Restriction map for
pF1KB3017
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI BamHI BbrPI
BclI BfrI BglI BglII BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI MamI
MfeI MroI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 1063 GTCTAC GT^[AC][GT]AC
AflIII 507 ACGCGT A^C[GA][CT]GT
AlwNI 1069 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1362 CCTGAGG CC^T[ACGT]AGG
Aor51HI 662 AGCGCT AGC^GCT
AviII 566 TGCGCA TGC^GCA
AvrII 1518 CCTAGG C^CTAGG
BbsI 1167 GTCTTC GAAGAC(2/6)
BcgI 1273 CGAGGACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 445 GGATAC GTATCC(6/5)
BfaI 1519 CTAG C^TAG
BlnI 1518 CCTAGG C^CTAGG
BlpI 330 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 330 GCTCAGC GC^T[ACGT]AGC
BpuAI 1167 GTCTTC GAAGAC(2/6)
BsaI 2142 GAGACC GGTCTC(1/5)
BsiEI 2126 CGGTCG CG[GA][CT]^CG
BsmI 1789 GAATGC GAATGC(1/-1)
BsrDI 944 GCAATG GCAATG(2/0)
BsrFI 2237 ACCGGC [GA]^CCGG[CT]
BstYI 71 AGATCC [GA]^GATC[CT]
Bsu36I 1362 CCTGAGG CC^T[ACGT]AGG
CelII 330 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2237 ACCGGC [GA]^CCGG[CT]
Eco47III 662 AGCGCT AGC^GCT
Eco81I 1362 CCTGAGG CC^T[ACGT]AGG
FspI 566 TGCGCA TGC^GCA
MaeI 1519 CTAG C^TAG
MflI 71 AGATCC [GA]^GATC[CT]
MluI 507 ACGCGT A^CGCGT
NspI 1783 GCATGC [GA]CATG^[CT]
PaeR7I 263 CTCGAG C^TCGAG
SacI 1659 GAGCTC GAGCT^C
SmlI 263 CTCGAG C^T[CT][GA]AG
SphI 1783 GCATGC GCATG^C
XhoI 263 CTCGAG C^TCGAG
XhoII 71 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwI 72 GATCC GGATC(4/5)
1269 GGATC
ApaI 1373 GGGCCC GGGCC^C
1600 GGGCCC
BalI 1470 TGGCCA TGG^CCA
1985 TGGCCA
BbvCI 439 GCTGAGG CCTCAGC(-5/-2)
1264 GCTGAGG
BsmAI 1938 GAGAC GTCTC(1/5)
2142 GAGAC
DpnII 72 GATC ^GATC
1270 GATC
DraIII 688 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1413 CACGCTGTG
DsaI 297 CCGCGG C^C[GA][CT]GG
345 CCGCGG
EarI 1191 CTCTTC CTCTTC(1/4)
1248 GAAGAG
Ksp632I 1191 CTCTTC CTCTTC(1/4)
1248 GAAGAG
KspI 297 CCGCGG CCGC^GG
345 CCGCGG
MaeII 1130 ACGT A^CGT
1289 ACGT
MboI 72 GATC ^GATC
1270 GATC
MluNI 1470 TGGCCA TGG^CCA
1985 TGGCCA
MscI 1470 TGGCCA TGG^CCA
1985 TGGCCA
NdeII 72 GATC ^GATC
1270 GATC
PflMI 92 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1872 CCAGTGCTTGG
PleI 766 GACTC GAGTC(4/5)
1120 GACTC
PpuMI 127 GGGTCCT [GA]G^G[AT]CC[CT]
1239 GGGTCCT
SacII 297 CCGCGG CCGC^GG
345 CCGCGG
Sau3AI 72 GATC ^GATC
1270 GATC
Tsp509I 59 AATT ^AATT
1429 AATT
Van91I 92 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1872 CCAGTGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrBI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeIII MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIII NlaIV
PstI PvuII RsaI Sau96I ScrFI
SfaNI SfcI SfoI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
XcmI