Restriction map for
pF1KA0756
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# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AclI AgeI Alw44I
AlwNI AocI ApaLI AscI AseI
AsnI Asp718I AviII BbeI BcgI
BclI BglII BlpI Bpu1102I BsaBI
BsiWI BspDI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI Eco81I EcoRI EcoRV
FbaI FseI FspI KasI KpnI
KspI MamI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI ScaI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 2446 TCCGGA T^CCGGA
AcyI 2232 GACGCC G[GA]^CG[CT]C
AflII 711 CTTAAG C^TTAAG
AhdI 2980 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2141 AGCGCT AGC^GCT
ApaI 2415 GGGCCC GGGCC^C
Asp700I 660 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2980 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2159 GAGCAC G[AT]GC[AT]^C
AvrII 2791 CCTAGG C^CTAGG
BbvCI 3006 CCTCAGC CCTCAGC(-5/-2)
BfrI 711 CTTAAG C^TTAAG
BlnI 2791 CCTAGG C^CTAGG
BsaHI 2232 GACGCC G[GA]^CG[CT]C
BseAI 2446 TCCGGA T^CCGGA
BsgI 1648 GTGCAG GTGCAG(16/14)
BsiEI 304 CGGCCG CG[GA][CT]^CG
BsiHKAI 2159 GAGCAC G[AT]GC[AT]^C
BsmBI 1695 CGTCTC CGTCTC(1/5)
Bsp1407I 731 TGTACA T^GTACA
BspEI 2446 TCCGGA T^CCGGA
BspLU11I 728 ACATGT A^CATGT
BsrDI 2094 CATTGC GCAATG(2/0)
BsrFI 1874 ACCGGC [GA]^CCGG[CT]
BsrGI 731 TGTACA T^GTACA
BstAPI 594 GCAGGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2650 GGTGACC G^GT[ACGT]ACC
BstPI 2650 GGTGACC G^GT[ACGT]ACC
Cfr10I 1874 ACCGGC [GA]^CCGG[CT]
EagI 304 CGGCCG C^GGCCG
Eam1105I 2980 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 304 CGGCCG C^GGCCG
Eco47III 2141 AGCGCT AGC^GCT
Eco52I 304 CGGCCG C^GGCCG
EcoNI 1836 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2650 GGTGACC G^GT[ACGT]ACC
EcoT22I 834 ATGCAT ATGCA^T
Hin1I 2232 GACGCC G[GA]^CG[CT]C
HpaI 2041 GTTAAC GTT^AAC
MroI 2446 TCCGGA T^CCGGA
MspA1I 1651 CAGCTG C[AC]G^C[GT]G
NcoI 518 CCATGG C^CATGG
NsiI 834 ATGCAT ATGCA^T
PaeR7I 2303 CTCGAG C^TCGAG
PshAI 2358 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1651 CAGCTG CAG^CTG
SexAI 1622 ACCAGGT A^CC[AT]GGT
SfiI 1868 GGCCAGACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 731 TGTACA T^GTACA
XhoI 2303 CTCGAG C^TCGAG
XmnI 660 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 2365 GTCTAC GT^[AC][GT]AC
2533 GTCTAC
AcsI 77 AAATTC [GA]^AATT[CT]
349 AAATTT
ApoI 77 AAATTC [GA]^AATT[CT]
349 AAATTT
BamHI 374 GGATCC G^GATCC
1931 GGATCC
BbrPI 1803 CACGTG CAC^GTG
2226 CACGTG
BciVI 2878 GGATAC GTATCC(6/5)
774 GTATCC
BsmI 832 GAATGC GAATGC(1/-1)
2241 GAATGC
BspHI 2495 TCATGA T^CATGA
2870 TCATGA
BsrBI 1720 CCGCTC CCGCTC(-3/-3)
1783 GAGCGG
BstXI 989 CCAACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1544 CCAACATCCTGG
DraIII 133 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
2325 CACAGTGTG
HincII 2041 GTTAAC GT[CT]^[GA]AC
2074 GTCAAC
HindII 2041 GTTAAC GT[CT]^[GA]AC
2074 GTCAAC
HindIII 765 AAGCTT A^AGCTT
3074 AAGCTT
NspI 599 ACATGC [GA]CATG^[CT]
728 ACATGT
PflMI 1137 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1830 CCAAGACCTGG
PmaCI 1803 CACGTG CAC^GTG
2226 CACGTG
PmlI 1803 CACGTG CAC^GTG
2226 CACGTG
RcaI 2495 TCATGA T^CATGA
2870 TCATGA
SbfI 381 CCTGCAGG CCTGCA^GG
3297 CCTGCAGG
Sse8387I 381 CCTGCAGG CCTGCA^GG
3297 CCTGCAGG
StuI 1844 AGGCCT AGG^CCT
2309 AGGCCT
TfiI 221 GATTC G^A[AT]TC
1359 GATTC
Van91I 1137 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1830 CCAAGACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AspI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflFI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XcmI XhoII XmaCI XmaI