Restriction map for
pF1KB0179
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BfrI BglI BlnI
BsaAI BseAI BsiEI BsiWI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
Bst1107I BstAPI BstBI BstZ17I Cfr10I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoT22I
FseI FspI HpaI KasI KpnI
MluI MroI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XcmI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 2628 GACGCC G[GA]^CG[CT]C
Alw44I 883 GTGCAC G^TGCAC
AocI 1279 CCTAAGG CC^T[ACGT]AGG
ApaLI 883 GTGCAC G^TGCAC
BbvCI 2147 CCTCAGC CCTCAGC(-5/-2)
BciVI 3025 GTATCC GTATCC(6/5)
BglII 1032 AGATCT A^GATCT
BlpI 3564 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3564 GCTAAGC GC^T[ACGT]AGC
BsaHI 2628 GACGCC G[GA]^CG[CT]C
BsmBI 259 CGTCTC CGTCTC(1/5)
BsmI 1271 GCATTC GAATGC(1/-1)
BspMI 3390 GCAGGT ACCTGC(4/8)
BsrDI 1426 GCAATG GCAATG(2/0)
BssSI 2620 CTCGTG CACGAG(-5/-1)
BstEII 3367 GGTTACC G^GT[ACGT]ACC
BstPI 3367 GGTTACC G^GT[ACGT]ACC
Bsu36I 1279 CCTAAGG CC^T[ACGT]AGG
CelII 3564 GCTAAGC GC^T[ACGT]AGC
DraIII 2315 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1659 GACCCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1279 CCTAAGG CC^T[ACGT]AGG
EcoNI 1803 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3367 GGTTACC G^GT[ACGT]ACC
HaeII 1166 AGCGCC [GA]GCGC^[CT]
Hin1I 2628 GACGCC G[GA]^CG[CT]C
HincII 1528 GTTGAC GT[CT]^[GA]AC
HindII 1528 GTTGAC GT[CT]^[GA]AC
HindIII 449 AAGCTT A^AGCTT
KspI 53 CCGCGG CCGC^GG
MfeI 1717 CAATTG C^AATTG
MunI 1717 CAATTG C^AATTG
NheI 3135 GCTAGC G^CTAGC
SacI 895 GAGCTC GAGCT^C
SacII 53 CCGCGG CCGC^GG
SexAI 2316 ACCAGGT A^CC[AT]GGT
SmaI 635 CCCGGG CCC^GGG
SphI 1620 GCATGC GCATG^C
StuI 876 AGGCCT AGG^CCT
XbaI 658 TCTAGA T^CTAGA
XmaCI 635 CCCGGG C^CCGGG
XmaI 635 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccII 54 CGCG CG^CG
2037 CGCG
ApaI 2894 GGGCCC GGGCC^C
3221 GGGCCC
Asp700I 1240 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3045 GAACGTCTTC
BbsI 2332 GTCTTC GAAGAC(2/6)
3049 GTCTTC
BcgI 1864 GCAGCTATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2524 GCAGAGACATCG
BclI 158 TGATCA T^GATCA
2861 TGATCA
BpuAI 2332 GTCTTC GAAGAC(2/6)
3049 GTCTTC
BsaBI 1009 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1665 GATGTCAATC
BsrBI 612 CCGCTC CCGCTC(-3/-3)
727 GAGCGG
BstUI 54 CGCG CG^CG
2037 CGCG
BstXI 1189 CCAGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1853 CCAGAAGAGTGG
EcoRV 662 GATATC GAT^ATC
2323 GATATC
FbaI 158 TGATCA T^GATCA
2861 TGATCA
MamI 1009 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1665 GATGTCAATC
MvnI 54 CGCG CG^CG
2037 CGCG
PaeR7I 73 CTCGAG C^TCGAG
3641 CTCGAG
PstI 3000 CTGCAG CTGCA^G
3321 CTGCAG
PvuII 847 CAGCTG CAG^CTG
2878 CAGCTG
XhoI 73 CTCGAG C^TCGAG
3641 CTCGAG
XmnI 1240 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3045 GAACGTCTTC
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BalI BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII