Restriction map for
pF1KA1448
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# NO CUT ENZYMES ( 83 enzymes) #
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Acc65I AgeI Alw44I AocI ApaI
ApaLI AscI Asp718I AspI AviII
BbrPI BbvCI BclI BsaBI BsgI
BsiEI BsiWI BsmBI BsmI BspDI
BspHI BspLU11I BspMI BsrFI BssHII
Bst1107I BstBI BstZ17I Bsu36I Cfr10I
ClaI CpoI EagI EclXI Eco52I
Eco81I EcoNI EcoRI FbaI FseI
FspI KpnI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PstI PvuI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SpeI
SphI Sse8387I StuI SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 1257 GTCTAC GT^[AC][GT]AC
AccIII 1610 TCCGGA T^CCGGA
AclI 1286 AACGTT AA^CGTT
AflII 2538 CTTAAG C^TTAAG
AflIII 2706 ACGTGT A^C[GA][CT]GT
AhdI 2008 GACAAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1664 AGCGCT AGC^GCT
Asp700I 3149 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2008 GACAAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 50 CTCGAG C^[CT]CG[GA]G
AvrII 1428 CCTAGG C^CTAGG
BbeI 1556 GGCGCC GGCGC^C
BcgI 2817 GCAGCTTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2379 GGATAC GTATCC(6/5)
BfrI 2538 CTTAAG C^TTAAG
BglI 341 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1428 CCTAGG C^CTAGG
BsaAI 1678 TACGTA [CT]AC^GT[GA]
BseAI 1610 TCCGGA T^CCGGA
BsoBI 50 CTCGAG C^[CT]CG[GA]G
BspEI 1610 TCCGGA T^CCGGA
BssSI 1781 CACGAG CACGAG(-5/-1)
BstEII 3232 GGTCACC G^GT[ACGT]ACC
BstPI 3232 GGTCACC G^GT[ACGT]ACC
DraI 1290 TTTAAA TTT^AAA
DraIII 652 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2744 GACGTGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2008 GACAAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1664 AGCGCT AGC^GCT
EcoO65I 3232 GGTCACC G^GT[ACGT]ACC
EcoRV 985 GATATC GAT^ATC
EcoT22I 75 ATGCAT ATGCA^T
HgaI 1978 GACGC GACGC(5/10)
HincII 1465 GTTAAC GT[CT]^[GA]AC
HindII 1465 GTTAAC GT[CT]^[GA]AC
HindIII 1342 AAGCTT A^AGCTT
HpaI 1465 GTTAAC GTT^AAC
KasI 1556 GGCGCC G^GCGCC
MroI 1610 TCCGGA T^CCGGA
NarI 1556 GGCGCC GG^CGCC
NsiI 75 ATGCAT ATGCA^T
PaeR7I 50 CTCGAG C^TCGAG
Psp1406I 1286 AACGTT AA^CGTT
SapI 1594 GAAGAGC GCTCTTC(1/4)
SfoI 1556 GGCGCC GGC^GCC
SnaBI 1678 TACGTA TAC^GTA
SspI 808 AATATT AAT^ATT
XhoI 50 CTCGAG C^TCGAG
XmnI 3149 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AseI 811 ATTAAT AT^TAAT
3112 ATTAAT
AsnI 811 ATTAAT AT^TAAT
3112 ATTAAT
AspHI 1008 GAGCAC G[AT]GC[AT]^C
1133 GTGCTC
BamHI 2730 GGATCC G^GATCC
3042 GGATCC
BanI 775 GGTGCC G^G[CT][GA]CC
1556 GGCGCC
BcnI 30 CCGGG CC^[GC]GG
1770 CCCGG
BfaI 745 CTAG C^TAG
1429 CTAG
BsaWI 1610 TCCGGA [AT]^CCGG[AT]
2864 ACCGGA
BsiHKAI 1008 GAGCAC G[AT]GC[AT]^C
1133 GTGCTC
Bsp1407I 218 TGTACA T^GTACA
3249 TGTACA
BsrGI 218 TGTACA T^GTACA
3249 TGTACA
BstAPI 958 GCAATGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1024 GCAGATCGTGC
BstXI 605 CCAGGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1361 CCATCAGAATGG
MaeI 745 CTAG C^TAG
1429 CTAG
NciI 30 CCGGG CC^[GC]GG
1770 CCCGG
NcoI 1166 CCATGG C^CATGG
3055 CCATGG
PflMI 514 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2107 CCAAGCAGTGG
PshBI 811 ATTAAT AT^TAAT
3112 ATTAAT
PvuII 1711 CAGCTG CAG^CTG
2716 CAGCTG
SspBI 218 TGTACA T^GTACA
3249 TGTACA
Van91I 514 CCACTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2107 CCAAGCAGTGG
XcmI 1073 CCAATGCCAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2715 CCAGCTGATGAATGG
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# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AvaII BalI BanII BbsI BbvI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PpuMI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII