Restriction map for
pF1KA1008
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BglI BlnI BsaAI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoT22I
FbaI HaeII Hin1I HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI NarI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1342 GTAGAC GT^[AC][GT]AC
AccII 61 CGCG CG^CG
AlwNI 171 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1317 CCTGAGG CC^T[ACGT]AGG
Asp700I 1697 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 64 GAGCAC G[AT]GC[AT]^C
AviII 106 TGCGCA TGC^GCA
BglII 1857 AGATCT A^GATCT
BmyI 64 GAGCAC G[AGT]GC[ACT]^C
BsaI 969 GAGACC GGTCTC(1/5)
BsgI 220 CTGCAC GTGCAG(16/14)
BsiHKAI 64 GAGCAC G[AT]GC[AT]^C
Bsp1286I 64 GAGCAC G[AGT]GC[ACT]^C
BspHI 1944 TCATGA T^CATGA
BsrFI 135 GCCGGC [GA]^CCGG[CT]
BsrI 2418 CCAGT ACTGG(1/-1)
BssSI 127 CACGAG CACGAG(-5/-1)
BstBI 1973 TTCGAA TT^CGAA
BstUI 61 CGCG CG^CG
BstXI 2063 CCAAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1317 CCTGAGG CC^T[ACGT]AGG
Cfr10I 135 GCCGGC [GA]^CCGG[CT]
DraI 756 TTTAAA TTT^AAA
DraIII 1153 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1317 CCTGAGG CC^T[ACGT]AGG
EcoRI 1055 GAATTC G^AATTC
EcoRV 1139 GATATC GAT^ATC
FseI 134 GGCCGGCC GGCCGG^CC
FspI 106 TGCGCA TGC^GCA
HincII 2319 GTTGAC GT[CT]^[GA]AC
HindII 2319 GTTGAC GT[CT]^[GA]AC
MspA1I 412 CAGCTG C[AC]G^C[GT]G
MvnI 61 CGCG CG^CG
NaeI 135 GCCGGC GCC^GGC
NcoI 1892 CCATGG C^CATGG
NdeI 1676 CATATG CA^TATG
NgoMIV 135 GCCGGC G^CCGGC
NheI 667 GCTAGC G^CTAGC
NspV 1973 TTCGAA TT^CGAA
PstI 1861 CTGCAG CTGCA^G
PvuII 412 CAGCTG CAG^CTG
RcaI 1944 TCATGA T^CATGA
SfuI 1973 TTCGAA TT^CGAA
SpeI 1732 ACTAGT A^CTAGT
SphI 983 GCATGC GCATG^C
Tsp45I 1568 GTGAC ^GT[GC]AC
XcmI 1267 CCAAAGATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1697 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 37 enzymes) #
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AflII 609 CTTAAG C^TTAAG
898 CTTAAG
AseI 1654 ATTAAT AT^TAAT
2496 ATTAAT
AsnI 1654 ATTAAT AT^TAAT
2496 ATTAAT
AvaI 32 CCCGGG C^[CT]CG[GA]G
100 CCCGGG
BbsI 1016 GAAGAC GAAGAC(2/6)
1312 GAAGAC
BfrI 609 CTTAAG C^TTAAG
898 CTTAAG
BlpI 1539 GCTTAGC GC^T[ACGT]AGC
2207 GCTTAGC
Bpu1102I 1539 GCTTAGC GC^T[ACGT]AGC
2207 GCTTAGC
BpuAI 1016 GAAGAC GAAGAC(2/6)
1312 GAAGAC
BsaBI 48 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2191 GATTTTCATC
BsmBI 2213 CGTCTC CGTCTC(1/5)
77 GAGACG
BsoBI 32 CCCGGG C^[CT]CG[GA]G
100 CCCGGG
BspMI 71 ACCTGC ACCTGC(4/8)
1032 ACCTGC
BstF5I 1981 CATCC GGATG(2/0)
1354 GGATG
BstYI 1857 AGATCT [GA]^GATC[CT]
2675 AGATCC
CelII 1539 GCTTAGC GC^T[ACGT]AGC
2207 GCTTAGC
DraII 158 AGGACCC [GA]G^G[ACGT]CC[CT]
250 GGGACCT
DsaI 1740 CCGTGG C^C[GA][CT]GG
1892 CCATGG
EarI 880 GAAGAG CTCTTC(1/4)
1484 GAAGAG
EcoO109I 158 AGGACCC [GA]G^G[ACGT]CC[CT]
250 GGGACCT
FauI 180 CCCGC CCCGC(4/6)
37 GCGGG
FokI 1981 CATCC GGATG(9/13)
1354 GGATG
HgaI 173 GCGTC GACGC(5/10)
2212 GCGTC
HindIII 2335 AAGCTT A^AGCTT
2773 AAGCTT
HphI 1171 GGTGA GGTGA(8/7)
2239 TCACC
Ksp632I 880 GAAGAG CTCTTC(1/4)
1484 GAAGAG
MamI 48 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2191 GATTTTCATC
MflI 1857 AGATCT [GA]^GATC[CT]
2675 AGATCC
NspI 983 GCATGC [GA]CATG^[CT]
1962 ACATGC
PflMI 973 CCATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2503 CCAAAGTATGG
PpuMI 158 AGGACCC [GA]G^G[AT]CC[CT]
250 GGGACCT
PshBI 1654 ATTAAT AT^TAAT
2496 ATTAAT
SmaI 32 CCCGGG CCC^GGG
100 CCCGGG
Van91I 973 CCATATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2503 CCAAAGTATGG
XhoII 1857 AGATCT [GA]^GATC[CT]
2675 AGATCC
XmaCI 32 CCCGGG C^CCGGG
100 CCCGGG
XmaI 32 CCCGGG C^CCGGG
100 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BcnI BfaI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsmI BssKI BstAPI BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I