Restriction map for
pF1KB0019
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# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccIII AcyI AgeI AhdI
Aor51HI ApaI AscI AspEI AviII
BalI BamHI BbeI BbvCI BcgI
BsaHI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BssHII BssSI BstEII
BstPI ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FauI FseI FspI Hin1I
HpaI KasI KspI MluI MluNI
MroI MscI NaeI NarI NcoI
NgoMIV NotI PacI PinAI PmeI
PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 613 GGTACC G^GTACC
AclI 3434 AACGTT AA^CGTT
Alw44I 1832 GTGCAC G^TGCAC
ApaLI 1832 GTGCAC G^TGCAC
Asp718I 613 GGTACC G^GTACC
AspI 5242 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 8368 CCTAGG C^CTAGG
BanII 5971 GAGCCC G[GA]GC[CT]^C
BbrPI 5360 CACGTG CAC^GTG
BciVI 7816 GTATCC GTATCC(6/5)
BglI 1540 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 8368 CCTAGG C^CTAGG
Bsp1407I 4841 TGTACA T^GTACA
BsrBI 772 GAGCGG CCGCTC(-3/-3)
BsrFI 754 ACCGGC [GA]^CCGG[CT]
BsrGI 4841 TGTACA T^GTACA
Bst1107I 689 GTATAC GTA^TAC
BstBI 6707 TTCGAA TT^CGAA
BstZ17I 689 GTATAC GTA^TAC
Cfr10I 754 ACCGGC [GA]^CCGG[CT]
DraIII 8315 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
KpnI 613 GGTACC GGTAC^C
MfeI 562 CAATTG C^AATTG
MunI 562 CAATTG C^AATTG
NheI 1002 GCTAGC G^CTAGC
NruI 991 TCGCGA TCG^CGA
NspV 6707 TTCGAA TT^CGAA
PflFI 5242 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 5360 CACGTG CAC^GTG
PmlI 5360 CACGTG CAC^GTG
PshAI 2022 GACAATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3434 AACGTT AA^CGTT
SapI 1378 GAAGAGC GCTCTTC(1/4)
SexAI 5243 ACCAGGT A^CC[AT]GGT
SfuI 6707 TTCGAA TT^CGAA
SmaI 1118 CCCGGG CCC^GGG
SphI 2114 GCATGC GCATG^C
SspBI 4841 TGTACA T^GTACA
StuI 2197 AGGCCT AGG^CCT
SwaI 5393 ATTTAAAT ATTT^AAAT
Tth111I 5242 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1118 CCCGGG C^CCGGG
XmaI 1118 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AflII 6757 CTTAAG C^TTAAG
7353 CTTAAG
AocI 7073 CCTCAGG CC^T[ACGT]AGG
7892 CCTCAGG
AseI 3361 ATTAAT AT^TAAT
5182 ATTAAT
AsnI 3361 ATTAAT AT^TAAT
5182 ATTAAT
BanI 613 GGTACC G^G[CT][GA]CC
7941 GGCACC
BclI 18 TGATCA T^GATCA
6456 TGATCA
BfrI 6757 CTTAAG C^TTAAG
7353 CTTAAG
BlpI 769 GCTGAGC GC^T[ACGT]AGC
2296 GCTCAGC
Bpu1102I 769 GCTGAGC GC^T[ACGT]AGC
2296 GCTCAGC
BsaAI 4763 CACGTA [CT]AC^GT[GA]
5360 CACGTG
BsaBI 302 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6902 GATCAGGATC
BsaWI 1865 ACCGGA [AT]^CCGG[AT]
5877 TCCGGT
BsmI 5176 GAATGC GAATGC(1/-1)
7158 GAATGC
BspHI 972 TCATGA T^CATGA
1278 TCATGA
BspLU11I 2836 ACATGT A^CATGT
7770 ACATGT
Bsu36I 7073 CCTCAGG CC^T[ACGT]AGG
7892 CCTCAGG
CelII 769 GCTGAGC GC^T[ACGT]AGC
2296 GCTCAGC
DraII 4682 AGGACCT [GA]G^G[ACGT]CC[CT]
7426 AGGTCCC
EaeI 6972 TGGCCG [CT]^GGCC[GA]
8401 CGGCCA
Eco81I 7073 CCTCAGG CC^T[ACGT]AGG
7892 CCTCAGG
EcoO109I 4682 AGGACCT [GA]G^G[ACGT]CC[CT]
7426 AGGTCCC
EcoRI 659 GAATTC G^AATTC
6497 GAATTC
EcoT22I 2036 ATGCAT ATGCA^T
5178 ATGCAT
FbaI 18 TGATCA T^GATCA
6456 TGATCA
HaeII 7571 AGCGCC [GA]GCGC^[CT]
8495 AGCGCC
HgaI 210 GCGTC GACGC(5/10)
5263 GCGTC
HincII 5614 GTTGAC GT[CT]^[GA]AC
7320 GTCAAC
HindII 5614 GTTGAC GT[CT]^[GA]AC
7320 GTCAAC
MamI 302 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6902 GATCAGGATC
NsiI 2036 ATGCAT ATGCA^T
5178 ATGCAT
PaeR7I 6674 CTCGAG C^TCGAG
7676 CTCGAG
PpuMI 4682 AGGACCT [GA]G^G[AT]CC[CT]
7426 AGGTCCC
PshBI 3361 ATTAAT AT^TAAT
5182 ATTAAT
RcaI 972 TCATGA T^CATGA
1278 TCATGA
SspI 2221 AATATT AAT^ATT
3089 AATATT
XhoI 6674 CTCGAG C^TCGAG
7676 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BbsI
BbvI BcnI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EarI EcoNI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI
XhoII XmnI