Restriction map for
pF1KB0150
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# NO CUT ENZYMES ( 81 enzymes) #
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AatII Acc65I AccII AflII AflIII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspI AvrII
BamHI BbrPI BbsI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsiWI BspDI BspLU11I BsrBI
BssHII Bst1107I BstBI BstUI BstZ17I
CelII ClaI CpoI DraI DraIII
Eco47III EcoRI EcoRV FauI FseI
KpnI KspI MamI MluI MvnI
NaeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SfiI SfuI
SgrAI SmaI SnaBI SpeI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 2028 GTCTAC GT^[AC][GT]AC
AccIII 1149 TCCGGA T^CCGGA
AclI 403 AACGTT AA^CGTT
AhdI 3130 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3130 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 4285 CCCGAG C^[CT]CG[GA]G
AviII 924 TGCGCA TGC^GCA
BcgI 330 CGACAAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1947 TGATCA T^GATCA
BsaI 1635 GGTCTC GGTCTC(1/5)
BsaWI 1149 TCCGGA [AT]^CCGG[AT]
BseAI 1149 TCCGGA T^CCGGA
BsiEI 1856 CGGCCG CG[GA][CT]^CG
BsmBI 842 CGTCTC CGTCTC(1/5)
BsoBI 4285 CCCGAG C^[CT]CG[GA]G
Bsp1407I 4148 TGTACA T^GTACA
BspEI 1149 TCCGGA T^CCGGA
BsrGI 4148 TGTACA T^GTACA
BssSI 2472 CACGAG CACGAG(-5/-1)
BstAPI 265 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2386 GGTGACC G^GT[ACGT]ACC
BstPI 2386 GGTGACC G^GT[ACGT]ACC
BstXI 4335 CCAGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 3000 GACGCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1856 CGGCCG C^GGCCG
Eam1105I 3130 GACCAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1856 CGGCCG C^GGCCG
Eco52I 1856 CGGCCG C^GGCCG
EcoO65I 2386 GGTGACC G^GT[ACGT]ACC
FbaI 1947 TGATCA T^GATCA
FspI 924 TGCGCA TGC^GCA
HpaI 2115 GTTAAC GTT^AAC
MfeI 594 CAATTG C^AATTG
MroI 1149 TCCGGA T^CCGGA
MunI 594 CAATTG C^AATTG
NdeI 1111 CATATG CA^TATG
PflMI 4117 CCACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 403 AACGTT AA^CGTT
SacI 3230 GAGCTC GAGCT^C
SbfI 1525 CCTGCAGG CCTGCA^GG
ScaI 975 AGTACT AGT^ACT
SexAI 893 ACCTGGT A^CC[AT]GGT
Sse8387I 1525 CCTGCAGG CCTGCA^GG
SspBI 4148 TGTACA T^GTACA
SspI 4385 AATATT AAT^ATT
StuI 3661 AGGCCT AGG^CCT
Van91I 4117 CCACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AseI 2340 ATTAAT AT^TAAT
3688 ATTAAT
AsnI 2340 ATTAAT AT^TAAT
3688 ATTAAT
AspHI 2601 GAGCAC G[AT]GC[AT]^C
3230 GAGCTC
BalI 2108 TGGCCA TGG^CCA
2988 TGGCCA
BbeI 2482 GGCGCC GGCGC^C
3387 GGCGCC
BbvCI 1617 CCTCAGC CCTCAGC(-5/-2)
2743 CCTCAGC
BcnI 1854 CCCGG CC^[GC]GG
3520 CCGGG
BsiHKAI 2601 GAGCAC G[AT]GC[AT]^C
3230 GAGCTC
BsrFI 2199 GCCGGT [GA]^CCGG[CT]
2918 ACCGGC
Cfr10I 2199 GCCGGT [GA]^CCGG[CT]
2918 ACCGGC
EcoT22I 3410 ATGCAT ATGCA^T
3754 ATGCAT
HaeII 2482 GGCGCC [GA]GCGC^[CT]
3387 GGCGCC
HgaI 3000 GACGC GACGC(5/10)
3136 GCGTC
HincII 2115 GTTAAC GT[CT]^[GA]AC
2500 GTTGAC
HindII 2115 GTTAAC GT[CT]^[GA]AC
2500 GTTGAC
HindIII 1533 AAGCTT A^AGCTT
1734 AAGCTT
KasI 2482 GGCGCC G^GCGCC
3387 GGCGCC
MluNI 2108 TGGCCA TGG^CCA
2988 TGGCCA
MscI 2108 TGGCCA TGG^CCA
2988 TGGCCA
NarI 2482 GGCGCC GG^CGCC
3387 GGCGCC
NciI 1854 CCCGG CC^[GC]GG
3520 CCGGG
NsiI 3410 ATGCAT ATGCA^T
3754 ATGCAT
PshBI 2340 ATTAAT AT^TAAT
3688 ATTAAT
SapI 454 GAAGAGC GCTCTTC(1/4)
1053 GAAGAGC
SfoI 2482 GGCGCC GGC^GCC
3387 GGCGCC
SphI 1304 GCATGC GCATG^C
1355 GCATGC
XcmI 2848 CCATCTGGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3365 CCAACATCATGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AvaII
BanI BanII BbvI BciVI BfaI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII