Restriction map for
pF1KB2323
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AspEI AviII
AvrII BbvCI BcgI BciVI BclI
BfrI BglI BlnI BsaI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
BstUI ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FbaI FseI FspI
HgaI KspI MfeI MluI MroI
MunI MvnI NaeI NgoMIV NotI
NruI NsiI NspI PacI PaeR7I
PinAI PmeI PshAI Psp1406I RsrII
SacII SalI SapI SbfI ScaI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 277 GACGTC GACGT^C
AflIII 2439 ACGTGT A^C[GA][CT]GT
AocI 1212 CCTGAGG CC^T[ACGT]AGG
AseI 2770 ATTAAT AT^TAAT
AsnI 2770 ATTAAT AT^TAAT
Asp700I 757 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2671 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1117 GGCGCC GGCGC^C
BbrPI 1204 CACGTG CAC^GTG
BsaBI 786 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2313 CGATCG CG[GA][CT]^CG
BsmBI 275 GAGACG CGTCTC(1/5)
BspHI 1145 TCATGA T^CATGA
BsrBI 643 GAGCGG CCGCTC(-3/-3)
BsrDI 128 GCAATG GCAATG(2/0)
BsrFI 1696 GCCGGT [GA]^CCGG[CT]
Bst1107I 636 GTATAC GTA^TAC
BstBI 1778 TTCGAA TT^CGAA
BstEII 106 GGTTACC G^GT[ACGT]ACC
BstPI 106 GGTTACC G^GT[ACGT]ACC
BstXI 2758 CCAAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 636 GTATAC GTA^TAC
Bsu36I 1212 CCTGAGG CC^T[ACGT]AGG
CfoI 1118 GCGC GCG^C
Cfr10I 1696 GCCGGT [GA]^CCGG[CT]
DrdI 1839 GACGAAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1212 CCTGAGG CC^T[ACGT]AGG
EcoNI 555 CCTAACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 106 GGTTACC G^GT[ACGT]ACC
EcoRI 2034 GAATTC G^AATTC
FauI 261 GCGGG CCCGC(4/6)
HaeII 1117 GGCGCC [GA]GCGC^[CT]
HhaI 1118 GCGC GCG^C
HinP1I 1118 GCGC G^CGC
HpaI 1659 GTTAAC GTT^AAC
KasI 1117 GGCGCC G^GCGCC
MamI 786 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1117 GGCGCC GG^CGCC
NheI 1750 GCTAGC G^CTAGC
NspV 1778 TTCGAA TT^CGAA
PflFI 2671 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2129 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1204 CACGTG CAC^GTG
PmlI 1204 CACGTG CAC^GTG
PshBI 2770 ATTAAT AT^TAAT
PstI 2499 CTGCAG CTGCA^G
PvuI 2313 CGATCG CGAT^CG
RcaI 1145 TCATGA T^CATGA
SexAI 547 ACCTGGT A^CC[AT]GGT
SfoI 1117 GGCGCC GGC^GCC
SfuI 1778 TTCGAA TT^CGAA
TaqI 1779 TCGA T^CGA
Tth111I 2671 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1779 TCGA T^CGA
Van91I 2129 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 757 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 551 GGTACC G^GTACC
1428 GGTACC
AcyI 277 GACGTC G[GA]^CG[CT]C
1117 GGCGCC
Alw44I 1207 GTGCAC G^TGCAC
2587 GTGCAC
AlwNI 1592 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
2841 CAGGATCTG
ApaI 628 GGGCCC GGGCC^C
1101 GGGCCC
ApaLI 1207 GTGCAC G^TGCAC
2587 GTGCAC
Asp718I 551 GGTACC G^GTACC
1428 GGTACC
AvaI 1369 CCCGAG C^[CT]CG[GA]G
1965 CCCGAG
BamHI 768 GGATCC G^GATCC
827 GGATCC
BcnI 1823 CCCGG CC^[GC]GG
2102 CCCGG
BfaI 1751 CTAG C^TAG
1876 CTAG
BglII 1088 AGATCT A^GATCT
1566 AGATCT
BlpI 48 GCTCAGC GC^T[ACGT]AGC
2522 GCTCAGC
Bpu1102I 48 GCTCAGC GC^T[ACGT]AGC
2522 GCTCAGC
BsaHI 277 GACGTC G[GA]^CG[CT]C
1117 GGCGCC
BsoBI 1369 CCCGAG C^[CT]CG[GA]G
1965 CCCGAG
BstAPI 119 GCATTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
258 GCAGCGGGTGC
CelII 48 GCTCAGC GC^T[ACGT]AGC
2522 GCTCAGC
EarI 1897 GAAGAG CTCTTC(1/4)
2623 GAAGAG
Hin1I 277 GACGTC G[GA]^CG[CT]C
1117 GGCGCC
HindIII 2377 AAGCTT A^AGCTT
2683 AAGCTT
KpnI 551 GGTACC GGTAC^C
1428 GGTACC
Ksp632I 1897 GAAGAG CTCTTC(1/4)
2623 GAAGAG
MaeI 1751 CTAG C^TAG
1876 CTAG
MseI 1660 TTAA T^TAA
2771 TTAA
MslI 982 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1523 CATATGAGTG
NciI 1823 CCCGG CC^[GC]GG
2102 CCCGG
NcoI 1440 CCATGG C^CATGG
2748 CCATGG
NdeI 14 CATATG CA^TATG
1523 CATATG
PpuMI 147 GGGACCT [GA]G^G[AT]CC[CT]
681 GGGACCC
SacI 1045 GAGCTC GAGCT^C
2056 GAGCTC
TfiI 762 GATTC G^A[AT]TC
1781 GAATC
Tru9I 1660 TTAA T^TAA
2771 TTAA
XcmI 772 CCAGATGAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1475 CCACCTTCCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TseI Tsp45I
Tsp509I TspRI XhoII