Restriction map for
pF1KA1115
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# NO CUT ENZYMES ( 88 enzymes) #
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Acc65I AccI AclI AcsI AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BciVI BclI
BfaI BglII BlnI BsiWI BsmI
BspDI BspHI BssHII Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MaeI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 1332 GACGTC GACGT^C
AccIII 1985 TCCGGA T^CCGGA
AflII 753 CTTAAG C^TTAAG
AflIII 761 ACATGT A^C[GA][CT]GT
Asp700I 864 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 1069 CACGTG CAC^GTG
BcgI 758 GCAACATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 753 CTTAAG C^TTAAG
BsaAI 1069 CACGTG [CT]AC^GT[GA]
BsaBI 610 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1985 TCCGGA [AT]^CCGG[AT]
BseAI 1985 TCCGGA T^CCGGA
Bsp1407I 1356 TGTACA T^GTACA
BspEI 1985 TCCGGA T^CCGGA
BspLU11I 761 ACATGT A^CATGT
BsrDI 166 GCAATG GCAATG(2/0)
BsrGI 1356 TGTACA T^GTACA
BstXI 1971 CCAGCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 1976 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1375 CCTCGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 118 GTCAAC GT[CT]^[GA]AC
HindII 118 GTCAAC GT[CT]^[GA]AC
KspI 964 CCGCGG CCGC^GG
MamI 610 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 549 CAATTG C^AATTG
MroI 1985 TCCGGA T^CCGGA
MseI 754 TTAA T^TAA
MslI 510 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 549 CAATTG C^AATTG
PmaCI 1069 CACGTG CAC^GTG
PmlI 1069 CACGTG CAC^GTG
SacII 964 CCGCGG CCGC^GG
SspBI 1356 TGTACA T^GTACA
Tru9I 754 TTAA T^TAA
Tsp509I 550 AATT ^AATT
XmnI 864 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 34 enzymes) #
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AccII 965 CGCG CG^CG
1061 CGCG
AfaI 228 GTAC GT^AC
1357 GTAC
AocI 1630 CCTGAGG CC^T[ACGT]AGG
2335 CCTCAGG
AvaI 73 CCCGAG C^[CT]CG[GA]G
2180 CCCGAG
BbeI 943 GGCGCC GGCGC^C
1927 GGCGCC
BlpI 969 GCTCAGC GC^T[ACGT]AGC
1230 GCTGAGC
Bpu1102I 969 GCTCAGC GC^T[ACGT]AGC
1230 GCTGAGC
BsiEI 1350 CGACCG CG[GA][CT]^CG
1605 CGACCG
BsmBI 736 GAGACG CGTCTC(1/5)
1264 GAGACG
BsoBI 73 CCCGAG C^[CT]CG[GA]G
2180 CCCGAG
BssSI 1123 CACGAG CACGAG(-5/-1)
415 CTCGTG
BstEII 180 GGTCACC G^GT[ACGT]ACC
1393 GGTCACC
BstPI 180 GGTCACC G^GT[ACGT]ACC
1393 GGTCACC
BstUI 965 CGCG CG^CG
1061 CGCG
Bsu36I 1630 CCTGAGG CC^T[ACGT]AGG
2335 CCTCAGG
CelII 969 GCTCAGC GC^T[ACGT]AGC
1230 GCTGAGC
Eco81I 1630 CCTGAGG CC^T[ACGT]AGG
2335 CCTCAGG
EcoO65I 180 GGTCACC G^GT[ACGT]ACC
1393 GGTCACC
KasI 943 GGCGCC G^GCGCC
1927 GGCGCC
MvnI 965 CGCG CG^CG
1061 CGCG
NarI 943 GGCGCC GG^CGCC
1927 GGCGCC
PflMI 708 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2385 CCAGGCCTTGG
RsaI 228 GTAC GT^AC
1357 GTAC
SacI 898 GAGCTC GAGCT^C
1126 GAGCTC
SapI 2197 GAAGAGC GCTCTTC(1/4)
2532 GAAGAGC
SexAI 1559 ACCTGGT A^CC[AT]GGT
1977 ACCTGGT
SfiI 906 GGCCCAAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1950 GGCCAGGGGGGCC
SfoI 943 GGCGCC GGC^GCC
1927 GGCGCC
SphI 1034 GCATGC GCATG^C
1197 GCATGC
StuI 1652 AGGCCT AGG^CCT
2387 AGGCCT
TaqI 604 TCGA T^CGA
767 TCGA
TfiI 249 GATTC G^A[AT]TC
615 GAATC
TthHB8I 604 TCGA T^CGA
767 TCGA
Van91I 708 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2385 CCAGGCCTTGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
ApaI AspHI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TseI Tsp45I TspRI XcmI XhoII