Restriction map for
pF1KA0947
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# NO CUT ENZYMES ( 63 enzymes) #
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AclI AflII Alw44I Aor51HI ApaLI
AscI AviII AvrII BamHI BbrPI
BbvCI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsiEI BsiWI Bsp1407I
BsrBI BsrGI BssHII BstBI BstEII
BstPI CelII CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I FseI
FspI KspI MluI NaeI NgoMIV
NheI NotI NspV PacI PflMI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Van91I
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 3456 GACGTC GACGT^C
Acc65I 3274 GGTACC G^GTACC
AccII 53 CGCG CG^CG
AccIII 6066 TCCGGA T^CCGGA
AgeI 2686 ACCGGT A^CCGGT
AhdI 5551 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 5285 GGGCCC GGGCC^C
Asp718I 3274 GGTACC G^GTACC
AspEI 5551 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 27 GGCGCC GGCGC^C
BcgI 1516 CGAGGTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 6066 TCCGGA T^CCGGA
BspDI 4661 ATCGAT AT^CGAT
BspEI 6066 TCCGGA T^CCGGA
BspHI 963 TCATGA T^CATGA
BsrFI 2686 ACCGGT [GA]^CCGG[CT]
BstUI 53 CGCG CG^CG
Cfr10I 2686 ACCGGT [GA]^CCGG[CT]
ClaI 4661 ATCGAT AT^CGAT
Eam1105I 5551 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 664 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1524 ATGCAT ATGCA^T
FauI 5495 GCGGG CCCGC(4/6)
HaeII 27 GGCGCC [GA]GCGC^[CT]
HindIII 6707 AAGCTT A^AGCTT
HpaI 150 GTTAAC GTT^AAC
KasI 27 GGCGCC G^GCGCC
KpnI 3274 GGTACC GGTAC^C
MroI 6066 TCCGGA T^CCGGA
MvnI 53 CGCG CG^CG
NarI 27 GGCGCC GG^CGCC
NdeI 584 CATATG CA^TATG
NruI 52 TCGCGA TCG^CGA
NsiI 1524 ATGCAT ATGCA^T
PinAI 2686 ACCGGT A^CCGGT
PshAI 6664 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 963 TCATGA T^CATGA
SapI 2154 GAAGAGC GCTCTTC(1/4)
SfoI 27 GGCGCC GGC^GCC
SphI 6603 GCATGC GCATG^C
StuI 6722 AGGCCT AGG^CCT
XbaI 1765 TCTAGA T^CTAGA
XcmI 1935 CCAGTCTTCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 27 GGCGCC G[GA]^CG[CT]C
3456 GACGTC
AflIII 4302 ACATGT A^C[GA][CT]GT
6101 ACATGT
AocI 2959 CCTCAGG CC^T[ACGT]AGG
5230 CCTCAGG
BsaHI 27 GGCGCC G[GA]^CG[CT]C
3456 GACGTC
BsaI 13 GAGACC GGTCTC(1/5)
3429 GAGACC
BsmBI 5132 CGTCTC CGTCTC(1/5)
3311 GAGACG
BspLU11I 4302 ACATGT A^CATGT
6101 ACATGT
BssSI 5327 CACGAG CACGAG(-5/-1)
350 CTCGTG
Bst1107I 6477 GTATAC GTA^TAC
6632 GTATAC
BstAPI 5404 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6466 GCAAACATTGC
BstXI 2966 CCACAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6695 CCAAGATCCTGG
BstZ17I 6477 GTATAC GTA^TAC
6632 GTATAC
Bsu36I 2959 CCTCAGG CC^T[ACGT]AGG
5230 CCTCAGG
Eco81I 2959 CCTCAGG CC^T[ACGT]AGG
5230 CCTCAGG
EcoRI 2536 GAATTC G^AATTC
5238 GAATTC
EcoRV 4959 GATATC GAT^ATC
5946 GATATC
Hin1I 27 GGCGCC G[GA]^CG[CT]C
3456 GACGTC
MfeI 998 CAATTG C^AATTG
6532 CAATTG
MunI 998 CAATTG C^AATTG
6532 CAATTG
PaeR7I 1324 CTCGAG C^TCGAG
1514 CTCGAG
SexAI 663 ACCTGGT A^CC[AT]GGT
4310 ACCAGGT
SmaI 31 CCCGGG CCC^GGG
1071 CCCGGG
SspI 5314 AATATT AAT^ATT
6571 AATATT
XhoI 1324 CTCGAG C^TCGAG
1514 CTCGAG
XmaCI 31 CCCGGG C^CCGGG
1071 CCCGGG
XmaI 31 CCCGGG C^CCGGG
1071 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvI
BciVI BclI BcnI BfaI BglII
BmyI BpmI BpuAI BsaBI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI PshBI PstI PvuII
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII
XmnI