Restriction map for
pF1KA1788
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
DpnII DraI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI Ksp632I KspI
MaeI MamI MboI MfeI MflI
MluI MroI MslI MunI NdeII
NheI NotI NruI NsiI PacI
PflFI PinAI PmeI PshBI Psp1406I
PstI PvuI RcaI RsaI SacII
SapI Sau3AI SbfI ScaI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp509I Tth111I XbaI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 282 GTCGAC GT^[AC][GT]AC
AflIII 719 ACGTGT A^C[GA][CT]GT
AlwNI 211 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 690 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 740 TGGCCA TGG^CCA
BbeI 379 GGCGCC GGCGC^C
BbrPI 1072 CACGTG CAC^GTG
BbvCI 1116 CCTCAGC CCTCAGC(-5/-2)
BglI 1127 GCCTCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1072 CACGTG [CT]AC^GT[GA]
BsaWI 650 ACCGGA [AT]^CCGG[AT]
BseRI 679 GAGGAG GAGGAG(10/8)
BsgI 772 GTGCAG GTGCAG(16/14)
BsmBI 18 CGTCTC CGTCTC(1/5)
BsmI 3 GAATGC GAATGC(1/-1)
BsrFI 36 GCCGGC [GA]^CCGG[CT]
BsrI 949 CCAGT ACTGG(1/-1)
BstBI 842 TTCGAA TT^CGAA
BstF5I 521 GGATG GGATG(2/0)
Cfr10I 36 GCCGGC [GA]^CCGG[CT]
CpoI 1157 CGGACCG CG^G[AT]CCG
DsaI 47 CCATGG C^C[GA][CT]GG
EcoNI 756 CCTCACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 521 GGATG GGATG(9/13)
HincII 282 GTCGAC GT[CT]^[GA]AC
HindII 282 GTCGAC GT[CT]^[GA]AC
KasI 379 GGCGCC G^GCGCC
MluNI 740 TGGCCA TGG^CCA
MscI 740 TGGCCA TGG^CCA
MseI 260 TTAA T^TAA
NaeI 36 GCCGGC GCC^GGC
NarI 379 GGCGCC GG^CGCC
NcoI 47 CCATGG C^CATGG
NdeI 863 CATATG CA^TATG
NgoMIV 36 GCCGGC G^CCGGC
NspI 992 GCATGT [GA]CATG^[CT]
NspV 842 TTCGAA TT^CGAA
PaeR7I 1170 CTCGAG C^TCGAG
PflMI 1217 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1072 CACGTG CAC^GTG
PmlI 1072 CACGTG CAC^GTG
PpuMI 212 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 12 GACTTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 1157 CGGACCG CG^G[AT]CCG
SalI 282 GTCGAC G^TCGAC
SfcI 60 CTACAG C^T[GA][CT]AG
SfoI 379 GGCGCC GGC^GCC
SfuI 842 TTCGAA TT^CGAA
SmlI 1170 CTCGAG C^T[CT][GA]AG
StuI 1125 AGGCCT AGG^CCT
Tru9I 260 TTAA T^TAA
Van91I 1217 CCATTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 665 CCAGCTACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1170 CTCGAG C^TCGAG
XmnI 690 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 188 GGGCCC GGGCC^C
565 GGGCCC
AvaI 884 CCCGAG C^[CT]CG[GA]G
1170 CTCGAG
BanI 379 GGCGCC G^G[CT][GA]CC
981 GGTGCC
BbsI 702 GAAGAC GAAGAC(2/6)
694 GTCTTC
BcgI 376 CGAGGCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
834 GCAGCAGGTTCG
BpuAI 702 GAAGAC GAAGAC(2/6)
694 GTCTTC
BsiEI 38 CGGCCG CG[GA][CT]^CG
146 CGGCCG
BsmAI 10 GAGAC GTCTC(1/5)
19 GTCTC
BsoBI 884 CCCGAG C^[CT]CG[GA]G
1170 CTCGAG
BstXI 596 CCAGCCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1008 CCACCCCCCTGG
EagI 38 CGGCCG C^GGCCG
146 CGGCCG
EclXI 38 CGGCCG C^GGCCG
146 CGGCCG
Eco52I 38 CGGCCG C^GGCCG
146 CGGCCG
EcoT14I 47 CCATGG C^C[AT][AT]GG
1199 CCAAGG
HaeII 199 GGCGCT [GA]GCGC^[CT]
379 GGCGCC
MaeII 719 ACGT A^CGT
1073 ACGT
MboII 702 GAAGA GAAGA(8/7)
695 TCTTC
PvuII 673 CAGCTG CAG^CTG
736 CAGCTG
SacI 474 GAGCTC GAGCT^C
1141 GAGCTC
StyI 47 CCATGG C^C[AT][AT]GG
1199 CCAAGG
TfiI 167 GATTC G^A[AT]TC
906 GATTC
Tsp45I 1036 GTCAC ^GT[GC]AC
1070 GTCAC
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaII BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeIII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PleI Sau96I ScrFI
SfaNI TaqI TseI TspRI TthHB8I