Restriction map for
pF1KA1738
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbeI BcgI
BglI BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I CelII ClaI
CpoI EcoNI EcoRI EcoT22I FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NarI NdeI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 73 enzymes) #
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AccI 585 GTCGAC GT^[AC][GT]AC
AccII 1347 CGCG CG^CG
AcsI 2736 GAATTT [GA]^AATT[CT]
AcyI 875 GACGCC G[GA]^CG[CT]C
AflII 1734 CTTAAG C^TTAAG
AflIII 3162 ACATGT A^C[GA][CT]GT
AhdI 1930 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 67 GTGCAC G^TGCAC
AlwNI 2918 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2020 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2854 AGCGCT AGC^GCT
ApaI 424 GGGCCC GGGCC^C
ApaLI 67 GTGCAC G^TGCAC
ApoI 2736 GAATTT [GA]^AATT[CT]
Asp700I 1076 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1930 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 243 TGGCCA TGG^CCA
BanI 2216 GGCACC G^G[CT][GA]CC
BbrPI 70 CACGTG CAC^GTG
BbvCI 1984 GCTGAGG CCTCAGC(-5/-2)
BciVI 690 GGATAC GTATCC(6/5)
BfrI 1734 CTTAAG C^TTAAG
BsaAI 70 CACGTG [CT]AC^GT[GA]
BsaHI 875 GACGCC G[GA]^CG[CT]C
BsaI 2725 GAGACC GGTCTC(1/5)
BsmBI 324 CGTCTC CGTCTC(1/5)
BspLU11I 3162 ACATGT A^CATGT
BspMI 2342 GCAGGT ACCTGC(4/8)
BsrFI 256 GCCGGC [GA]^CCGG[CT]
BstAPI 2307 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1800 GGTCACC G^GT[ACGT]ACC
BstPI 1800 GGTCACC G^GT[ACGT]ACC
BstUI 1347 CGCG CG^CG
Bsu36I 2020 CCTCAGG CC^T[ACGT]AGG
CfoI 2855 GCGC GCG^C
Cfr10I 256 GCCGGC [GA]^CCGG[CT]
DraI 832 TTTAAA TTT^AAA
DrdI 2198 GACACAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1391 CGGCCG C^GGCCG
Eam1105I 1930 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1391 CGGCCG C^GGCCG
Eco47III 2854 AGCGCT AGC^GCT
Eco52I 1391 CGGCCG C^GGCCG
Eco81I 2020 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1800 GGTCACC G^GT[ACGT]ACC
EcoRV 1618 GATATC GAT^ATC
HaeII 2854 AGCGCT [GA]GCGC^[CT]
HgaI 875 GACGC GACGC(5/10)
HhaI 2855 GCGC GCG^C
Hin1I 875 GACGCC G[GA]^CG[CT]C
HinP1I 2855 GCGC G^CGC
MluNI 243 TGGCCA TGG^CCA
MscI 243 TGGCCA TGG^CCA
MvnI 1347 CGCG CG^CG
NaeI 256 GCCGGC GCC^GGC
NcoI 3317 CCATGG C^CATGG
NgoMIV 256 GCCGGC G^CCGGC
NheI 433 GCTAGC G^CTAGC
PaeR7I 2722 CTCGAG C^TCGAG
PflMI 3184 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 70 CACGTG CAC^GTG
PmlI 70 CACGTG CAC^GTG
PshAI 645 GACATTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2590 GAGCTC GAGCT^C
SalI 585 GTCGAC G^TCGAC
ScaI 161 AGTACT AGT^ACT
SmaI 2813 CCCGGG CCC^GGG
Van91I 3184 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1476 CCACTATGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2722 CTCGAG C^TCGAG
XmaCI 2813 CCCGGG C^CCGGG
XmaI 2813 CCCGGG C^CCGGG
XmnI 1076 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 1672 GATCC GGATC(4/5)
2544 GATCC
BclI 2661 TGATCA T^GATCA
3372 TGATCA
BglII 1609 AGATCT A^GATCT
2035 AGATCT
BsiEI 587 CGACCG CG[GA][CT]^CG
1391 CGGCCG
BsmI 2472 GAATGC GAATGC(1/-1)
2818 GCATTC
Bsp1407I 1262 TGTACA T^GTACA
2333 TGTACA
BspHI 563 TCATGA T^CATGA
1517 TCATGA
BsrGI 1262 TGTACA T^GTACA
2333 TGTACA
DraIII 1477 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
1553 CACCAAGTG
DsaI 665 CCACGG C^C[GA][CT]GG
3317 CCATGG
FbaI 2661 TGATCA T^GATCA
3372 TGATCA
HincII 585 GTCGAC GT[CT]^[GA]AC
3106 GTCAAC
HindII 585 GTCGAC GT[CT]^[GA]AC
3106 GTCAAC
NspI 41 ACATGC [GA]CATG^[CT]
3162 ACATGT
PpuMI 1997 AGGACCC [GA]G^G[AT]CC[CT]
2704 GGGTCCT
PstI 1523 CTGCAG CTGCA^G
2386 CTGCAG
PvuII 1982 CAGCTG CAG^CTG
2411 CAGCTG
RcaI 563 TCATGA T^CATGA
1517 TCATGA
SspBI 1262 TGTACA T^GTACA
2333 TGTACA
SspI 2490 AATATT AAT^ATT
2993 AATATT
StuI 1179 AGGCCT AGG^CCT
2642 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII