Restriction map for
pF1KA1111
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AviII AvrII
BbrPI BbvCI BcgI BclI BfrI
BglI BlnI BsaAI BsaBI BsgI
BsiWI BsmBI BspDI BsrDI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRV
EcoT22I FbaI FspI HincII HindII
HpaI KspI MamI MfeI MunI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacII SalI SapI SfiI
SgrAI SnaBI SpeI SspI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 875 GGTACC G^GTACC
AccI 1894 GTAGAC GT^[AC][GT]AC
AccIII 380 TCCGGA T^CCGGA
AclI 1447 AACGTT AA^CGTT
ApaI 280 GGGCCC GGGCC^C
Asp700I 409 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 875 GGTACC G^GTACC
AvaI 2699 CCCGGG C^[CT]CG[GA]G
BamHI 1058 GGATCC G^GATCC
BbeI 3053 GGCGCC GGCGC^C
BciVI 3143 GTATCC GTATCC(6/5)
BlpI 1164 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1164 GCTGAGC GC^T[ACGT]AGC
BsaWI 380 TCCGGA [AT]^CCGG[AT]
BseAI 380 TCCGGA T^CCGGA
BsmI 2177 GCATTC GAATGC(1/-1)
BsoBI 2699 CCCGGG C^[CT]CG[GA]G
Bsp1407I 798 TGTACA T^GTACA
BspEI 380 TCCGGA T^CCGGA
BspLU11I 179 ACATGT A^CATGT
BsrBI 2972 CCGCTC CCGCTC(-3/-3)
BsrGI 798 TGTACA T^GTACA
BstBI 737 TTCGAA TT^CGAA
CelII 1164 GCTGAGC GC^T[ACGT]AGC
DraIII 1050 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
FseI 75 GGCCGGCC GGCCGG^CC
KasI 3053 GGCGCC G^GCGCC
KpnI 875 GGTACC GGTAC^C
MluI 33 ACGCGT A^CGCGT
MroI 380 TCCGGA T^CCGGA
NarI 3053 GGCGCC GG^CGCC
NspV 737 TTCGAA TT^CGAA
Psp1406I 1447 AACGTT AA^CGTT
PvuII 2735 CAGCTG CAG^CTG
SbfI 2334 CCTGCAGG CCTGCA^GG
ScaI 806 AGTACT AGT^ACT
SfoI 3053 GGCGCC GGC^GCC
SfuI 737 TTCGAA TT^CGAA
SmaI 2699 CCCGGG CCC^GGG
SphI 2279 GCATGC GCATG^C
Sse8387I 2334 CCTGCAGG CCTGCA^GG
SspBI 798 TGTACA T^GTACA
XmaCI 2699 CCCGGG C^CCGGG
XmaI 2699 CCCGGG C^CCGGG
XmnI 409 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 1074 GACGCC G[GA]^CG[CT]C
3053 GGCGCC
AflIII 33 ACGCGT A^C[GA][CT]GT
179 ACATGT
AlwNI 1699 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
2732 CAGCAGCTG
BfaI 1904 CTAG C^TAG
2330 CTAG
BglII 899 AGATCT A^GATCT
1401 AGATCT
BsaHI 1074 GACGCC G[GA]^CG[CT]C
3053 GGCGCC
BsaI 789 GAGACC GGTCTC(1/5)
1201 GAGACC
BsiEI 78 CGGCCG CG[GA][CT]^CG
3138 CGGCCG
BspHI 164 TCATGA T^CATGA
815 TCATGA
BspMI 2297 ACCTGC ACCTGC(4/8)
2205 GCAGGT
BstAPI 2279 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2731 GCAGCAGCTGC
EagI 78 CGGCCG C^GGCCG
3138 CGGCCG
EclXI 78 CGGCCG C^GGCCG
3138 CGGCCG
Eco52I 78 CGGCCG C^GGCCG
3138 CGGCCG
HaeII 2167 GGCGCT [GA]GCGC^[CT]
3053 GGCGCC
Hin1I 1074 GACGCC G[GA]^CG[CT]C
3053 GGCGCC
HindIII 619 AAGCTT A^AGCTT
2149 AAGCTT
MaeI 1904 CTAG C^TAG
2330 CTAG
MaeII 364 ACGT A^CGT
1448 ACGT
MseI 1121 TTAA T^TAA
1397 TTAA
NcoI 1290 CCATGG C^CATGG
2632 CCATGG
NspI 179 ACATGT [GA]CATG^[CT]
2279 GCATGC
PflMI 1049 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1205 CCATCTGTTGG
PpuMI 2214 GGGACCT [GA]G^G[AT]CC[CT]
2695 GGGACCC
RcaI 164 TCATGA T^CATGA
815 TCATGA
SacI 376 GAGCTC GAGCT^C
2376 GAGCTC
SexAI 992 ACCAGGT A^CC[AT]GGT
1283 ACCTGGT
StuI 1587 AGGCCT AGG^CCT
2339 AGGCCT
Tru9I 1121 TTAA T^TAA
1397 TTAA
Van91I 1049 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1205 CCATCTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII