Restriction map for
pF1KA2001
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrGI
BssHII Bst1107I BstAPI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MroI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfiI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 1634 GACGTC GACGT^C
AccI 481 GTCTAC GT^[AC][GT]AC
ApaI 346 GGGCCC GGGCC^C
AspHI 859 GAGCTC G[AT]GC[AT]^C
AspI 475 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1080 GGACC G^G[AT]CC
BalI 166 TGGCCA TGG^CCA
BamHI 1320 GGATCC G^GATCC
BbeI 550 GGCGCC GGCGC^C
BbrPI 701 CACGTG CAC^GTG
BglI 1550 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 701 CACGTG [CT]AC^GT[GA]
BsiHKAI 859 GAGCTC G[AT]GC[AT]^C
BsmI 84 GAATGC GAATGC(1/-1)
BsrDI 143 GCAATG GCAATG(2/0)
BssSI 1688 CTCGTG CACGAG(-5/-1)
BstXI 1437 CCAGACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1320 GGATCC [GA]^GATC[CT]
HincII 870 GTCAAC GT[CT]^[GA]AC
HindII 870 GTCAAC GT[CT]^[GA]AC
KasI 550 GGCGCC G^GCGCC
MflI 1320 GGATCC [GA]^GATC[CT]
MluI 1427 ACGCGT A^CGCGT
MluNI 166 TGGCCA TGG^CCA
MscI 166 TGGCCA TGG^CCA
NarI 550 GGCGCC GG^CGCC
NspI 639 GCATGC [GA]CATG^[CT]
PflFI 475 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 596 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 701 CACGTG CAC^GTG
PmlI 701 CACGTG CAC^GTG
SacI 859 GAGCTC GAGCT^C
ScaI 1025 AGTACT AGT^ACT
SfoI 550 GGCGCC GGC^GCC
SgrAI 383 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 730 CTCAAG C^T[CT][GA]AG
SphI 639 GCATGC GCATG^C
Tsp45I 616 GTCAC ^GT[GC]AC
Tth111I 475 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 596 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1320 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 702 ACGTGT A^C[GA][CT]GT
1427 ACGCGT
AlwNI 775 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1325 CAGGGACTG
Asp700I 229 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1546 GAAGGCCTTC
BbsI 1042 GAAGAC GAAGAC(2/6)
1631 GAAGAC
BpuAI 1042 GAAGAC GAAGAC(2/6)
1631 GAAGAC
BsrFI 263 GCCGGC [GA]^CCGG[CT]
384 GCCGGC
BstBI 965 TTCGAA TT^CGAA
1628 TTCGAA
BstEII 615 GGTCACC G^GT[ACGT]ACC
1459 GGTTACC
BstPI 615 GGTCACC G^GT[ACGT]ACC
1459 GGTTACC
Cfr10I 263 GCCGGC [GA]^CCGG[CT]
384 GCCGGC
EcoNI 117 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
189 CCTTGCGGAGG
EcoO65I 615 GGTCACC G^GT[ACGT]ACC
1459 GGTTACC
EcoT14I 596 CCAAGG C^C[AT][AT]GG
1076 CCAAGG
HaeII 387 GGCGCT [GA]GCGC^[CT]
550 GGCGCC
MaeIII 616 GTCAC ^GT[ACGT]AC
1460 GTTAC
NaeI 263 GCCGGC GCC^GGC
384 GCCGGC
NgoMIV 263 GCCGGC G^CCGGC
384 GCCGGC
NspV 965 TTCGAA TT^CGAA
1628 TTCGAA
PleI 993 GAGTC GAGTC(4/5)
1532 GAGTC
SfcI 1029 CTACAG C^T[GA][CT]AG
1575 CTACAG
SfuI 965 TTCGAA TT^CGAA
1628 TTCGAA
SmaI 238 CCCGGG CCC^GGG
544 CCCGGG
StuI 834 AGGCCT AGG^CCT
1548 AGGCCT
StyI 596 CCAAGG C^C[AT][AT]GG
1076 CCAAGG
XmaCI 238 CCCGGG C^CCGGG
544 CCCGGG
XmaI 238 CCCGGG C^CCGGG
544 CCCGGG
XmnI 229 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1546 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AvaI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I