Restriction map for
pF1KA1516
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# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AclI AhdI Aor51HI ApaI
AscI Asp718I AspEI AspI AviII
AvrII BlnI BsiEI BsiWI BspLU11I
BsrBI BssHII BstEII BstPI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FseI FspI HpaI
KpnI MluI NaeI NdeI NgoMIV
NheI NotI NruI PacI PflFI
PmeI Psp1406I PvuI RsrII SbfI
SexAI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 2321 TCCGGA T^CCGGA
AflII 6697 CTTAAG C^TTAAG
AflIII 2074 ACACGT A^C[GA][CT]GT
AgeI 1399 ACCGGT A^CCGGT
Alw44I 2569 GTGCAC G^TGCAC
AocI 1950 CCTCAGG CC^T[ACGT]AGG
ApaLI 2569 GTGCAC G^TGCAC
BbeI 2155 GGCGCC GGCGC^C
BbrPI 2075 CACGTG CAC^GTG
BfrI 6697 CTTAAG C^TTAAG
BlpI 2743 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2743 GCTGAGC GC^T[ACGT]AGC
BsaBI 4338 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2321 TCCGGA T^CCGGA
BsmBI 4328 GAGACG CGTCTC(1/5)
Bsp1407I 374 TGTACA T^GTACA
BspEI 2321 TCCGGA T^CCGGA
BsrFI 1399 ACCGGT [GA]^CCGG[CT]
BsrGI 374 TGTACA T^GTACA
BssSI 3997 CTCGTG CACGAG(-5/-1)
BstBI 5788 TTCGAA TT^CGAA
Bsu36I 1950 CCTCAGG CC^T[ACGT]AGG
CelII 2743 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1399 ACCGGT [GA]^CCGG[CT]
Eco81I 1950 CCTCAGG CC^T[ACGT]AGG
EcoT22I 5051 ATGCAT ATGCA^T
HaeII 2155 GGCGCC [GA]GCGC^[CT]
KasI 2155 GGCGCC G^GCGCC
KspI 2182 CCGCGG CCGC^GG
MamI 4338 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2321 TCCGGA T^CCGGA
NarI 2155 GGCGCC GG^CGCC
NsiI 5051 ATGCAT ATGCA^T
NspV 5788 TTCGAA TT^CGAA
PaeR7I 6725 CTCGAG C^TCGAG
PinAI 1399 ACCGGT A^CCGGT
PmaCI 2075 CACGTG CAC^GTG
PmlI 2075 CACGTG CAC^GTG
PshAI 4088 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2182 CCGCGG CCGC^GG
ScaI 1453 AGTACT AGT^ACT
SfoI 2155 GGCGCC GGC^GCC
SfuI 5788 TTCGAA TT^CGAA
SphI 5693 GCATGC GCATG^C
SspBI 374 TGTACA T^GTACA
StuI 2510 AGGCCT AGG^CCT
SwaI 5471 ATTTAAAT ATTT^AAAT
XhoI 6725 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 3489 GACGTC GACGT^C
5683 GACGTC
AseI 3376 ATTAAT AT^TAAT
6094 ATTAAT
AsnI 3376 ATTAAT AT^TAAT
6094 ATTAAT
AvaI 6725 CTCGAG C^[CT]CG[GA]G
6797 CCCGAG
BamHI 1465 GGATCC G^GATCC
5598 GGATCC
BsaAI 2075 CACGTG [CT]AC^GT[GA]
2545 TACGTG
BsoBI 6725 CTCGAG C^[CT]CG[GA]G
6797 CCCGAG
BspDI 1567 ATCGAT AT^CGAT
4449 ATCGAT
BspHI 2168 TCATGA T^CATGA
3272 TCATGA
Bst1107I 1020 GTATAC GTA^TAC
2830 GTATAC
BstZ17I 1020 GTATAC GTA^TAC
2830 GTATAC
CfoI 1361 GCGC GCG^C
2156 GCGC
ClaI 1567 ATCGAT AT^CGAT
4449 ATCGAT
EcoNI 2580 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3087 CCTTTATCAGG
HhaI 1361 GCGC GCG^C
2156 GCGC
HinP1I 1361 GCGC G^CGC
2156 GCGC
MfeI 1037 CAATTG C^AATTG
3776 CAATTG
MunI 1037 CAATTG C^AATTG
3776 CAATTG
PshBI 3376 ATTAAT AT^TAAT
6094 ATTAAT
RcaI 2168 TCATGA T^CATGA
3272 TCATGA
SalI 1657 GTCGAC G^TCGAC
5680 GTCGAC
SapI 3440 GAAGAGC GCTCTTC(1/4)
2991 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BglI
BglII BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI EcoO109I EcoRI EcoRII
EcoRV EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI