Restriction map for
pF1KA1927
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI AocI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvrII BamHI BbrPI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrGI BssSI
BstEII BstPI BstXI Bsu36I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRV FseI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MroI MunI NheI NotI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI SexAI SfiI SgrAI SmaI
SpeI Sse8387I SspBI SspI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Alw44I 1343 GTGCAC G^TGCAC
Aor51HI 3163 AGCGCT AGC^GCT
ApaLI 1343 GTGCAC G^TGCAC
AviII 3107 TGCGCA TGC^GCA
BanI 2448 GGCGCC G^G[CT][GA]CC
BbeI 2448 GGCGCC GGCGC^C
BbsI 1651 GAAGAC GAAGAC(2/6)
BbvCI 3619 GCTGAGG CCTCAGC(-5/-2)
BclI 3003 TGATCA T^GATCA
BglI 3074 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1651 GAAGAC GAAGAC(2/6)
BsaAI 1496 TACGTA [CT]AC^GT[GA]
BsgI 3496 GTGCAG GTGCAG(16/14)
BsmBI 112 GAGACG CGTCTC(1/5)
BspHI 1203 TCATGA T^CATGA
BsrBI 3126 CCGCTC CCGCTC(-3/-3)
BsrDI 2941 GCAATG GCAATG(2/0)
BssHII 182 GCGCGC G^CGCGC
Bst1107I 1968 GTATAC GTA^TAC
BstAPI 1747 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1399 TTCGAA TT^CGAA
BstZ17I 1968 GTATAC GTA^TAC
DraIII 1643 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3163 AGCGCT AGC^GCT
EcoRI 462 GAATTC G^AATTC
EcoT22I 3628 ATGCAT ATGCA^T
FbaI 3003 TGATCA T^GATCA
FspI 3107 TGCGCA TGC^GCA
KasI 2448 GGCGCC G^GCGCC
KspI 2452 CCGCGG CCGC^GG
NaeI 192 GCCGGC GCC^GGC
NarI 2448 GGCGCC GG^CGCC
NdeI 2974 CATATG CA^TATG
NgoMIV 192 GCCGGC G^CCGGC
NruI 233 TCGCGA TCG^CGA
NsiI 3628 ATGCAT ATGCA^T
NspV 1399 TTCGAA TT^CGAA
RcaI 1203 TCATGA T^CATGA
SacII 2452 CCGCGG CCGC^GG
ScaI 2830 AGTACT AGT^ACT
SfoI 2448 GGCGCC GGC^GCC
SfuI 1399 TTCGAA TT^CGAA
SnaBI 1496 TACGTA TAC^GTA
SphI 3110 GCATGC GCATG^C
StuI 74 AGGCCT AGG^CCT
XbaI 3481 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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AccI 1968 GTATAC GT^[AC][GT]AC
2523 GTCTAC
AcyI 786 GACGCC G[GA]^CG[CT]C
2448 GGCGCC
BalI 416 TGGCCA TGG^CCA
746 TGGCCA
BcnI 178 CCCGG CC^[GC]GG
782 CCGGG
BsaHI 786 GACGCC G[GA]^CG[CT]C
2448 GGCGCC
BsaI 2287 GAGACC GGTCTC(1/5)
2207 GGTCTC
BsrFI 5 ACCGGC [GA]^CCGG[CT]
192 GCCGGC
Cfr10I 5 ACCGGC [GA]^CCGG[CT]
192 GCCGGC
EaeI 416 TGGCCA [CT]^GGCC[GA]
746 TGGCCA
Hin1I 786 GACGCC G[GA]^CG[CT]C
2448 GGCGCC
MluNI 416 TGGCCA TGG^CCA
746 TGGCCA
MscI 416 TGGCCA TGG^CCA
746 TGGCCA
NciI 178 CCCGG CC^[GC]GG
782 CCGGG
NcoI 2060 CCATGG C^CATGG
2960 CCATGG
NspI 2676 ACATGC [GA]CATG^[CT]
3110 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanII BbvI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI