Restriction map for
pF1KB0869
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# NO CUT ENZYMES ( 82 enzymes) #
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AccIII AclI AflII AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII BbeI
BfrI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FseI FspI HindIII KasI KspI
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PmeI
PshBI Psp1406I PvuI RsrII SacII
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 2370 GACGTC GACGT^C
AccI 1219 GTCGAC GT^[AC][GT]AC
AgeI 377 ACCGGT A^CCGGT
AvaI 752 CCCGGG C^[CT]CG[GA]G
AvrII 591 CCTAGG C^CTAGG
BalI 1265 TGGCCA TGG^CCA
BamHI 1681 GGATCC G^GATCC
BbvCI 947 CCTCAGC CCTCAGC(-5/-2)
BciVI 1041 GTATCC GTATCC(6/5)
BclI 456 TGATCA T^GATCA
BglII 3198 AGATCT A^GATCT
BlnI 591 CCTAGG C^CTAGG
BlpI 2925 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2925 GCTGAGC GC^T[ACGT]AGC
BsmBI 3024 CGTCTC CGTCTC(1/5)
BsoBI 752 CCCGGG C^[CT]CG[GA]G
BspHI 812 TCATGA T^CATGA
BssSI 1806 CTCGTG CACGAG(-5/-1)
BstXI 2686 CCAGAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2925 GCTGAGC GC^T[ACGT]AGC
EagI 2812 CGGCCG C^GGCCG
EclXI 2812 CGGCCG C^GGCCG
Eco52I 2812 CGGCCG C^GGCCG
FbaI 456 TGATCA T^GATCA
HpaI 180 GTTAAC GTT^AAC
MluNI 1265 TGGCCA TGG^CCA
MscI 1265 TGGCCA TGG^CCA
NaeI 2810 GCCGGC GCC^GGC
NgoMIV 2810 GCCGGC G^CCGGC
PinAI 377 ACCGGT A^CCGGT
PshAI 2139 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 812 TCATGA T^CATGA
SalI 1219 GTCGAC G^TCGAC
SapI 1347 GCTCTTC GCTCTTC(1/4)
ScaI 877 AGTACT AGT^ACT
SmaI 752 CCCGGG CCC^GGG
XmaCI 752 CCCGGG C^CCGGG
XmaI 752 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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Acc65I 1299 GGTACC G^GTACC
2984 GGTACC
AcsI 678 AAATTT [GA]^AATT[CT]
2220 GAATTT
AflIII 1958 ACATGT A^C[GA][CT]GT
2900 ACATGT
ApoI 678 AAATTT [GA]^AATT[CT]
2220 GAATTT
Asp718I 1299 GGTACC G^GTACC
2984 GGTACC
BbrPI 1210 CACGTG CAC^GTG
1432 CACGTG
BcgI 2188 CGATACACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1757 GCATGGAGATCG
BglI 2203 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3097 GCCATCAAGGC
BpmI 741 CTCCAG CTGGAG(16/14)
1546 CTGGAG
BsaI 1637 GGTCTC GGTCTC(1/5)
1811 GGTCTC
BsaWI 377 ACCGGT [AT]^CCGG[AT]
2417 ACCGGA
BsiEI 375 CGACCG CG[GA][CT]^CG
2812 CGGCCG
BspLU11I 1958 ACATGT A^CATGT
2900 ACATGT
BspMI 1483 ACCTGC ACCTGC(4/8)
3030 ACCTGC
BsrFI 377 ACCGGT [GA]^CCGG[CT]
2810 GCCGGC
Cfr10I 377 ACCGGT [GA]^CCGG[CT]
2810 GCCGGC
DrdI 1803 GACCTCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2370 GACGTCGTGGTC
EcoT22I 401 ATGCAT ATGCA^T
1144 ATGCAT
KpnI 1299 GGTACC GGTAC^C
2984 GGTACC
NcoI 551 CCATGG C^CATGG
2774 CCATGG
NsiI 401 ATGCAT ATGCA^T
1144 ATGCAT
PmaCI 1210 CACGTG CAC^GTG
1432 CACGTG
PmlI 1210 CACGTG CAC^GTG
1432 CACGTG
SacI 532 GAGCTC GAGCT^C
1345 GAGCTC
StuI 305 AGGCCT AGG^CCT
986 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrBI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII