Restriction map for
pF1KB0049
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# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
AscI Asp718I AspEI AspI AvrII
BfrI BlnI BspDI BsrFI BssHII
Cfr10I ClaI CpoI DraI DrdI
Eam1105I EcoRI FseI HindIII HpaI
KpnI KspI MluI NaeI NdeI
NgoMIV NheI NotI NruI PacI
PflFI PflMI PinAI PmeI PshAI
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I Van91I XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 5044 TCCGGA T^CCGGA
AocI 2929 CCTGAGG CC^T[ACGT]AGG
ApaI 3557 GGGCCC GGGCC^C
AseI 3391 ATTAAT AT^TAAT
AsnI 3391 ATTAAT AT^TAAT
AviII 3305 TGCGCA TGC^GCA
BbeI 5437 GGCGCC GGCGC^C
BbrPI 928 CACGTG CAC^GTG
BseAI 5044 TCCGGA T^CCGGA
BsiWI 2398 CGTACG C^GTACG
BspEI 5044 TCCGGA T^CCGGA
BspHI 4556 TCATGA T^CATGA
BssSI 1324 CTCGTG CACGAG(-5/-1)
BstBI 3236 TTCGAA TT^CGAA
BstEII 3313 GGTTACC G^GT[ACGT]ACC
BstPI 3313 GGTTACC G^GT[ACGT]ACC
Bsu36I 2929 CCTGAGG CC^T[ACGT]AGG
Eco81I 2929 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3313 GGTTACC G^GT[ACGT]ACC
EcoRV 3583 GATATC GAT^ATC
FspI 3305 TGCGCA TGC^GCA
KasI 5437 GGCGCC G^GCGCC
MfeI 5285 CAATTG C^AATTG
MroI 5044 TCCGGA T^CCGGA
MunI 5285 CAATTG C^AATTG
NarI 5437 GGCGCC GG^CGCC
NspV 3236 TTCGAA TT^CGAA
PaeR7I 2541 CTCGAG C^TCGAG
PmaCI 928 CACGTG CAC^GTG
PmlI 928 CACGTG CAC^GTG
PshBI 3391 ATTAAT AT^TAAT
RcaI 4556 TCATGA T^CATGA
SapI 1219 GAAGAGC GCTCTTC(1/4)
SfoI 5437 GGCGCC GGC^GCC
SfuI 3236 TTCGAA TT^CGAA
SmlI 2541 CTCGAG C^T[CT][GA]AG
StuI 2567 AGGCCT AGG^CCT
XhoI 2541 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 37 enzymes) #
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AccI 2713 GTATAC GT^[AC][GT]AC
4170 GTATAC
AclI 665 AACGTT AA^CGTT
2428 AACGTT
Alw44I 295 GTGCAC G^TGCAC
2536 GTGCAC
Aor51HI 52 AGCGCT AGC^GCT
861 AGCGCT
ApaLI 295 GTGCAC G^TGCAC
2536 GTGCAC
BamHI 2450 GGATCC G^GATCC
4317 GGATCC
BanI 4468 GGTGCC G^G[CT][GA]CC
5437 GGCGCC
BbvCI 1743 CCTCAGC CCTCAGC(-5/-2)
2377 CCTCAGC
BcgI 474 CGAAAATTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2234 CGATGGAGGTGC
BglI 33 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2257 GCCCCCATGGC
BglII 1458 AGATCT A^GATCT
3413 AGATCT
BsaAI 928 CACGTG [CT]AC^GT[GA]
4969 TACGTG
BsaBI 1008 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3414 GATCTCAATC
BsaI 1396 GAGACC GGTCTC(1/5)
4711 GGTCTC
BsiEI 188 CGGCCG CG[GA][CT]^CG
2611 CGGCCG
BsmBI 2576 CGTCTC CGTCTC(1/5)
145 GAGACG
BsmI 760 GAATGC GAATGC(1/-1)
1668 GAATGC
Bsp1407I 2588 TGTACA T^GTACA
3053 TGTACA
BsrBI 917 CCGCTC CCGCTC(-3/-3)
4342 GAGCGG
BsrGI 2588 TGTACA T^GTACA
3053 TGTACA
Bst1107I 2713 GTATAC GTA^TAC
4170 GTATAC
BstZ17I 2713 GTATAC GTA^TAC
4170 GTATAC
DraIII 5383 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
5401 CACCCAGTG
EagI 188 CGGCCG C^GGCCG
2611 CGGCCG
EclXI 188 CGGCCG C^GGCCG
2611 CGGCCG
Eco47III 52 AGCGCT AGC^GCT
861 AGCGCT
Eco52I 188 CGGCCG C^GGCCG
2611 CGGCCG
EcoNI 434 CCTGCTATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4012 CCTACCAAAGG
EcoT22I 1865 ATGCAT ATGCA^T
3600 ATGCAT
HincII 1298 GTCAAC GT[CT]^[GA]AC
3457 GTTGAC
HindII 1298 GTCAAC GT[CT]^[GA]AC
3457 GTTGAC
MamI 1008 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3414 GATCTCAATC
NsiI 1865 ATGCAT ATGCA^T
3600 ATGCAT
Psp1406I 665 AACGTT AA^CGTT
2428 AACGTT
SspBI 2588 TGTACA T^GTACA
3053 TGTACA
SspI 1885 AATATT AAT^ATT
4993 AATATT
XbaI 2200 TCTAGA T^CTAGA
4977 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanII BbsI BbvI BciVI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI