Restriction map for
pF1KB0087
##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AcyI AflII AgeI
AscI AseI AsnI Asp718I AspI
AvrII BbeI BbrPI BbvCI BcgI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaHI BsiWI BsmI Bsp1407I
BsrGI BssHII Bst1107I BstBI BstZ17I
CelII CpoI DraI EcoRV EcoT22I
FauI FseI Hin1I HpaI KasI
KpnI KspI MfeI MluI MunI
NarI NciI NdeI NotI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 541 GTCTAC GT^[AC][GT]AC
AccIII 1169 TCCGGA T^CCGGA
AclI 1009 AACGTT AA^CGTT
AflIII 2033 ACATGT A^C[GA][CT]GT
AhdI 1070 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2362 GTGCAC G^TGCAC
ApaLI 2362 GTGCAC G^TGCAC
Asp700I 2398 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1070 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2837 CTCGAG C^[CT]CG[GA]G
AviII 2696 TGCGCA TGC^GCA
BamHI 3413 GGATCC G^GATCC
BciVI 2508 GTATCC GTATCC(6/5)
BclI 1359 TGATCA T^GATCA
BsaBI 2053 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1169 TCCGGA T^CCGGA
BsmBI 400 CGTCTC CGTCTC(1/5)
BsoBI 2837 CTCGAG C^[CT]CG[GA]G
BspDI 2110 ATCGAT AT^CGAT
BspEI 1169 TCCGGA T^CCGGA
BspHI 2331 TCATGA T^CATGA
BspLU11I 2033 ACATGT A^CATGT
BstAPI 2699 GCACTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 2110 ATCGAT AT^CGAT
DraIII 2700 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3120 GACCTCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 74 CGGCCG C^GGCCG
Eam1105I 1070 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 74 CGGCCG C^GGCCG
Eco52I 74 CGGCCG C^GGCCG
EcoRI 601 GAATTC G^AATTC
FbaI 1359 TGATCA T^GATCA
FspI 2696 TGCGCA TGC^GCA
HgaI 399 GCGTC GACGC(5/10)
HindIII 644 AAGCTT A^AGCTT
MamI 2053 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1169 TCCGGA T^CCGGA
NaeI 833 GCCGGC GCC^GGC
NgoMIV 833 GCCGGC G^CCGGC
NheI 2266 GCTAGC G^CTAGC
NruI 438 TCGCGA TCG^CGA
PaeR7I 2837 CTCGAG C^TCGAG
PshAI 698 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1009 AACGTT AA^CGTT
PvuII 1120 CAGCTG CAG^CTG
RcaI 2331 TCATGA T^CATGA
SacI 1573 GAGCTC GAGCT^C
SphI 1194 GCATGC GCATG^C
StuI 458 AGGCCT AGG^CCT
XhoI 2837 CTCGAG C^TCGAG
XmnI 2398 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 305 CGCG CG^CG
439 CGCG
AcsI 601 GAATTC [GA]^AATT[CT]
2623 GAATTT
ApaI 612 GGGCCC GGGCC^C
1541 GGGCCC
ApoI 601 GAATTC [GA]^AATT[CT]
2623 GAATTT
BfaI 433 CTAG C^TAG
2267 CTAG
BglII 119 AGATCT A^GATCT
2168 AGATCT
BsaAI 13 TACGTG [CT]AC^GT[GA]
565 CACGTA
BsaWI 58 ACCGGA [AT]^CCGG[AT]
1169 TCCGGA
BsgI 1729 CTGCAC GTGCAG(16/14)
2128 CTGCAC
BsiEI 74 CGGCCG CG[GA][CT]^CG
1872 CGACCG
BspMI 224 ACCTGC ACCTGC(4/8)
812 ACCTGC
BsrBI 2962 GAGCGG CCGCTC(-3/-3)
3235 GAGCGG
BsrFI 352 ACCGGC [GA]^CCGG[CT]
833 GCCGGC
BstEII 129 GGTCACC G^GT[ACGT]ACC
3117 GGTGACC
BstPI 129 GGTCACC G^GT[ACGT]ACC
3117 GGTGACC
BstUI 305 CGCG CG^CG
439 CGCG
BstXI 1448 CCAAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2976 CCAGGAGGTTGG
Cfr10I 352 ACCGGC [GA]^CCGG[CT]
833 GCCGGC
EcoO65I 129 GGTCACC G^GT[ACGT]ACC
3117 GGTGACC
MaeI 433 CTAG C^TAG
2267 CTAG
MspA1I 1120 CAGCTG C[AC]G^C[GT]G
3351 CAGCGG
MvnI 305 CGCG CG^CG
439 CGCG
PflMI 1518 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1989 CCACAAATTGG
SapI 837 GCTCTTC GCTCTTC(1/4)
3084 GCTCTTC
Van91I 1518 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1989 CCACAAATTGG
XcmI 324 CCAGGACCGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2226 CCAAGACACGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI Aor51HI AspHI AvaII BalI
BanI BanII BbsI BbvI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BssSI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII