732 CCTTATCGAGG
EcoO65I 1786 GGTCACC G^GT[ACGT]ACC
2417 GGTCACC
FauI 2474 CCCGC CCCGC(4/6)
2642 CCCGC
HapII 2486 CCGG C^CGG
3162 CCGG
HhaI 18 GCGC GCG^C
945 GCGC
HinP1I 18 GCGC G^CGC
945 GCGC
HpaII 2486 CCGG C^CGG
3162 CCGG
MslI 46 CATTTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
526 CATAGTGATG
MspI 2486 CCGG C^CGG
3162 CCGG
NcoI 632 CCATGG C^CATGG
2187 CCATGG
PaeR7I 668 CTCGAG C^TCGAG
1346 CTCGAG
PflMI 1149 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3081 CCAGACTCTGG
PshBI 1794 ATTAAT AT^TAAT
2697 ATTAAT
StuI 1591 AGGCCT AGG^CCT
2901 AGGCCT
Van91I 1149 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3081 CCAGACTCTGG
XhoI 668 CTCGAG C^TCGAG
1346 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaBI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SexAI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII