Restriction map for
pF1KA1457
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# NO CUT ENZYMES ( 69 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AvrII BamHI BclI
BfrI BlnI BseAI BsiWI BspDI
BspEI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
Eco47III EcoRI EcoRV EcoT22I FbaI
HindIII HpaI KpnI MfeI MluI
MroI MseI MunI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tru9I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AcsI 316 AAATTC [GA]^AATT[CT]
AhdI 1789 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3125 CCTCAGG CC^T[ACGT]AGG
ApoI 316 AAATTC [GA]^AATT[CT]
AspEI 1789 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1550 TGGCCA TGG^CCA
BcgI 3293 GCACAGAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3659 AGATCT A^GATCT
BlpI 708 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 708 GCTGAGC GC^T[ACGT]AGC
BsrDI 869 GCAATG GCAATG(2/0)
BstAPI 1699 GCATTTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3125 CCTCAGG CC^T[ACGT]AGG
CelII 708 GCTGAGC GC^T[ACGT]AGC
Eam1105I 1789 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3125 CCTCAGG CC^T[ACGT]AGG
EcoNI 228 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MluNI 1550 TGGCCA TGG^CCA
MscI 1550 TGGCCA TGG^CCA
NcoI 3495 CCATGG C^CATGG
NdeI 98 CATATG CA^TATG
NheI 3334 GCTAGC G^CTAGC
PstI 3691 CTGCAG CTGCA^G
SalI 1936 GTCGAC G^TCGAC
SapI 81 GAAGAGC GCTCTTC(1/4)
SexAI 289 ACCAGGT A^CC[AT]GGT
SfiI 3817 GGCCTGCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tsp509I 317 AATT ^AATT
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# TWO CUT ENZYMES ( 36 enzymes) #
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AvaI 1800 CCCGGG C^[CT]CG[GA]G
2532 CCCGGG
BbeI 1312 GGCGCC GGCGC^C
2590 GGCGCC
BbrPI 1267 CACGTG CAC^GTG
2965 CACGTG
BbvCI 1473 CCTCAGC CCTCAGC(-5/-2)
2070 GCTGAGG
BciVI 2019 GGATAC GTATCC(6/5)
3147 GGATAC
BfaI 883 CTAG C^TAG
3335 CTAG
BsaBI 2649 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3660 GATCTACATC
BsaWI 640 ACCGGA [AT]^CCGG[AT]
2996 ACCGGA
BsmBI 825 CGTCTC CGTCTC(1/5)
929 CGTCTC
BsmI 267 GAATGC GAATGC(1/-1)
1815 GAATGC
BsoBI 1800 CCCGGG C^[CT]CG[GA]G
2532 CCCGGG
Bsp1407I 65 TGTACA T^GTACA
3629 TGTACA
BspHI 2864 TCATGA T^CATGA
3110 TCATGA
BsrGI 65 TGTACA T^GTACA
3629 TGTACA
BssSI 1045 CACGAG CACGAG(-5/-1)
3904 CACGAG
BstEII 3075 GGTCACC G^GT[ACGT]ACC
3156 GGTGACC
BstPI 3075 GGTCACC G^GT[ACGT]ACC
3156 GGTGACC
DraIII 178 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1089 CACCAAGTG
EcoO65I 3075 GGTCACC G^GT[ACGT]ACC
3156 GGTGACC
KasI 1312 GGCGCC G^GCGCC
2590 GGCGCC
KspI 2282 CCGCGG CCGC^GG
4016 CCGCGG
MaeI 883 CTAG C^TAG
3335 CTAG
MamI 2649 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3660 GATCTACATC
NarI 1312 GGCGCC GG^CGCC
2590 GGCGCC
PmaCI 1267 CACGTG CAC^GTG
2965 CACGTG
PmlI 1267 CACGTG CAC^GTG
2965 CACGTG
RcaI 2864 TCATGA T^CATGA
3110 TCATGA
SacII 2282 CCGCGG CCGC^GG
4016 CCGCGG
SfoI 1312 GGCGCC GGC^GCC
2590 GGCGCC
SmaI 1800 CCCGGG CCC^GGG
2532 CCCGGG
SmlI 898 CTCAAG C^T[CT][GA]AG
2202 CTTGAG
SspBI 65 TGTACA T^GTACA
3629 TGTACA
StuI 677 AGGCCT AGG^CCT
2039 AGGCCT
TfiI 21 GATTC G^A[AT]TC
1593 GATTC
XmaCI 1800 CCCGGG C^CCGGG
2532 CCCGGG
XmaI 1800 CCCGGG C^CCGGG
2532 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI AspHI AspI AvaII
AviII BanI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FseI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SphI
StyI TaqI TseI Tsp45I TspRI
Tth111I TthHB8I Van91I XcmI XhoII