Restriction map for
pF1KB0356
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AflII AgeI AlwNI Aor51HI ApaI
AscI Asp700I Asp718I AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BclI BcnI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BstEII
BstPI BstUI CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FauI FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MluI MroI MspA1I
MspI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 618 GTATAC GT^[AC][GT]AC
AciI 2591 GCGG CCGC(-3/-1)
AclI 433 AACGTT AA^CGTT
AhdI 2359 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2289 CCTCAGG CC^T[ACGT]AGG
AseI 123 ATTAAT AT^TAAT
AsnI 123 ATTAAT AT^TAAT
AspEI 2359 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1502 CCCGAG C^[CT]CG[GA]G
BalI 1336 TGGCCA TGG^CCA
BanII 2900 GAGCCC G[GA]GC[CT]^C
BglII 1058 AGATCT A^GATCT
BsaAI 1027 TACGTG [CT]AC^GT[GA]
BsaBI 3133 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 1502 CCCGAG C^[CT]CG[GA]G
BspHI 3116 TCATGA T^CATGA
BssKI 1142 CCAGG ^CC[ACGT]GG
Bst1107I 618 GTATAC GTA^TAC
BstAPI 2424 GCAAGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 1142 CCAGG CC^[AT]GG
BstZ17I 618 GTATAC GTA^TAC
Bsu36I 2289 CCTCAGG CC^T[ACGT]AGG
DsaI 1083 CCATGG C^C[GA][CT]GG
EaeI 1336 TGGCCA [CT]^GGCC[GA]
Eam1105I 2359 GACATCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2289 CCTCAGG CC^T[ACGT]AGG
EcoNI 108 CCTCCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1722 GAATTC G^AATTC
EcoRII 1142 CCAGG ^CC[AT]GG
EcoT14I 1083 CCATGG C^C[AT][AT]GG
EcoT22I 3194 ATGCAT ATGCA^T
HgaI 926 GACGC GACGC(5/10)
MamI 3133 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1488 CAATTG C^AATTG
MluNI 1336 TGGCCA TGG^CCA
MscI 1336 TGGCCA TGG^CCA
MunI 1488 CAATTG C^AATTG
MvaI 1142 CCAGG CC^[AT]GG
NcoI 1083 CCATGG C^CATGG
NsiI 3194 ATGCAT ATGCA^T
PshBI 123 ATTAAT AT^TAAT
Psp1406I 433 AACGTT AA^CGTT
PstI 2608 CTGCAG CTGCA^G
RcaI 3116 TCATGA T^CATGA
SapI 1477 GAAGAGC GCTCTTC(1/4)
ScrFI 1142 CCAGG CC^[ACGT]GG
SmlI 221 CTCAAG C^T[CT][GA]AG
StyI 1083 CCATGG C^C[AT][AT]GG
XbaI 1726 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 2692 ACACGT A^C[GA][CT]GT
2850 ACGTGT
Alw44I 995 GTGCAC G^TGCAC
2603 GTGCAC
ApaLI 995 GTGCAC G^TGCAC
2603 GTGCAC
AspHI 995 GTGCAC G[AT]GC[AT]^C
2603 GTGCAC
AvaII 462 GGACC G^G[AT]CC
1911 GGTCC
BbvI 2674 GCAGC GCAGC(8/12)
1195 GCTGC
BciVI 2707 GGATAC GTATCC(6/5)
1498 GTATCC
BsaJI 1083 CCATGG C^C[ACGT][ACGT]GG
1141 CCCAGG
BseRI 2258 CTCCTC GAGGAG(10/8)
1088 GAGGAG
BsiHKAI 995 GTGCAC G[AT]GC[AT]^C
2603 GTGCAC
BsmFI 461 GGGAC GGGAC(10/14)
2535 GGGAC
BssSI 1607 CACGAG CACGAG(-5/-1)
1190 CTCGTG
BstXI 1261 CCATTGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2043 CCAGAGGTTTGG
Cfr13I 462 GGACC G^G[ACGT]CC
1911 GGTCC
DraII 461 GGGACCT [GA]G^G[ACGT]CC[CT]
1910 AGGTCCT
EcoO109I 461 GGGACCT [GA]G^G[ACGT]CC[CT]
1910 AGGTCCT
NlaIV 461 GGGACC GG[ACGT]^[ACGT]CC
2899 GGAGCC
PpuMI 461 GGGACCT [GA]G^G[AT]CC[CT]
1910 AGGTCCT
Sau96I 462 GGACC G^G[ACGT]CC
1911 GGTCC
SfcI 2242 CTACAG C^T[GA][CT]AG
2608 CTGCAG
TseI 1195 GCTGC G^C[AT]GC
2674 GCAGC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BfaI BmyI BsiYI BslI BsmAI
BsmI Bsp1286I BsrDI BsrI BstBI
BstF5I BstYI Cac8I DdeI DpnII
DraI EarI Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MwoI NdeII
NlaIII NspI NspV PleI RsaI
Sau3AI SfaNI SfuI SphI SspI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I XhoII