Restriction map for
pF1KA1351
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI Asp718I
AspEI AspI AvaII AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BsrFI BssSI Bst1107I BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FseI FspI HaeII
KasI KpnI KspI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SnaBI Sse8387I SspI
StuI Tth111I XbaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 914 GTCTAC GT^[AC][GT]AC
AcyI 3232 GACGCC G[GA]^CG[CT]C
Alw44I 1348 GTGCAC G^TGCAC
ApaI 50 GGGCCC GGGCC^C
ApaLI 1348 GTGCAC G^TGCAC
AseI 2295 ATTAAT AT^TAAT
AsnI 2295 ATTAAT AT^TAAT
Asp700I 2565 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 50 GGGCCC G[GA]GC[CT]^C
BglII 952 AGATCT A^GATCT
BpmI 1774 CTGGAG CTGGAG(16/14)
BsaAI 941 TACGTG [CT]AC^GT[GA]
BsaHI 3232 GACGCC G[GA]^CG[CT]C
BsmBI 3424 GAGACG CGTCTC(1/5)
BspHI 1139 TCATGA T^CATGA
BspLU11I 776 ACATGT A^CATGT
BssHII 34 GCGCGC G^CGCGC
BstAPI 3541 GCAGATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 947 TTCGAA TT^CGAA
BstEII 1853 GGTCACC G^GT[ACGT]ACC
BstPI 1853 GGTCACC G^GT[ACGT]ACC
DraI 2193 TTTAAA TTT^AAA
DsaI 1910 CCATGG C^C[GA][CT]GG
EcoO65I 1853 GGTCACC G^GT[ACGT]ACC
Hin1I 3232 GACGCC G[GA]^CG[CT]C
HindIII 3473 AAGCTT A^AGCTT
HpaI 2508 GTTAAC GTT^AAC
MfeI 1304 CAATTG C^AATTG
MspA1I 3614 CAGCTG C[AC]G^C[GT]G
MunI 1304 CAATTG C^AATTG
NcoI 1910 CCATGG C^CATGG
NspV 947 TTCGAA TT^CGAA
PshAI 1923 GACCGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2295 ATTAAT AT^TAAT
PvuII 3614 CAGCTG CAG^CTG
RcaI 1139 TCATGA T^CATGA
SfuI 947 TTCGAA TT^CGAA
SmaI 2024 CCCGGG CCC^GGG
SpeI 1579 ACTAGT A^CTAGT
SphI 333 GCATGC GCATG^C
SwaI 2192 ATTTAAAT ATTT^AAAT
XmaCI 2024 CCCGGG C^CCGGG
XmaI 2024 CCCGGG C^CCGGG
XmnI 2565 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 35 CGCG CG^CG
385 CGCG
AflIII 776 ACATGT A^C[GA][CT]GT
942 ACGTGT
AocI 581 CCTCAGG CC^T[ACGT]AGG
3304 CCTGAGG
BalI 644 TGGCCA TGG^CCA
2055 TGGCCA
BciVI 2331 GGATAC GTATCC(6/5)
2221 GTATCC
BclI 1967 TGATCA T^GATCA
2982 TGATCA
BsaBI 801 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
994 GATCCAGATC
BsmI 432 GAATGC GAATGC(1/-1)
1104 GAATGC
Bsp1407I 2306 TGTACA T^GTACA
2998 TGTACA
BsrGI 2306 TGTACA T^GTACA
2998 TGTACA
BstUI 35 CGCG CG^CG
385 CGCG
BstXI 225 CCATAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3146 CCATTAAGTTGG
Bsu36I 581 CCTCAGG CC^T[ACGT]AGG
3304 CCTGAGG
CfoI 34 GCGC GCG^C
36 GCGC
EaeI 644 TGGCCA [CT]^GGCC[GA]
2055 TGGCCA
Eco81I 581 CCTCAGG CC^T[ACGT]AGG
3304 CCTGAGG
EcoNI 577 CCTCCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2275 CCTGGTAAAGG
FbaI 1967 TGATCA T^GATCA
2982 TGATCA
HgaI 3232 GACGC GACGC(5/10)
3426 GACGC
HhaI 34 GCGC GCG^C
36 GCGC
HinP1I 34 GCGC G^CGC
36 GCGC
MamI 801 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
994 GATCCAGATC
MluNI 644 TGGCCA TGG^CCA
2055 TGGCCA
MscI 644 TGGCCA TGG^CCA
2055 TGGCCA
MvnI 35 CGCG CG^CG
385 CGCG
NspI 333 GCATGC [GA]CATG^[CT]
776 ACATGT
PflMI 449 CCAAACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2113 CCACCAGATGG
PleI 2456 GAGTC GAGTC(4/5)
3643 GAGTC
SapI 462 GAAGAGC GCTCTTC(1/4)
1872 GAAGAGC
SspBI 2306 TGTACA T^GTACA
2998 TGTACA
Van91I 449 CCAAACTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2113 CCACCAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI BanI
BbvI BcnI BfaI BmyI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PaeR7I
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoI XhoII