Restriction map for
pF1KB1986
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BclI BlnI BsaBI
BseAI BsiWI Bsp1407I BspEI BspLU11I
BspMI BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I Cfr10I
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoT22I FbaI FseI FspI
HaeII KasI KpnI KspI MamI
MluI MroI NaeI NarI NcoI
NgoMIV NheI NotI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1243 GTCTAC GT^[AC][GT]AC
AclI 2218 AACGTT AA^CGTT
AcyI 21 GGCGTC G[GA]^CG[CT]C
AfaI 1642 GTAC GT^AC
AflII 2320 CTTAAG C^TTAAG
AflIII 2718 ACACGT A^C[GA][CT]GT
AlwNI 2164 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 576 CCTCAGG CC^T[ACGT]AGG
BalI 624 TGGCCA TGG^CCA
BfrI 2320 CTTAAG C^TTAAG
BglI 2035 GCCAAATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 268 GCTCAGC GC^T[ACGT]AGC
BpmI 2227 CTGGAG CTGGAG(16/14)
Bpu1102I 268 GCTCAGC GC^T[ACGT]AGC
BsaAI 2719 CACGTA [CT]AC^GT[GA]
BsaHI 21 GGCGTC G[GA]^CG[CT]C
BsaI 3230 GAGACC GGTCTC(1/5)
BsaWI 600 TCCGGT [AT]^CCGG[AT]
BsgI 3218 GTGCAG GTGCAG(16/14)
BsiEI 532 CGATCG CG[GA][CT]^CG
BsmFI 1824 GGGAC GGGAC(10/14)
BspDI 1444 ATCGAT AT^CGAT
BspHI 522 TCATGA T^CATGA
BsrBI 29 GAGCGG CCGCTC(-3/-3)
BsrDI 1688 CATTGC GCAATG(2/0)
BstAPI 3085 GCAAAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2174 CCATCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 576 CCTCAGG CC^T[ACGT]AGG
CelII 268 GCTCAGC GC^T[ACGT]AGC
ClaI 1444 ATCGAT AT^CGAT
EaeI 624 TGGCCA [CT]^GGCC[GA]
Eco81I 576 CCTCAGG CC^T[ACGT]AGG
EcoNI 1861 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2689 GATATC GAT^ATC
HgaI 22 GCGTC GACGC(5/10)
Hin1I 21 GGCGTC G[GA]^CG[CT]C
HincII 613 GTTAAC GT[CT]^[GA]AC
HindII 613 GTTAAC GT[CT]^[GA]AC
HpaI 613 GTTAAC GTT^AAC
MluNI 624 TGGCCA TGG^CCA
MscI 624 TGGCCA TGG^CCA
NdeI 2201 CATATG CA^TATG
NruI 245 TCGCGA TCG^CGA
Psp1406I 2218 AACGTT AA^CGTT
PvuI 532 CGATCG CGAT^CG
PvuII 606 CAGCTG CAG^CTG
RcaI 522 TCATGA T^CATGA
RsaI 1642 GTAC GT^AC
ScaI 1641 AGTACT AGT^ACT
SmaI 10 CCCGGG CCC^GGG
XmaCI 10 CCCGGG C^CCGGG
XmaI 10 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AspHI 1742 GAGCTC G[AT]GC[AT]^C
2373 GAGCTC
AvaI 10 CCCGGG C^[CT]CG[GA]G
2882 CCCGAG
AvaII 1669 GGTCC G^G[AT]CC
1825 GGACC
BamHI 1410 GGATCC G^GATCC
2148 GGATCC
BanII 1742 GAGCTC G[GA]GC[CT]^C
2373 GAGCTC
BciVI 1787 GGATAC GTATCC(6/5)
2408 GTATCC
BglII 1110 AGATCT A^GATCT
2025 AGATCT
BmyI 1742 GAGCTC G[AGT]GC[ACT]^C
2373 GAGCTC
BsiHKAI 1742 GAGCTC G[AT]GC[AT]^C
2373 GAGCTC
BsmBI 1201 CGTCTC CGTCTC(1/5)
2398 CGTCTC
BsmI 493 GAATGC GAATGC(1/-1)
3223 GCATTC
BsoBI 10 CCCGGG C^[CT]CG[GA]G
2882 CCCGAG
Bsp1286I 1742 GAGCTC G[AGT]GC[ACT]^C
2373 GAGCTC
BstBI 1055 TTCGAA TT^CGAA
1625 TTCGAA
CfoI 80 GCGC GCG^C
1723 GCGC
DraII 1668 AGGTCCT [GA]G^G[ACGT]CC[CT]
1824 GGGACCT
EcoO109I 1668 AGGTCCT [GA]G^G[ACGT]CC[CT]
1824 GGGACCT
EcoT14I 1845 CCTTGG C^C[AT][AT]GG
3250 CCAAGG
HhaI 80 GCGC GCG^C
1723 GCGC
HinP1I 80 GCGC G^CGC
1723 GCGC
HindIII 664 AAGCTT A^AGCTT
2505 AAGCTT
MslI 1396 CACTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3271 CATCCCTGTG
MspA1I 606 CAGCTG C[AC]G^C[GT]G
2627 CAGCGG
NspV 1055 TTCGAA TT^CGAA
1625 TTCGAA
PflMI 1840 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2574 CCAGTCTTTGG
PpuMI 1668 AGGTCCT [GA]G^G[AT]CC[CT]
1824 GGGACCT
SacI 1742 GAGCTC GAGCT^C
2373 GAGCTC
SfaNI 2459 GCATC GCATC(5/9)
2770 GCATC
SfuI 1055 TTCGAA TT^CGAA
1625 TTCGAA
SphI 1007 GCATGC GCATG^C
2195 GCATGC
SspI 691 AATATT AAT^ATT
1352 AATATT
StyI 1845 CCTTGG C^C[AT][AT]GG
3250 CCAAGG
Van91I 1840 CCACCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2574 CCAGTCTTTGG
XbaI 891 TCTAGA T^CTAGA
3198 TCTAGA
XcmI 401 CCAAACGTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
627 CCATGAAGGTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI Asp700I BbsI BbvI BcnI
BfaI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MspI
MunI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI Sau3AI Sau96I ScrFI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI