################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AgeI AocI Aor51HI AscI Asp700I Asp718I AspI AviII BbeI BbvCI BsaBI BseAI BsiWI BspDI BspEI BssHII BstUI Bsu36I ClaI CpoI DraIII Eco47III Eco81I FseI FspI HindIII HpaI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PflFI PinAI PmeI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AflII 5113 CTTAAG C^TTAAG AseI 4606 ATTAAT AT^TAAT AsnI 4606 ATTAAT AT^TAAT AvrII 207 CCTAGG C^CTAGG BamHI 4166 GGATCC G^GATCC BfrI 5113 CTTAAG C^TTAAG BglI 6608 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 207 CCTAGG C^CTAGG BlpI 97 GCTCAGC GC^T[ACGT]AGC Bpu1102I 97 GCTCAGC GC^T[ACGT]AGC BsaWI 5014 ACCGGA [AT]^CCGG[AT] BsiEI 789 CGGCCG CG[GA][CT]^CG BsmI 1890 GAATGC GAATGC(1/-1) Bst1107I 2026 GTATAC GTA^TAC BstBI 1167 TTCGAA TT^CGAA BstZ17I 2026 GTATAC GTA^TAC CelII 97 GCTCAGC GC^T[ACGT]AGC DraI 6322 TTTAAA TTT^AAA EagI 789 CGGCCG C^GGCCG EclXI 789 CGGCCG C^GGCCG Eco52I 789 CGGCCG C^GGCCG EcoT22I 1521 ATGCAT ATGCA^T NsiI 1521 ATGCAT ATGCA^T NspV 1167 TTCGAA TT^CGAA PshAI 1465 GACATGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4606 ATTAAT AT^TAAT SalI 1672 GTCGAC G^TCGAC SexAI 2613 ACCTGGT A^CC[AT]GGT SfuI 1167 TTCGAA TT^CGAA SmaI 19 CCCGGG CCC^GGG XmaCI 19 CCCGGG C^CCGGG XmaI 19 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 3321 GACGCC G[GA]^CG[CT]C 5422 GACGCC AhdI 1854 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2233 GACACCGTGTC Alw44I 1485 GTGCAC G^TGCAC 4126 GTGCAC ApaLI 1485 GTGCAC G^TGCAC 4126 GTGCAC AspEI 1854 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2233 GACACCGTGTC BciVI 3076 GGATAC GTATCC(6/5) 3190 GTATCC BglII 5369 AGATCT A^GATCT 5756 AGATCT BsaHI 3321 GACGCC G[GA]^CG[CT]C 5422 GACGCC BsmBI 2140 GAGACG CGTCTC(1/5) 5245 GAGACG BspHI 1602 TCATGA T^CATGA 6507 TCATGA BspLU11I 488 ACATGT A^CATGT 6466 ACATGT BsrBI 196 GAGCGG CCGCTC(-3/-3) 840 GAGCGG BsrFI 2803 ACCGGC [GA]^CCGG[CT] 5521 ACCGGC BstAPI 665 GCATCACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2853 GCAGAGGCTGC Cfr10I 2803 ACCGGC [GA]^CCGG[CT] 5521 ACCGGC DrdI 2758 GACGTGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3513 GACACCATTGTC Eam1105I 1854 GACTCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2233 GACACCGTGTC EcoRI 4230 GAATTC G^AATTC 6490 GAATTC EcoRV 2376 GATATC GAT^ATC 4982 GATATC Hin1I 3321 GACGCC G[GA]^CG[CT]C 5422 GACGCC NcoI 1385 CCATGG C^CATGG 2943 CCATGG RcaI 1602 TCATGA T^CATGA 6507 TCATGA SapI 81 GAAGAGC GCTCTTC(1/4) 5670 GAAGAGC StuI 2007 AGGCCT AGG^CCT 6117 AGGCCT XbaI 679 TCTAGA T^CTAGA 6379 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvI BcgI BclI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PaeR7I PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII