Restriction map for
pF1KA0954
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# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
AscI AseI AsnI Asp718I AspEI
AvrII BamHI BciVI BfrI BlnI
BsiWI BsmI BspDI BspHI BssSI
Bst1107I BstAPI BstZ17I ClaI CpoI
DraI DrdI Eam1105I EcoRV EcoT22I
FseI HincII HindII HindIII HpaI
KpnI KspI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PmeI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SfiI SnaBI SpeI SspI
SwaI XbaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 2392 GTAGAC GT^[AC][GT]AC
AccIII 2262 TCCGGA T^CCGGA
AgeI 3311 ACCGGT A^CCGGT
Asp700I 1197 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 1660 GTCTTC GAAGAC(2/6)
BbvCI 3171 CCTCAGC CCTCAGC(-5/-2)
BcgI 861 GCACCCCACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 821 TGATCA T^GATCA
BfaI 2693 CTAG C^TAG
BpuAI 1660 GTCTTC GAAGAC(2/6)
BsaBI 3075 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1447 GAGACC GGTCTC(1/5)
BseAI 2262 TCCGGA T^CCGGA
BspEI 2262 TCCGGA T^CCGGA
BspLU11I 2624 ACATGT A^CATGT
BssHII 1767 GCGCGC G^CGCGC
BstBI 40 TTCGAA TT^CGAA
BstEII 2571 GGTGACC G^GT[ACGT]ACC
BstPI 2571 GGTGACC G^GT[ACGT]ACC
DraIII 1300 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 993 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2571 GGTGACC G^GT[ACGT]ACC
FbaI 821 TGATCA T^GATCA
MaeI 2693 CTAG C^TAG
MamI 3075 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1807 CAATTG C^AATTG
MroI 2262 TCCGGA T^CCGGA
MunI 1807 CAATTG C^AATTG
NspV 40 TTCGAA TT^CGAA
PaeR7I 869 CTCGAG C^TCGAG
PinAI 3311 ACCGGT A^CCGGT
PpuMI 2384 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1899 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 739 GAGCTC GAGCT^C
ScaI 3092 AGTACT AGT^ACT
SexAI 2387 ACCTGGT A^CC[AT]GGT
SfuI 40 TTCGAA TT^CGAA
SgrAI 3310 CACCGGTG C[GA]^CCGG[CT]G
TfiI 778 GAATC G^A[AT]TC
XhoI 869 CTCGAG C^TCGAG
XmnI 1197 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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AcsI 3069 GAATTC [GA]^AATT[CT]
3343 GAATTC
AflIII 1779 ACACGT A^C[GA][CT]GT
2624 ACATGT
Alw44I 1717 GTGCAC G^TGCAC
1837 GTGCAC
AocI 1936 CCTGAGG CC^T[ACGT]AGG
3228 CCTGAGG
ApaLI 1717 GTGCAC G^TGCAC
1837 GTGCAC
ApoI 3069 GAATTC [GA]^AATT[CT]
3343 GAATTC
AspI 1654 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1960 GACATGGTC
AviII 473 TGCGCA TGC^GCA
2372 TGCGCA
BbeI 254 GGCGCC GGCGC^C
2164 GGCGCC
BbrPI 502 CACGTG CAC^GTG
1780 CACGTG
BglII 1565 AGATCT A^GATCT
1928 AGATCT
BsiEI 1671 CGGCCG CG[GA][CT]^CG
1696 CGGCCG
BsmBI 16 GAGACG CGTCTC(1/5)
2077 GAGACG
Bsp1407I 614 TGTACA T^GTACA
1217 TGTACA
BsrBI 904 CCGCTC CCGCTC(-3/-3)
2757 CCGCTC
BsrDI 939 CATTGC GCAATG(2/0)
2307 CATTGC
BsrGI 614 TGTACA T^GTACA
1217 TGTACA
Bsu36I 1936 CCTGAGG CC^T[ACGT]AGG
3228 CCTGAGG
EagI 1671 CGGCCG C^GGCCG
1696 CGGCCG
EclXI 1671 CGGCCG C^GGCCG
1696 CGGCCG
Eco52I 1671 CGGCCG C^GGCCG
1696 CGGCCG
Eco81I 1936 CCTGAGG CC^T[ACGT]AGG
3228 CCTGAGG
EcoRI 3069 GAATTC G^AATTC
3343 GAATTC
FspI 473 TGCGCA TGC^GCA
2372 TGCGCA
KasI 254 GGCGCC G^GCGCC
2164 GGCGCC
MseI 1334 TTAA T^TAA
2711 TTAA
NarI 254 GGCGCC GG^CGCC
2164 GGCGCC
PflFI 1654 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1960 GACATGGTC
PmaCI 502 CACGTG CAC^GTG
1780 CACGTG
PmlI 502 CACGTG CAC^GTG
1780 CACGTG
SfoI 254 GGCGCC GGC^GCC
2164 GGCGCC
SphI 1226 GCATGC GCATG^C
1826 GCATGC
SspBI 614 TGTACA T^GTACA
1217 TGTACA
Tru9I 1334 TTAA T^TAA
2711 TTAA
Tth111I 1654 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1960 GACATGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI RsaI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmaI
SmlI Sse8387I StuI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI