Restriction map for
pF1KA1450
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BlnI BsaAI BsaBI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
Bst1107I BstXI BstZ17I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco52I
EcoRV FseI FspI KasI KpnI
MamI MluI MroI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflMI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 57 CGCG CG^CG
AcyI 63 GGCGTC G[GA]^CG[CT]C
AflIII 407 ACATGT A^C[GA][CT]GT
AgeI 2087 ACCGGT A^CCGGT
AocI 85 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1244 AGCGCT AGC^GCT
ApaI 2580 GGGCCC GGGCC^C
BalI 1424 TGGCCA TGG^CCA
BclI 746 TGATCA T^GATCA
BglII 2730 AGATCT A^GATCT
BlpI 2436 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2436 GCTCAGC GC^T[ACGT]AGC
BsaHI 63 GGCGTC G[GA]^CG[CT]C
BsaWI 2087 ACCGGT [AT]^CCGG[AT]
BsmI 1935 GAATGC GAATGC(1/-1)
BspLU11I 407 ACATGT A^CATGT
BsrDI 714 CATTGC GCAATG(2/0)
BstAPI 2419 GCAGATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 812 TTCGAA TT^CGAA
BstEII 1630 GGTGACC G^GT[ACGT]ACC
BstPI 1630 GGTGACC G^GT[ACGT]ACC
BstUI 57 CGCG CG^CG
Bsu36I 85 CCTAAGG CC^T[ACGT]AGG
CelII 2436 GCTCAGC GC^T[ACGT]AGC
DrdI 2754 GACTGGAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1244 AGCGCT AGC^GCT
Eco81I 85 CCTAAGG CC^T[ACGT]AGG
EcoNI 85 CCTAAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1630 GGTGACC G^GT[ACGT]ACC
EcoRI 1141 GAATTC G^AATTC
FbaI 746 TGATCA T^GATCA
Hin1I 63 GGCGTC G[GA]^CG[CT]C
HpaI 1323 GTTAAC GTT^AAC
KspI 56 CCGCGG CCGC^GG
MfeI 1049 CAATTG C^AATTG
MluNI 1424 TGGCCA TGG^CCA
MscI 1424 TGGCCA TGG^CCA
MunI 1049 CAATTG C^AATTG
MvnI 57 CGCG CG^CG
NspV 812 TTCGAA TT^CGAA
PinAI 2087 ACCGGT A^CCGGT
PshAI 1825 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 56 CCGCGG CCGC^GG
ScaI 3307 AGTACT AGT^ACT
SfuI 812 TTCGAA TT^CGAA
SmlI 1252 CTCAAG C^T[CT][GA]AG
StuI 2228 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 981 GAATTT [GA]^AATT[CT]
1141 GAATTC
ApoI 981 GAATTT [GA]^AATT[CT]
1141 GAATTC
Asp700I 21 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
361 GAACAGCTTC
AspI 1534 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2929 GACCTTGTC
BanI 1895 GGTGCC G^G[CT][GA]CC
1968 GGCACC
BbsI 697 GAAGAC GAAGAC(2/6)
511 GTCTTC
BcnI 2124 CCGGG CC^[GC]GG
3219 CCGGG
BglI 1802 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2251 GCCAGTGAGGC
BpuAI 697 GAAGAC GAAGAC(2/6)
511 GTCTTC
BsgI 2474 GTGCAG GTGCAG(16/14)
3016 GTGCAG
BsrBI 1727 CCGCTC CCGCTC(-3/-3)
2832 CCGCTC
BsrFI 2087 ACCGGT [GA]^CCGG[CT]
2890 ACCGGC
BssSI 3230 CACGAG CACGAG(-5/-1)
2590 CTCGTG
Cfr10I 2087 ACCGGT [GA]^CCGG[CT]
2890 ACCGGC
DraI 1641 TTTAAA TTT^AAA
2302 TTTAAA
DsaI 56 CCGCGG C^C[GA][CT]GG
2469 CCACGG
EcoT22I 1937 ATGCAT ATGCA^T
3157 ATGCAT
HgaI 1915 GACGC GACGC(5/10)
64 GCGTC
HindIII 2166 AAGCTT A^AGCTT
3127 AAGCTT
MaeII 480 ACGT A^CGT
1398 ACGT
NciI 2124 CCGGG CC^[GC]GG
3219 CCGGG
NsiI 1937 ATGCAT ATGCA^T
3157 ATGCAT
PflFI 1534 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2929 GACCTTGTC
SapI 24 GCTCTTC GCTCTTC(1/4)
1982 GCTCTTC
TaqI 720 TCGA T^CGA
813 TCGA
Tth111I 1534 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2929 GACCTTGTC
TthHB8I 720 TCGA T^CGA
813 TCGA
XmnI 21 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
361 GAACAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanII BbvI BfaI BmyI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII