Restriction map for
pF1KA1106
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# NO CUT ENZYMES ( 74 enzymes) #
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AatII AclI AflII Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AviII AvrII BbeI
BcgI BclI BfrI BglII BlnI
BlpI Bpu1102I BsiWI BsmBI Bsp1407I
BspDI BspLU11I BsrDI BsrGI BssHII
Bsu36I CelII ClaI DraI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoRV FbaI FseI FspI HpaI
KasI MfeI MunI NaeI NarI
NgoMIV NheI NotI NruI PacI
PmeI PshAI PshBI Psp1406I SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SwaI XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 2801 GGTACC G^GTACC
AcyI 954 GACGCC G[GA]^CG[CT]C
AgeI 2255 ACCGGT A^CCGGT
AhdI 703 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2801 GGTACC G^GTACC
AspEI 703 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 504 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3258 GGATCC G^GATCC
BbrPI 1977 CACGTG CAC^GTG
BciVI 2403 GTATCC GTATCC(6/5)
BfaI 2389 CTAG C^TAG
BsaBI 3325 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 954 GACGCC G[GA]^CG[CT]C
BsaI 871 GAGACC GGTCTC(1/5)
BsmI 2510 GCATTC GAATGC(1/-1)
BspHI 1059 TCATGA T^CATGA
BspMI 210 ACCTGC ACCTGC(4/8)
BsrBI 1063 GAGCGG CCGCTC(-3/-3)
BssSI 3193 CACGAG CACGAG(-5/-1)
Bst1107I 3312 GTATAC GTA^TAC
BstBI 2513 TTCGAA TT^CGAA
BstEII 2415 GGTGACC G^GT[ACGT]ACC
BstPI 2415 GGTGACC G^GT[ACGT]ACC
BstYI 3258 GGATCC [GA]^GATC[CT]
BstZ17I 3312 GTATAC GTA^TAC
CpoI 1640 CGGACCG CG^G[AT]CCG
DrdI 1294 GACAGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 703 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1737 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2415 GGTGACC G^GT[ACGT]ACC
EcoRI 3386 GAATTC G^AATTC
EcoT22I 1465 ATGCAT ATGCA^T
Hin1I 954 GACGCC G[GA]^CG[CT]C
HincII 1519 GTCAAC GT[CT]^[GA]AC
HindII 1519 GTCAAC GT[CT]^[GA]AC
HindIII 2962 AAGCTT A^AGCTT
KpnI 2801 GGTACC GGTAC^C
KspI 2182 CCGCGG CCGC^GG
MaeI 2389 CTAG C^TAG
MamI 3325 GATCGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3258 GGATCC [GA]^GATC[CT]
MluI 2744 ACGCGT A^CGCGT
NcoI 1202 CCATGG C^CATGG
NdeI 1235 CATATG CA^TATG
NsiI 1465 ATGCAT ATGCA^T
NspV 2513 TTCGAA TT^CGAA
PaeR7I 1750 CTCGAG C^TCGAG
PflFI 504 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2255 ACCGGT A^CCGGT
PmaCI 1977 CACGTG CAC^GTG
PmlI 1977 CACGTG CAC^GTG
PstI 1568 CTGCAG CTGCA^G
PvuI 1562 CGATCG CGAT^CG
RcaI 1059 TCATGA T^CATGA
RsrII 1640 CGGACCG CG^G[AT]CCG
SacI 1747 GAGCTC GAGCT^C
SacII 2182 CCGCGG CCGC^GG
SfuI 2513 TTCGAA TT^CGAA
SspI 1757 AATATT AAT^ATT
StuI 1634 AGGCCT AGG^CCT
Tth111I 504 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2388 TCTAGA T^CTAGA
XcmI 3071 CCAATTCCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1750 CTCGAG C^TCGAG
XhoII 3258 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccI 2299 GTAGAC GT^[AC][GT]AC
3312 GTATAC
AccIII 1420 TCCGGA T^CCGGA
1552 TCCGGA
AcsI 3276 AAATTT [GA]^AATT[CT]
3386 GAATTC
AflIII 1616 ACGTGT A^C[GA][CT]GT
2744 ACGCGT
ApoI 3276 AAATTT [GA]^AATT[CT]
3386 GAATTC
BanI 2768 GGTGCC G^G[CT][GA]CC
2801 GGTACC
BbvCI 2160 CCTCAGC CCTCAGC(-5/-2)
478 GCTGAGG
BglI 490 GCCTACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2023 GCCCACATGGC
BseAI 1420 TCCGGA T^CCGGA
1552 TCCGGA
BsgI 1500 CTGCAC GTGCAG(16/14)
1980 GTGCAG
BsiEI 1018 CGGTCG CG[GA][CT]^CG
1562 CGATCG
BspEI 1420 TCCGGA T^CCGGA
1552 TCCGGA
BstAPI 1973 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2849 GCATGTCCTGC
HaeII 1966 AGCGCC [GA]GCGC^[CT]
2758 AGCGCC
HgaI 954 GACGC GACGC(5/10)
2746 GCGTC
MroI 1420 TCCGGA T^CCGGA
1552 TCCGGA
PflMI 3192 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3225 CCACAGCCTGG
SfcI 1568 CTGCAG C^T[GA][CT]AG
1698 CTACAG
Van91I 3192 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3225 CCACAGCCTGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaAI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmaCI XmaI