Restriction map for
pF1KA1938
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# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AclI AflII Aor51HI
AscI AseI AsnI Asp700I AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BlnI BsaAI BsaBI BseAI
BsiWI Bsp1407I BspEI BsrGI BssHII
Bst1107I BstEII BstPI BstZ17I CpoI
DraI Eco47III EcoO65I EcoRV EcoT22I
FbaI FseI HpaI KspI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI PacI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SgrAI
SnaBI Sse8387I SspBI SspI SwaI
XbaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 1085 GGTACC G^GTACC
AcsI 1534 GAATTC [GA]^AATT[CT]
AflIII 3629 ACATGT A^C[GA][CT]GT
AgeI 1421 ACCGGT A^CCGGT
AocI 3137 CCTCAGG CC^T[ACGT]AGG
ApaI 509 GGGCCC GGGCC^C
ApoI 1534 GAATTC [GA]^AATT[CT]
Asp718I 1085 GGTACC G^GTACC
AviII 1726 TGCGCA TGC^GCA
BbeI 1064 GGCGCC GGCGC^C
BbsI 916 GTCTTC GAAGAC(2/6)
BglII 1997 AGATCT A^GATCT
BpuAI 916 GTCTTC GAAGAC(2/6)
BsmBI 246 GAGACG CGTCTC(1/5)
BsmI 3408 GCATTC GAATGC(1/-1)
BspDI 3555 ATCGAT AT^CGAT
BspLU11I 3629 ACATGT A^CATGT
BssSI 2463 CACGAG CACGAG(-5/-1)
BstBI 2580 TTCGAA TT^CGAA
Bsu36I 3137 CCTCAGG CC^T[ACGT]AGG
ClaI 3555 ATCGAT AT^CGAT
DraIII 1672 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1496 GACTGATTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3137 CCTCAGG CC^T[ACGT]AGG
EcoRI 1534 GAATTC G^AATTC
FspI 1726 TGCGCA TGC^GCA
HindIII 525 AAGCTT A^AGCTT
KasI 1064 GGCGCC G^GCGCC
KpnI 1085 GGTACC GGTAC^C
NarI 1064 GGCGCC GG^CGCC
NspV 2580 TTCGAA TT^CGAA
PinAI 1421 ACCGGT A^CCGGT
ScaI 3545 AGTACT AGT^ACT
SfoI 1064 GGCGCC GGC^GCC
SfuI 2580 TTCGAA TT^CGAA
SmaI 812 CCCGGG CCC^GGG
SpeI 1350 ACTAGT A^CTAGT
Tsp509I 1535 AATT ^AATT
XmaCI 812 CCCGGG C^CCGGG
XmaI 812 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AcyI 1064 GGCGCC G[GA]^CG[CT]C
2666 GACGCC
AhdI 248 GACGCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2302 GACCTTCAGTC
AspEI 248 GACGCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2302 GACCTTCAGTC
AspI 910 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2740 GACGGTGTC
BfaI 566 CTAG C^TAG
1351 CTAG
BglI 372 GCCCTTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1051 GCCACCCTGGC
BlpI 2106 GCTCAGC GC^T[ACGT]AGC
3841 GCTGAGC
Bpu1102I 2106 GCTCAGC GC^T[ACGT]AGC
3841 GCTGAGC
BsaHI 1064 GGCGCC G[GA]^CG[CT]C
2666 GACGCC
BsaI 8 GGTCTC GGTCTC(1/5)
3471 GGTCTC
BsiEI 707 CGGCCG CG[GA][CT]^CG
2591 CGGCCG
BspHI 546 TCATGA T^CATGA
1943 TCATGA
BsrDI 1875 CATTGC GCAATG(2/0)
3200 CATTGC
BstAPI 2493 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3084 GCAGCACATGC
BstXI 2676 CCAGGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2895 CCACCGAGGTGG
CelII 2106 GCTCAGC GC^T[ACGT]AGC
3841 GCTGAGC
EagI 707 CGGCCG C^GGCCG
2591 CGGCCG
Eam1105I 248 GACGCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2302 GACCTTCAGTC
EclXI 707 CGGCCG C^GGCCG
2591 CGGCCG
Eco52I 707 CGGCCG C^GGCCG
2591 CGGCCG
HaeII 1064 GGCGCC [GA]GCGC^[CT]
1768 AGCGCC
Hin1I 1064 GGCGCC G[GA]^CG[CT]C
2666 GACGCC
MaeI 566 CTAG C^TAG
1351 CTAG
MaeII 101 ACGT A^CGT
1212 ACGT
MseI 530 TTAA T^TAA
941 TTAA
NaeI 951 GCCGGC GCC^GGC
3569 GCCGGC
NgoMIV 951 GCCGGC G^CCGGC
3569 GCCGGC
PflFI 910 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2740 GACGGTGTC
PvuII 163 CAGCTG CAG^CTG
575 CAGCTG
RcaI 546 TCATGA T^CATGA
1943 TCATGA
SphI 2076 GCATGC GCATG^C
2708 GCATGC
StuI 2325 AGGCCT AGG^CCT
2621 AGGCCT
Tru9I 530 TTAA T^TAA
941 TTAA
Tth111I 910 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2740 GACGGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
Alw44I AlwI AlwNI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BmyI BpmI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PaeR7I PflMI
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoI XhoII