Restriction map for
pF1KA0819
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# NO CUT ENZYMES ( 66 enzymes) #
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AccIII AclI AflIII Aor51HI AscI
AseI AsnI AviII BbrPI BsaBI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FspI HpaI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SfiI SfuI SgrAI
SnaBI SspBI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AatII 2788 GACGTC GACGT^C
Acc65I 2724 GGTACC G^GTACC
AgeI 50 ACCGGT A^CCGGT
Asp718I 2724 GGTACC G^GTACC
AvrII 516 CCTAGG C^CTAGG
BamHI 2903 GGATCC G^GATCC
BclI 653 TGATCA T^GATCA
BglII 1623 AGATCT A^GATCT
BlnI 516 CCTAGG C^CTAGG
BsaAI 2314 TACGTG [CT]AC^GT[GA]
BsmBI 1055 CGTCTC CGTCTC(1/5)
BspHI 1784 TCATGA T^CATGA
DrdI 49 GACCGGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 653 TGATCA T^GATCA
FseI 4900 GGCCGGCC GGCCGG^CC
HindIII 166 AAGCTT A^AGCTT
KpnI 2724 GGTACC GGTAC^C
KspI 4505 CCGCGG CCGC^GG
PinAI 50 ACCGGT A^CCGGT
PshAI 3450 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1784 TCATGA T^CATGA
SacII 4505 CCGCGG CCGC^GG
SalI 5531 GTCGAC G^TCGAC
ScaI 2375 AGTACT AGT^ACT
SexAI 3713 ACCTGGT A^CC[AT]GGT
SpeI 1173 ACTAGT A^CTAGT
SphI 4535 GCATGC GCATG^C
StuI 2945 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccI 2311 GTCTAC GT^[AC][GT]AC
5531 GTCGAC
AccII 4400 CGCG CG^CG
4506 CGCG
AflII 5281 CTTAAG C^TTAAG
5578 CTTAAG
AhdI 3070 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5014 GACGACAAGTC
AspEI 3070 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5014 GACGACAAGTC
AspI 1396 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5152 GACGATGTC
BcgI 2177 CGACCCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4185 CGAGCCCAATGC
BciVI 473 GTATCC GTATCC(6/5)
1410 GTATCC
BfrI 5281 CTTAAG C^TTAAG
5578 CTTAAG
BsgI 4036 CTGCAC GTGCAG(16/14)
5449 GTGCAG
BsmI 2205 GAATGC GAATGC(1/-1)
5220 GAATGC
BspMI 70 ACCTGC ACCTGC(4/8)
5325 GCAGGT
BsrBI 138 CCGCTC CCGCTC(-3/-3)
2665 GAGCGG
BsrDI 1752 CATTGC GCAATG(2/0)
1541 GCAATG
BstUI 4400 CGCG CG^CG
4506 CGCG
DraIII 258 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
5071 CACAGGGTG
Eam1105I 3070 GACAGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5014 GACGACAAGTC
HgaI 4467 GACGC GACGC(5/10)
4651 GCGTC
HincII 1426 GTCAAC GT[CT]^[GA]AC
5531 GTCGAC
HindII 1426 GTCAAC GT[CT]^[GA]AC
5531 GTCGAC
MvnI 4400 CGCG CG^CG
4506 CGCG
NaeI 4682 GCCGGC GCC^GGC
4901 GCCGGC
NgoMIV 4682 GCCGGC G^CCGGC
4901 GCCGGC
NspI 2287 ACATGC [GA]CATG^[CT]
4535 GCATGC
PflFI 1396 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5152 GACGATGTC
PflMI 1631 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2181 CCAGCTGCTGG
SbfI 2532 CCTGCAGG CCTGCA^GG
4362 CCTGCAGG
Sse8387I 2532 CCTGCAGG CCTGCA^GG
4362 CCTGCAGG
Tth111I 1396 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5152 GACGATGTC
Van91I 1631 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2181 CCAGCTGCTGG
XcmI 3659 CCAAGGACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4190 CCAATGCCTCGGTGG
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# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI XmnI