Restriction map for
pF1KA0909
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflIII AgeI AocI ApoI
AscI AseI AsnI Asp718I AviII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BlpI Bpu1102I BsaAI BsaBI
BsiWI Bsp1407I BspDI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstAPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI EclXI Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HpaI KasI KpnI
KspI MamI MfeI MluI MunI
MvnI NarI NheI NotI NruI
NsiI NspI PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I XbaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 1910 TCCGGA T^CCGGA
AcyI 1014 GACGCC G[GA]^CG[CT]C
AflII 3277 CTTAAG C^TTAAG
AhdI 1882 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 430 GTGCAC G^TGCAC
Aor51HI 3236 AGCGCT AGC^GCT
ApaLI 430 GTGCAC G^TGCAC
Asp700I 3577 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1882 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1048 CCTAGG C^CTAGG
BfrI 3277 CTTAAG C^TTAAG
BglI 1017 GCCCACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 50 AGATCT A^GATCT
BlnI 1048 CCTAGG C^CTAGG
BsaHI 1014 GACGCC G[GA]^CG[CT]C
BseAI 1910 TCCGGA T^CCGGA
BsiEI 504 CGACCG CG[GA][CT]^CG
BsmBI 2514 CGTCTC CGTCTC(1/5)
BsmI 2351 GCATTC GAATGC(1/-1)
BspEI 1910 TCCGGA T^CCGGA
BspHI 3515 TCATGA T^CATGA
BsrFI 3191 GCCGGC [GA]^CCGG[CT]
BssSI 3127 CACGAG CACGAG(-5/-1)
BstBI 2038 TTCGAA TT^CGAA
BstEII 262 GGTTACC G^GT[ACGT]ACC
BstPI 262 GGTTACC G^GT[ACGT]ACC
BstYI 50 AGATCT [GA]^GATC[CT]
Cfr10I 3191 GCCGGC [GA]^CCGG[CT]
DraIII 619 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2112 CCATGG C^C[GA][CT]GG
Eam1105I 1882 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3236 AGCGCT AGC^GCT
EcoO65I 262 GGTTACC G^GT[ACGT]ACC
Hin1I 1014 GACGCC G[GA]^CG[CT]C
HincII 2239 GTTGAC GT[CT]^[GA]AC
HindII 2239 GTTGAC GT[CT]^[GA]AC
HindIII 2217 AAGCTT A^AGCTT
MflI 50 AGATCT [GA]^GATC[CT]
MroI 1910 TCCGGA T^CCGGA
MslI 1710 CATCGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 3191 GCCGGC GCC^GGC
NcoI 2112 CCATGG C^CATGG
NdeI 1166 CATATG CA^TATG
NgoMIV 3191 GCCGGC G^CCGGC
NspV 2038 TTCGAA TT^CGAA
PaeR7I 941 CTCGAG C^TCGAG
PshAI 2497 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3515 TCATGA T^CATGA
ScaI 1870 AGTACT AGT^ACT
SexAI 620 ACCTGGT A^CC[AT]GGT
SfuI 2038 TTCGAA TT^CGAA
XhoI 941 CTCGAG C^TCGAG
XhoII 50 AGATCT [GA]^GATC[CT]
XmnI 3577 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 1810 GGGCCC GGGCC^C
2713 GGGCCC
AspI 418 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2940 GACCATGTC
BalI 2954 TGGCCA TGG^CCA
3599 TGGCCA
BanI 1980 GGTGCC G^G[CT][GA]CC
2542 GGCACC
BbsI 291 GAAGAC GAAGAC(2/6)
80 GTCTTC
BclI 152 TGATCA T^GATCA
1704 TGATCA
BpuAI 291 GAAGAC GAAGAC(2/6)
80 GTCTTC
BsaI 2182 GAGACC GGTCTC(1/5)
2938 GAGACC
FbaI 152 TGATCA T^GATCA
1704 TGATCA
MluNI 2954 TGGCCA TGG^CCA
3599 TGGCCA
MscI 2954 TGGCCA TGG^CCA
3599 TGGCCA
PflFI 418 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2940 GACCATGTC
PleI 2359 GACTC GAGTC(4/5)
3158 GAGTC
PstI 474 CTGCAG CTGCA^G
684 CTGCAG
SacI 844 GAGCTC GAGCT^C
1354 GAGCTC
SmaI 530 CCCGGG CCC^GGG
2396 CCCGGG
TfiI 1853 GATTC G^A[AT]TC
3521 GATTC
Tth111I 418 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2940 GACCATGTC
XmaCI 530 CCCGGG C^CCGGG
2396 CCCGGG
XmaI 530 CCCGGG C^CCGGG
2396 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI