Restriction map for
pF1KA1231
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BamHI BbeI BciVI
BfrI BlnI BsaBI BsaI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I Bsu36I ClaI
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRV FseI HindIII KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI SacII SbfI
ScaI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 947 GTCGAC GT^[AC][GT]AC
Alw44I 1677 GTGCAC G^TGCAC
ApaLI 1677 GTGCAC G^TGCAC
AvaI 347 CCCGGG C^[CT]CG[GA]G
BbrPI 1642 CACGTG CAC^GTG
BclI 2366 TGATCA T^GATCA
BglI 2725 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1667 AGATCT A^GATCT
BseRI 268 GAGGAG GAGGAG(10/8)
BsmBI 574 GAGACG CGTCTC(1/5)
BsoBI 347 CCCGGG C^[CT]CG[GA]G
BsrBI 1866 CCGCTC CCGCTC(-3/-3)
BstBI 2300 TTCGAA TT^CGAA
BstXI 2694 CCAGGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 1742 CGGACCG CG^G[AT]CCG
DraI 1330 TTTAAA TTT^AAA
DraII 453 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 453 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1822 ATGCAT ATGCA^T
FbaI 2366 TGATCA T^GATCA
HaeII 2401 AGCGCC [GA]GCGC^[CT]
HpaI 2022 GTTAAC GTT^AAC
NcoI 1395 CCATGG C^CATGG
NsiI 1822 ATGCAT ATGCA^T
NspV 2300 TTCGAA TT^CGAA
PflMI 2849 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1642 CACGTG CAC^GTG
PmlI 1642 CACGTG CAC^GTG
RsrII 1742 CGGACCG CG^G[AT]CCG
SacI 2137 GAGCTC GAGCT^C
SalI 947 GTCGAC G^TCGAC
SapI 1291 GCTCTTC GCTCTTC(1/4)
SfuI 2300 TTCGAA TT^CGAA
SmaI 347 CCCGGG CCC^GGG
SmlI 499 CTCAAG C^T[CT][GA]AG
Van91I 2849 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 347 CCCGGG C^CCGGG
XmaI 347 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 282 CGCG CG^CG
1957 CGCG
AcyI 959 GGCGTC G[GA]^CG[CT]C
2538 GGCGTC
AflIII 1641 ACACGT A^C[GA][CT]GT
2837 ACACGT
AviII 1250 TGCGCA TGC^GCA
1646 TGCGCA
BcgI 2426 CGACGGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3060 CGAGCCACCTGC
BfaI 121 CTAG C^TAG
883 CTAG
BsaAI 1642 CACGTG [CT]AC^GT[GA]
2838 CACGTA
BsaHI 959 GGCGTC G[GA]^CG[CT]C
2538 GGCGTC
BspHI 753 TCATGA T^CATGA
870 TCATGA
BsrDI 41 CATTGC GCAATG(2/0)
2446 GCAATG
BsrFI 332 ACCGGC [GA]^CCGG[CT]
336 GCCGGT
BstUI 282 CGCG CG^CG
1957 CGCG
BstYI 207 GGATCT [GA]^GATC[CT]
1667 AGATCT
Cfr10I 332 ACCGGC [GA]^CCGG[CT]
336 GCCGGT
DrdI 2165 GACCCGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2451 GACAGAACTGTC
EagI 334 CGGCCG C^GGCCG
952 CGGCCG
EclXI 334 CGGCCG C^GGCCG
952 CGGCCG
Eco52I 334 CGGCCG C^GGCCG
952 CGGCCG
EcoT14I 1395 CCATGG C^C[AT][AT]GG
1463 CCTTGG
FspI 1250 TGCGCA TGC^GCA
1646 TGCGCA
Hin1I 959 GGCGTC G[GA]^CG[CT]C
2538 GGCGTC
HincII 947 GTCGAC GT[CT]^[GA]AC
2022 GTTAAC
HindII 947 GTCGAC GT[CT]^[GA]AC
2022 GTTAAC
MaeI 121 CTAG C^TAG
883 CTAG
MflI 207 GGATCT [GA]^GATC[CT]
1667 AGATCT
MvnI 282 CGCG CG^CG
1957 CGCG
RcaI 753 TCATGA T^CATGA
870 TCATGA
SexAI 56 ACCAGGT A^CC[AT]GGT
632 ACCTGGT
SphI 285 GCATGC GCATG^C
1961 GCATGC
StyI 1395 CCATGG C^C[AT][AT]GG
1463 CCTTGG
XcmI 250 CCACACGACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
674 CCATAAAAAATGTGG
XhoII 207 GGATCT [GA]^GATC[CT]
1667 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraIII
DsaI EaeI EarI EcoRI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI