Restriction map for
pF1KA0835
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# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BbeI
BcgI BclI BfrI BlnI BsaBI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoRI FbaI FseI HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I RcaI RsrII SacII SalI
SapI SbfI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 354 GACGTC GACGT^C
AccI 1987 GTAGAC GT^[AC][GT]AC
AccII 1913 CGCG CG^CG
Alw44I 583 GTGCAC G^TGCAC
Aor51HI 689 AGCGCT AGC^GCT
ApaI 987 GGGCCC GGGCC^C
ApaLI 583 GTGCAC G^TGCAC
AviII 1175 TGCGCA TGC^GCA
BbrPI 1486 CACGTG CAC^GTG
BciVI 2322 GTATCC GTATCC(6/5)
BlpI 199 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 199 GCTGAGC GC^T[ACGT]AGC
BsaAI 1486 CACGTG [CT]AC^GT[GA]
BsiEI 1617 CGATCG CG[GA][CT]^CG
BsmBI 352 GAGACG CGTCTC(1/5)
BspLU11I 3287 ACATGT A^CATGT
BsrDI 1533 CATTGC GCAATG(2/0)
BssSI 2606 CTCGTG CACGAG(-5/-1)
BstEII 1746 GGTCACC G^GT[ACGT]ACC
BstPI 1746 GGTCACC G^GT[ACGT]ACC
BstUI 1913 CGCG CG^CG
CelII 199 GCTGAGC GC^T[ACGT]AGC
DraIII 205 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 689 AGCGCT AGC^GCT
EcoO65I 1746 GGTCACC G^GT[ACGT]ACC
EcoRV 1978 GATATC GAT^ATC
EcoT22I 1435 ATGCAT ATGCA^T
FspI 1175 TGCGCA TGC^GCA
HincII 1080 GTCAAC GT[CT]^[GA]AC
HindII 1080 GTCAAC GT[CT]^[GA]AC
MseI 2351 TTAA T^TAA
MvnI 1913 CGCG CG^CG
NdeI 1667 CATATG CA^TATG
NsiI 1435 ATGCAT ATGCA^T
PaeR7I 1654 CTCGAG C^TCGAG
PmaCI 1486 CACGTG CAC^GTG
PmlI 1486 CACGTG CAC^GTG
PshAI 700 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1617 CGATCG CGAT^CG
SexAI 206 ACCTGGT A^CC[AT]GGT
SfiI 1710 GGCCAACTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 2162 AGGCCT AGG^CCT
Tru9I 2351 TTAA T^TAA
XhoI 1654 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 2304 GAATTT [GA]^AATT[CT]
3255 GAATTT
AflIII 2987 ACGTGT A^C[GA][CT]GT
3287 ACATGT
AocI 1626 CCTCAGG CC^T[ACGT]AGG
2233 CCTGAGG
ApoI 2304 GAATTT [GA]^AATT[CT]
3255 GAATTT
BanI 140 GGCACC G^G[CT][GA]CC
2634 GGCACC
BbsI 16 GAAGAC GAAGAC(2/6)
943 GAAGAC
BfaI 2210 CTAG C^TAG
2981 CTAG
BglII 1424 AGATCT A^GATCT
3092 AGATCT
BpuAI 16 GAAGAC GAAGAC(2/6)
943 GAAGAC
BsaWI 2585 ACCGGA [AT]^CCGG[AT]
2891 ACCGGA
BspMI 587 ACCTGC ACCTGC(4/8)
2833 ACCTGC
BsrBI 1038 CCGCTC CCGCTC(-3/-3)
2314 GAGCGG
BsrFI 1679 ACCGGC [GA]^CCGG[CT]
2186 ACCGGC
BstXI 1721 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2865 CCACGCCAATGG
Bsu36I 1626 CCTCAGG CC^T[ACGT]AGG
2233 CCTGAGG
Cfr10I 1679 ACCGGC [GA]^CCGG[CT]
2186 ACCGGC
DsaI 3071 CCATGG C^C[GA][CT]GG
3101 CCATGG
Eco81I 1626 CCTCAGG CC^T[ACGT]AGG
2233 CCTGAGG
MaeI 2210 CTAG C^TAG
2981 CTAG
NcoI 3071 CCATGG C^CATGG
3101 CCATGG
PflMI 568 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
711 CCAGTCCCTGG
SacI 26 GAGCTC GAGCT^C
979 GAGCTC
ScaI 131 AGTACT AGT^ACT
1016 AGTACT
SmaI 1121 CCCGGG CCC^GGG
2844 CCCGGG
SphI 1793 GCATGC GCATG^C
2015 GCATGC
Van91I 568 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
711 CCAGTCCCTGG
XmaCI 1121 CCCGGG C^CCGGG
2844 CCCGGG
XmaI 1121 CCCGGG C^CCGGG
2844 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BamHI BanII BbvCI BbvI BcnI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII