Restriction map for
pF1KA0815
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AclI AcsI AflII AhdI
AlwI AocI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbvCI BciVI BclI
BfrI BlnI BsiWI BsmBI Bsp1407I
BspHI BsrGI Bst1107I BstBI BstZ17I
Bsu36I CpoI DraI DrdI Eam1105I
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MfeI MluI MunI NdeI NotI
NruI NsiI NspV PacI PflMI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SapI SbfI ScaI SexAI
SfuI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tsp509I Van91I
XbaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 802 GTCGAC GT^[AC][GT]AC
AccIII 1353 TCCGGA T^CCGGA
AfaI 861 GTAC GT^AC
BbeI 637 GGCGCC GGCGC^C
BbsI 1984 GAAGAC GAAGAC(2/6)
BfaI 511 CTAG C^TAG
BglII 2036 AGATCT A^GATCT
BlpI 3623 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3623 GCTCAGC GC^T[ACGT]AGC
BpuAI 1984 GAAGAC GAAGAC(2/6)
BsaBI 669 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2077 GAGACC GGTCTC(1/5)
BseAI 1353 TCCGGA T^CCGGA
BsmI 175 GAATGC GAATGC(1/-1)
BspDI 796 ATCGAT AT^CGAT
BspEI 1353 TCCGGA T^CCGGA
BspLU11I 751 ACATGT A^CATGT
BssHII 64 GCGCGC G^CGCGC
BssSI 1018 CACGAG CACGAG(-5/-1)
BstEII 151 GGTGACC G^GT[ACGT]ACC
BstPI 151 GGTGACC G^GT[ACGT]ACC
BstYI 2036 AGATCT [GA]^GATC[CT]
CelII 3623 GCTCAGC GC^T[ACGT]AGC
ClaI 796 ATCGAT AT^CGAT
DraIII 3541 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 120 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 151 GGTGACC G^GT[ACGT]ACC
HincII 802 GTCGAC GT[CT]^[GA]AC
HindII 802 GTCGAC GT[CT]^[GA]AC
KasI 637 GGCGCC G^GCGCC
MaeI 511 CTAG C^TAG
MamI 669 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2036 AGATCT [GA]^GATC[CT]
MroI 1353 TCCGGA T^CCGGA
MseI 293 TTAA T^TAA
NarI 637 GGCGCC GG^CGCC
NheI 510 GCTAGC G^CTAGC
PaeR7I 1905 CTCGAG C^TCGAG
PshAI 2764 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 861 GTAC GT^AC
SacI 1908 GAGCTC GAGCT^C
SalI 802 GTCGAC G^TCGAC
SfoI 637 GGCGCC GGC^GCC
SmlI 1905 CTCGAG C^T[CT][GA]AG
Tru9I 293 TTAA T^TAA
XhoI 1905 CTCGAG C^TCGAG
XhoII 2036 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AatII 1150 GACGTC GACGT^C
2665 GACGTC
AgeI 206 ACCGGT A^CCGGT
3179 ACCGGT
Alw44I 747 GTGCAC G^TGCAC
840 GTGCAC
ApaLI 747 GTGCAC G^TGCAC
840 GTGCAC
BcgI 804 CGACGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
794 GCATCGATGTCG
BseRI 1048 GAGGAG GAGGAG(10/8)
1639 GAGGAG
BsrBI 1815 GAGCGG CCGCTC(-3/-3)
3666 GAGCGG
DpnII 781 GATC ^GATC
2037 GATC
EarI 1135 CTCTTC CTCTTC(1/4)
1168 GAAGAG
Ksp632I 1135 CTCTTC CTCTTC(1/4)
1168 GAAGAG
MboI 781 GATC ^GATC
2037 GATC
NcoI 576 CCATGG C^CATGG
714 CCATGG
NdeII 781 GATC ^GATC
2037 GATC
PinAI 206 ACCGGT A^CCGGT
3179 ACCGGT
Sau3AI 781 GATC ^GATC
2037 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AflIII AluI
AlwNI Aor51HI ApaI AspHI AspI
AvaI AvaII BalI BanI BanII
BbrPI BbvI BcnI BglI BmyI
BpmI BsaAI BsaHI BsaJI BsaWI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EagI EclXI Eco47III
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KspI MaeII
MaeIII MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NgoMIV NlaIII
NlaIV NspI PflFI PleI PmaCI
PmlI PpuMI PstI PvuII SacII
Sau96I ScrFI SfaNI SfcI SfiI
SgrAI SmaI SphI StuI StyI
TaqI TseI Tsp45I TspRI Tth111I
TthHB8I XcmI XmaCI XmaI