Restriction map for
pF1KA1675
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
Aor51HI ApoI AscI AseI AsnI
Asp718I AspI AviII AvrII BamHI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsmBI BsmI Bsp1407I BspDI
BspHI BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I CelII ClaI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MfeI MluI MunI NcoI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI SacII SalI SbfI
SexAI SfiI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AatII 1816 GACGTC GACGT^C
AccIII 2993 TCCGGA T^CCGGA
AhdI 3421 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 622 GTGCAC G^TGCAC
ApaLI 622 GTGCAC G^TGCAC
AspEI 3421 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BsaBI 475 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2993 TCCGGA T^CCGGA
BsiWI 200 CGTACG C^GTACG
BspEI 2993 TCCGGA T^CCGGA
BspLU11I 1790 ACATGT A^CATGT
CpoI 2222 CGGACCG CG^G[AT]CCG
DraI 3660 TTTAAA TTT^AAA
DraIII 549 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3147 GACCAGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3421 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 1819 GTCAAC GT[CT]^[GA]AC
HindII 1819 GTCAAC GT[CT]^[GA]AC
MamI 475 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2993 TCCGGA T^CCGGA
NheI 27 GCTAGC G^CTAGC
PflMI 274 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 934 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2764 CTGCAG CTGCA^G
RsrII 2222 CGGACCG CG^G[AT]CCG
SapI 2676 GCTCTTC GCTCTTC(1/4)
TfiI 3649 GATTC G^A[AT]TC
Tsp509I 3637 AATT ^AATT
Van91I 274 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 33 enzymes) #
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AccI 214 GTCTAC GT^[AC][GT]AC
640 GTCTAC
AlwI 5 GATCC GGATC(4/5)
237 GATCC
AocI 2829 CCTCAGG CC^T[ACGT]AGG
2907 CCTGAGG
BalI 86 TGGCCA TGG^CCA
1284 TGGCCA
BfaI 28 CTAG C^TAG
3328 CTAG
BglII 437 AGATCT A^GATCT
2873 AGATCT
BsaI 2704 GAGACC GGTCTC(1/5)
1674 GGTCTC
BsaWI 2795 TCCGGT [AT]^CCGG[AT]
2993 TCCGGA
BsgI 2856 GTGCAG GTGCAG(16/14)
3675 GTGCAG
BsrDI 2094 CATTGC GCAATG(2/0)
3703 GCAATG
BstBI 1009 TTCGAA TT^CGAA
2973 TTCGAA
BstYI 437 AGATCT [GA]^GATC[CT]
2873 AGATCT
Bsu36I 2829 CCTCAGG CC^T[ACGT]AGG
2907 CCTGAGG
EagI 1573 CGGCCG C^GGCCG
2662 CGGCCG
EclXI 1573 CGGCCG C^GGCCG
2662 CGGCCG
Eco52I 1573 CGGCCG C^GGCCG
2662 CGGCCG
Eco81I 2829 CCTCAGG CC^T[ACGT]AGG
2907 CCTGAGG
MaeI 28 CTAG C^TAG
3328 CTAG
MflI 437 AGATCT [GA]^GATC[CT]
2873 AGATCT
MluNI 86 TGGCCA TGG^CCA
1284 TGGCCA
MscI 86 TGGCCA TGG^CCA
1284 TGGCCA
NaeI 1229 GCCGGC GCC^GGC
2503 GCCGGC
NdeI 1182 CATATG CA^TATG
3698 CATATG
NgoMIV 1229 GCCGGC G^CCGGC
2503 GCCGGC
NspI 1790 ACATGT [GA]CATG^[CT]
3290 ACATGC
NspV 1009 TTCGAA TT^CGAA
2973 TTCGAA
PpuMI 77 GGGACCT [GA]G^G[AT]CC[CT]
3125 GGGTCCT
SacI 253 GAGCTC GAGCT^C
799 GAGCTC
SfcI 2598 CTACAG C^T[GA][CT]AG
2764 CTGCAG
SfuI 1009 TTCGAA TT^CGAA
2973 TTCGAA
SgrAI 1321 CACCGGCG C[GA]^CCGG[CT]G
2502 CGCCGGCG
StuI 947 AGGCCT AGG^CCT
3324 AGGCCT
XhoII 437 AGATCT [GA]^GATC[CT]
2873 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI ApaI Asp700I AspHI
AvaI AvaII BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BcnI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI PmaCI PmlI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfoI SmaI SmlI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
XcmI XmaCI XmaI XmnI