Restriction map for
pF1KA0890
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# NO CUT ENZYMES ( 83 enzymes) #
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AatII Acc65I AclI AcsI AflII
AflIII AgeI AocI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BbrPI BbvCI BclI BfrI
BglII BlnI BsaBI BsiEI BsiWI
Bsp1407I BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I CpoI
DraI EagI EclXI Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI HindIII
HpaI KpnI KspI MamI MfeI
MluI MunI NcoI NdeI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SbfI ScaI SfuI SgrAI
SnaBI Sse8387I SspBI SspI SwaI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccIII 465 TCCGGA T^CCGGA
AhdI 616 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 616 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2009 GGATCC G^GATCC
BciVI 1613 GTATCC GTATCC(6/5)
BlpI 325 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 325 GCTGAGC GC^T[ACGT]AGC
BsaAI 226 TACGTG [CT]AC^GT[GA]
BsaWI 465 TCCGGA [AT]^CCGG[AT]
BseAI 465 TCCGGA T^CCGGA
BsmBI 1234 CGTCTC CGTCTC(1/5)
BsmI 1939 GAATGC GAATGC(1/-1)
BspEI 465 TCCGGA T^CCGGA
BspHI 1766 TCATGA T^CATGA
BssSI 2083 CACGAG CACGAG(-5/-1)
BstEII 3102 GGTCACC G^GT[ACGT]ACC
BstPI 3102 GGTCACC G^GT[ACGT]ACC
CelII 325 GCTGAGC GC^T[ACGT]AGC
DraIII 1348 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2925 CCACGG C^C[GA][CT]GG
Eam1105I 616 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3102 GGTCACC G^GT[ACGT]ACC
FseI 3060 GGCCGGCC GGCCGG^CC
MroI 465 TCCGGA T^CCGGA
PaeR7I 119 CTCGAG C^TCGAG
RcaI 1766 TCATGA T^CATGA
SalI 3177 GTCGAC G^TCGAC
SfiI 2341 GGCCGGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 219 ACTAGT A^CTAGT
SphI 2078 GCATGC GCATG^C
StuI 2963 AGGCCT AGG^CCT
Tsp509I 2878 AATT ^AATT
XhoI 119 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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Aor51HI 1418 AGCGCT AGC^GCT
1790 AGCGCT
BbeI 881 GGCGCC GGCGC^C
3338 GGCGCC
BsaI 1018 GAGACC GGTCTC(1/5)
1075 GAGACC
BspDI 319 ATCGAT AT^CGAT
1984 ATCGAT
BsrDI 2196 CATTGC GCAATG(2/0)
1451 GCAATG
BstYI 2009 GGATCC [GA]^GATC[CT]
2429 AGATCC
ClaI 319 ATCGAT AT^CGAT
1984 ATCGAT
Eco47III 1418 AGCGCT AGC^GCT
1790 AGCGCT
HincII 91 GTCAAC GT[CT]^[GA]AC
3177 GTCGAC
HindII 91 GTCAAC GT[CT]^[GA]AC
3177 GTCGAC
KasI 881 GGCGCC G^GCGCC
3338 GGCGCC
MflI 2009 GGATCC [GA]^GATC[CT]
2429 AGATCC
MseI 139 TTAA T^TAA
3185 TTAA
NarI 881 GGCGCC GG^CGCC
3338 GGCGCC
NheI 423 GCTAGC G^CTAGC
3288 GCTAGC
PflMI 804 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1512 CCACGAACTGG
SexAI 2001 ACCAGGT A^CC[AT]GGT
2453 ACCTGGT
SfoI 881 GGCGCC GGC^GCC
3338 GGCGCC
SmaI 2720 CCCGGG CCC^GGG
2870 CCCGGG
Tru9I 139 TTAA T^TAA
3185 TTAA
Van91I 804 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1512 CCACGAACTGG
XcmI 734 CCAAGAACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
983 CCATGTATAACCTGG
XhoII 2009 GGATCC [GA]^GATC[CT]
2429 AGATCC
XmaCI 2720 CCCGGG C^CCGGG
2870 CCCGGG
XmaI 2720 CCCGGG C^CCGGG
2870 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII AviII
BalI BanI BanII BbsI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I