Restriction map for
pF1KA1109
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# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccII AccIII AgeI AocI
Aor51HI AscI Asp718I AspI AviII
AvrII BbeI BbrPI BcgI BlnI
BsaAI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BssHII Bst1107I
BstUI BstZ17I Bsu36I ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
Eco81I FseI FspI HaeII HpaI
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NgoMIV NotI
NruI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacII SbfI SfiI SfoI SgrAI
SmaI SnaBI Sse8387I Tth111I XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 6866 GACGTC GACGT^C
AflIII 2257 ACATGT A^C[GA][CT]GT
ApaI 8066 GGGCCC GGGCC^C
AvaI 4940 CTCGAG C^[CT]CG[GA]G
BalI 5344 TGGCCA TGG^CCA
BclI 8142 TGATCA T^GATCA
BglI 6404 GCCCTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI 5288 CGTCTC CGTCTC(1/5)
BsoBI 4940 CTCGAG C^[CT]CG[GA]G
BspLU11I 2257 ACATGT A^CATGT
BspMI 1294 GCAGGT ACCTGC(4/8)
BssSI 7541 CTCGTG CACGAG(-5/-1)
BstAPI 4597 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2771 TTCGAA TT^CGAA
BstEII 8199 GGTTACC G^GT[ACGT]ACC
BstPI 8199 GGTTACC G^GT[ACGT]ACC
CfoI 6807 GCGC GCG^C
EcoNI 6568 CCTGGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 8199 GGTTACC G^GT[ACGT]ACC
FauI 4824 GCGGG CCCGC(4/6)
FbaI 8142 TGATCA T^GATCA
HhaI 6807 GCGC GCG^C
HinP1I 6807 GCGC G^CGC
MfeI 3839 CAATTG C^AATTG
MluNI 5344 TGGCCA TGG^CCA
MscI 5344 TGGCCA TGG^CCA
MunI 3839 CAATTG C^AATTG
NspV 2771 TTCGAA TT^CGAA
PaeR7I 4940 CTCGAG C^TCGAG
PpuMI 7222 GGGACCC [GA]G^G[AT]CC[CT]
SalI 6419 GTCGAC G^TCGAC
ScaI 4141 AGTACT AGT^ACT
SfuI 2771 TTCGAA TT^CGAA
StuI 4010 AGGCCT AGG^CCT
SwaI 3668 ATTTAAAT ATTT^AAAT
XhoI 4940 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 35 enzymes) #
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AccI 6419 GTCGAC GT^[AC][GT]AC
7806 GTCTAC
AclI 4745 AACGTT AA^CGTT
7273 AACGTT
AcyI 6866 GACGTC G[GA]^CG[CT]C
8258 GGCGTC
Alw44I 1620 GTGCAC G^TGCAC
3071 GTGCAC
ApaLI 1620 GTGCAC G^TGCAC
3071 GTGCAC
AseI 1569 ATTAAT AT^TAAT
8835 ATTAAT
AsnI 1569 ATTAAT AT^TAAT
8835 ATTAAT
BanI 4300 GGCACC G^G[CT][GA]CC
6691 GGTGCC
BcnI 4615 CCGGG CC^[GC]GG
5662 CCCGG
BlpI 529 GCTCAGC GC^T[ACGT]AGC
8608 GCTGAGC
Bpu1102I 529 GCTCAGC GC^T[ACGT]AGC
8608 GCTGAGC
BsaBI 3667 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3727 GATTTTCATC
BsaHI 6866 GACGTC G[GA]^CG[CT]C
8258 GGCGTC
BsaI 4100 GAGACC GGTCTC(1/5)
6881 GAGACC
BsrDI 4491 CATTGC GCAATG(2/0)
5945 CATTGC
BsrFI 695 ACCGGC [GA]^CCGG[CT]
5593 ACCGGC
BstXI 4008 CCAGGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6835 CCAAGAGCATGG
CelII 529 GCTCAGC GC^T[ACGT]AGC
8608 GCTGAGC
Cfr10I 695 ACCGGC [GA]^CCGG[CT]
5593 ACCGGC
DraII 7222 GGGACCC [GA]G^G[ACGT]CC[CT]
8066 GGGCCCT
DrdI 1154 GACACAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8705 GACAAAAAAGTC
EcoO109I 7222 GGGACCC [GA]G^G[ACGT]CC[CT]
8066 GGGCCCT
EcoRI 1998 GAATTC G^AATTC
8397 GAATTC
EcoRV 4657 GATATC GAT^ATC
7713 GATATC
HgaI 5697 GACGC GACGC(5/10)
8259 GCGTC
Hin1I 6866 GACGTC G[GA]^CG[CT]C
8258 GGCGTC
MamI 3667 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3727 GATTTTCATC
NciI 4615 CCGGG CC^[GC]GG
5662 CCCGG
NheI 4606 GCTAGC G^CTAGC
6364 GCTAGC
PshBI 1569 ATTAAT AT^TAAT
8835 ATTAAT
Psp1406I 4745 AACGTT AA^CGTT
7273 AACGTT
PstI 5876 CTGCAG CTGCA^G
6308 CTGCAG
SpeI 6114 ACTAGT A^CTAGT
7321 ACTAGT
SphI 4156 GCATGC GCATG^C
6487 GCATGC
XbaI 1906 TCTAGA T^CTAGA
8817 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AflII AhdI
AluI AlwI AlwNI ApoI Asp700I
AspEI AspHI AvaII BamHI BanII
BbsI BbvCI BbvI BciVI BfaI
BfrI BglII BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DsaI EaeI
Eam1105I EarI EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI