Restriction map for
pF1KA0987
##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AviII BamHI
BbrPI BfrI BglI BsiWI Bsp1407I
BspDI BspHI BsrDI BsrGI Bst1107I
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DsaI Eco47III
Eco81I EcoO65I FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MfeI MluI MunI NaeI NcoI
NgoMIV NheI NotI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshBI PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SfiI
SgrAI SmaI SpeI Sse8387I SspBI
SspI SwaI XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AclI 1307 AACGTT AA^CGTT
AflIII 3197 ACATGT A^C[GA][CT]GT
AhdI 634 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 634 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2487 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2043 CCCGAG C^[CT]CG[GA]G
AvrII 1695 CCTAGG C^CTAGG
BalI 1350 TGGCCA TGG^CCA
BanI 90 GGCGCC G^G[CT][GA]CC
BbeI 90 GGCGCC GGCGC^C
BbvCI 1420 GCTGAGG CCTCAGC(-5/-2)
BcgI 2915 CGAAGGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1810 GGATAC GTATCC(6/5)
BclI 1391 TGATCA T^GATCA
BglII 2988 AGATCT A^GATCT
BlnI 1695 CCTAGG C^CTAGG
BsaAI 459 TACGTA [CT]AC^GT[GA]
BsaBI 895 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2506 GAGACG CGTCTC(1/5)
BsmI 2653 GCATTC GAATGC(1/-1)
BsoBI 2043 CCCGAG C^[CT]CG[GA]G
BspLU11I 3197 ACATGT A^CATGT
BsrFI 974 ACCGGC [GA]^CCGG[CT]
BssHII 81 GCGCGC G^CGCGC
BssSI 1495 CACGAG CACGAG(-5/-1)
BstBI 524 TTCGAA TT^CGAA
BstXI 3209 CCAAAGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 974 ACCGGC [GA]^CCGG[CT]
DraII 128 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 2349 GACTAAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1054 CGGCCG C^GGCCG
Eam1105I 634 GACATCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1054 CGGCCG C^GGCCG
Eco52I 1054 CGGCCG C^GGCCG
EcoO109I 128 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 2428 GAATTC G^AATTC
EcoRV 595 GATATC GAT^ATC
EcoT22I 850 ATGCAT ATGCA^T
FbaI 1391 TGATCA T^GATCA
HgaI 831 GACGC GACGC(5/10)
KasI 90 GGCGCC G^GCGCC
MamI 895 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1350 TGGCCA TGG^CCA
MscI 1350 TGGCCA TGG^CCA
NarI 90 GGCGCC GG^CGCC
NdeI 2957 CATATG CA^TATG
NruI 969 TCGCGA TCG^CGA
NsiI 850 ATGCAT ATGCA^T
NspV 524 TTCGAA TT^CGAA
PflFI 2487 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1525 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1307 AACGTT AA^CGTT
SapI 1250 GAAGAGC GCTCTTC(1/4)
SexAI 601 ACCAGGT A^CC[AT]GGT
SfoI 90 GGCGCC GGC^GCC
SfuI 524 TTCGAA TT^CGAA
SnaBI 459 TACGTA TAC^GTA
SphI 326 GCATGC GCATG^C
StuI 1687 AGGCCT AGG^CCT
Tth111I 2487 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 643 TCCGGA T^CCGGA
1407 TCCGGA
AcyI 90 GGCGCC G[GA]^CG[CT]C
831 GACGCC
BlpI 1639 GCTGAGC GC^T[ACGT]AGC
2535 GCTTAGC
Bpu1102I 1639 GCTGAGC GC^T[ACGT]AGC
2535 GCTTAGC
BsaHI 90 GGCGCC G[GA]^CG[CT]C
831 GACGCC
BsaWI 643 TCCGGA [AT]^CCGG[AT]
1407 TCCGGA
BseAI 643 TCCGGA T^CCGGA
1407 TCCGGA
BspEI 643 TCCGGA T^CCGGA
1407 TCCGGA
BspMI 1646 ACCTGC ACCTGC(4/8)
2528 ACCTGC
BstAPI 326 GCATGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2523 GCACCACCTGC
BstYI 1049 AGATCC [GA]^GATC[CT]
2988 AGATCT
CelII 1639 GCTGAGC GC^T[ACGT]AGC
2535 GCTTAGC
EcoNI 1690 CCTTACCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2739 CCTGGAACAGG
Hin1I 90 GGCGCC G[GA]^CG[CT]C
831 GACGCC
MaeII 460 ACGT A^CGT
1308 ACGT
MflI 1049 AGATCC [GA]^GATC[CT]
2988 AGATCT
MroI 643 TCCGGA T^CCGGA
1407 TCCGGA
NspI 326 GCATGC [GA]CATG^[CT]
3197 ACATGT
PflMI 3150 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3244 CCAGAAGATGG
PstI 152 CTGCAG CTGCA^G
2639 CTGCAG
Van91I 3150 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3244 CCAGAAGATGG
XhoII 1049 AGATCC [GA]^GATC[CT]
2988 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I