Restriction map for
pF1KB0044
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AspEI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BclI BcnI
BfrI BglI BlnI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI BspEI BspLU11I BspMI BsrBI
BssHII Bst1107I BstEII BstPI BstUI
BstZ17I Bsu36I CfoI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV FbaI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII KasI KspI MfeI MluI
MluNI MroI MscI MunI MvnI
NarI NciI NdeI NheI NotI
NruI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 1636 GGTACC G^GTACC
AflIII 1878 ACACGT A^C[GA][CT]GT
AseI 2415 ATTAAT AT^TAAT
AsnI 2415 ATTAAT AT^TAAT
Asp718I 1636 GGTACC G^GTACC
AspI 560 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1636 GGTACC G^G[CT][GA]CC
BbvCI 1609 GCTGAGG CCTCAGC(-5/-2)
BcgI 3549 GCATGCTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2985 GTATCC GTATCC(6/5)
BglII 3458 AGATCT A^GATCT
BsaAI 1879 CACGTA [CT]AC^GT[GA]
BsaBI 3453 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3389 GAGACC GGTCTC(1/5)
Bsp1407I 1591 TGTACA T^GTACA
BspDI 2000 ATCGAT AT^CGAT
BsrGI 1591 TGTACA T^GTACA
BssSI 2909 CACGAG CACGAG(-5/-1)
BstAPI 2205 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 416 TTCGAA TT^CGAA
BstXI 2841 CCATGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2000 ATCGAT AT^CGAT
DraI 2956 TTTAAA TTT^AAA
DraIII 1252 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 120 TGGCCG [CT]^GGCC[GA]
EcoNI 1528 CCTGTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2647 ATGCAT ATGCA^T
FauI 3366 GCGGG CCCGC(4/6)
FseI 121 GGCCGGCC GGCCGG^CC
HpaI 2091 GTTAAC GTT^AAC
KpnI 1636 GGTACC GGTAC^C
MamI 3453 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 122 GCCGGC GCC^GGC
NgoMIV 122 GCCGGC G^CCGGC
NsiI 2647 ATGCAT ATGCA^T
NspV 416 TTCGAA TT^CGAA
PflFI 560 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2814 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2415 ATTAAT AT^TAAT
SacI 1470 GAGCTC GAGCT^C
ScaI 3015 AGTACT AGT^ACT
SfuI 416 TTCGAA TT^CGAA
SmlI 1475 CTCAAG C^T[CT][GA]AG
SspBI 1591 TGTACA T^GTACA
StuI 2579 AGGCCT AGG^CCT
SwaI 2955 ATTTAAAT ATTT^AAAT
Tth111I 560 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1896 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AciI 2088 GCGG CCGC(-3/-1)
3366 GCGG
AlwNI 2332 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2462 CAGATACTG
AspHI 1470 GAGCTC G[AT]GC[AT]^C
1568 GTGCTC
BanII 1470 GAGCTC G[GA]GC[CT]^C
3583 GAGCCC
BlpI 3580 GCTGAGC GC^T[ACGT]AGC
3700 GCTGAGC
Bpu1102I 3580 GCTGAGC GC^T[ACGT]AGC
3700 GCTGAGC
BsiHKAI 1470 GAGCTC G[AT]GC[AT]^C
1568 GTGCTC
BsmAI 744 GAGAC GTCTC(1/5)
3389 GAGAC
BsmFI 338 GGGAC GGGAC(10/14)
2816 GTCCC
BsmI 429 GAATGC GAATGC(1/-1)
3761 GAATGC
BspHI 686 TCATGA T^CATGA
1017 TCATGA
BsrDI 289 GCAATG GCAATG(2/0)
1546 GCAATG
BsrFI 122 GCCGGC [GA]^CCGG[CT]
1639 ACCGGC
BstYI 2130 GGATCT [GA]^GATC[CT]
3458 AGATCT
CelII 3580 GCTGAGC GC^T[ACGT]AGC
3700 GCTGAGC
Cfr10I 122 GCCGGC [GA]^CCGG[CT]
1639 ACCGGC
DraII 2435 AGGGCCT [GA]G^G[ACGT]CC[CT]
2814 AGGTCCC
DrdI 621 GACGGGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
634 GACTCCCAGGTC
EcoO109I 2435 AGGGCCT [GA]G^G[ACGT]CC[CT]
2814 AGGTCCC
EcoT14I 3383 CCATGG C^C[AT][AT]GG
3656 CCATGG
HapII 123 CCGG C^CGG
1640 CCGG
HpaII 123 CCGG C^CGG
1640 CCGG
MflI 2130 GGATCT [GA]^GATC[CT]
3458 AGATCT
MspI 123 CCGG C^CGG
1640 CCGG
NcoI 3383 CCATGG C^CATGG
3656 CCATGG
PflMI 2461 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2535 CCAGAGCATGG
RcaI 686 TCATGA T^CATGA
1017 TCATGA
SapI 168 GAAGAGC GCTCTTC(1/4)
209 GAAGAGC
SphI 2649 GCATGC GCATG^C
3549 GCATGC
StyI 3383 CCATGG C^C[AT][AT]GG
3656 CCATGG
Tsp45I 253 GTCAC ^GT[GC]AC
3157 GTCAC
Van91I 2461 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2535 CCAGAGCATGG
XcmI 54 CCATTCCGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
788 CCAATGAAAGGTTGG
XhoII 2130 GGATCT [GA]^GATC[CT]
3458 AGATCT
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# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AvaII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DsaI EarI EcoRII Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XmnI