Restriction map for
pF1KA1793
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# NO CUT ENZYMES ( 88 enzymes) #
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Acc65I AccI AccII AclI AcsI
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BlnI BsaAI BsiEI
BsiWI Bsp1407I BspDI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoRI EcoRV FseI FspI HindIII
HpaI KasI KpnI KspI MfeI
MluI MunI MvnI NarI NdeI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 1575 TCCGGA T^CCGGA
AflII 406 CTTAAG C^TTAAG
Aor51HI 2540 AGCGCT AGC^GCT
ApaI 722 GGGCCC GGGCC^C
Asp700I 556 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3453 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2654 TGGCCA TGG^CCA
BcgI 2511 CGAGGAACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2570 TGATCA T^GATCA
BfrI 406 CTTAAG C^TTAAG
BglII 765 AGATCT A^GATCT
BsaBI 579 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1575 TCCGGA T^CCGGA
BspEI 1575 TCCGGA T^CCGGA
BspHI 1211 TCATGA T^CATGA
BstEII 2994 GGTGACC G^GT[ACGT]ACC
BstPI 2994 GGTGACC G^GT[ACGT]ACC
DsaI 2735 CCATGG C^C[GA][CT]GG
EaeI 2654 TGGCCA [CT]^GGCC[GA]
Eco47III 2540 AGCGCT AGC^GCT
EcoO65I 2994 GGTGACC G^GT[ACGT]ACC
EcoT22I 928 ATGCAT ATGCA^T
FbaI 2570 TGATCA T^GATCA
HincII 133 GTCAAC GT[CT]^[GA]AC
HindII 133 GTCAAC GT[CT]^[GA]AC
MamI 579 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2654 TGGCCA TGG^CCA
MroI 1575 TCCGGA T^CCGGA
MscI 2654 TGGCCA TGG^CCA
NaeI 1259 GCCGGC GCC^GGC
NcoI 2735 CCATGG C^CATGG
NgoMIV 1259 GCCGGC G^CCGGC
NsiI 928 ATGCAT ATGCA^T
PflFI 3453 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1777 CCAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1520 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2077 GACGCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1211 TCATGA T^CATGA
SphI 1859 GCATGC GCATG^C
TaqI 2019 TCGA T^CGA
Tth111I 3453 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2019 TCGA T^CGA
Van91I 1777 CCAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 556 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AatII 831 GACGTC GACGT^C
3453 GACGTC
AflIII 1297 ACATGT A^C[GA][CT]GT
1499 ACATGT
AocI 1377 CCTCAGG CC^T[ACGT]AGG
3205 CCTGAGG
AvaI 224 CCCGGG C^[CT]CG[GA]G
2933 CCCGGG
AvaII 1521 GGACC G^G[AT]CC
2253 GGTCC
BciVI 1383 GGATAC GTATCC(6/5)
3012 GGATAC
BsaWI 631 TCCGGT [AT]^CCGG[AT]
1575 TCCGGA
BsmBI 1426 GAGACG CGTCTC(1/5)
3451 GAGACG
BsoBI 224 CCCGGG C^[CT]CG[GA]G
2933 CCCGGG
BspLU11I 1297 ACATGT A^CATGT
1499 ACATGT
BsrBI 1486 GAGCGG CCGCTC(-3/-3)
2134 GAGCGG
BsrDI 1409 GCAATG GCAATG(2/0)
2261 GCAATG
BsrFI 1259 GCCGGC [GA]^CCGG[CT]
2080 GCCGGT
Bsu36I 1377 CCTCAGG CC^T[ACGT]AGG
3205 CCTGAGG
CfoI 299 GCGC GCG^C
2541 GCGC
Cfr10I 1259 GCCGGC [GA]^CCGG[CT]
2080 GCCGGT
Eco81I 1377 CCTCAGG CC^T[ACGT]AGG
3205 CCTGAGG
HaeII 298 AGCGCC [GA]GCGC^[CT]
2540 AGCGCT
HhaI 299 GCGC GCG^C
2541 GCGC
HinP1I 299 GCGC G^CGC
2541 GCGC
NheI 291 GCTAGC G^CTAGC
2812 GCTAGC
SmaI 224 CCCGGG CCC^GGG
2933 CCCGGG
XcmI 2033 CCAAGCTGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3038 CCAGAAAGAGTCTGG
XmaCI 224 CCCGGG C^CCGGG
2933 CCCGGG
XmaI 224 CCCGGG C^CCGGG
2933 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CelII Cfr13I
DdeI DpnII DraII EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII