Restriction map for
pF1KB3027
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccIII AclI AflII AscI
AseI AsnI AspI AviII AvrII
BalI BbrPI BbvCI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BsrGI BssHII BstBI BstEII BstPI
CelII ClaI CpoI DraI DrdI
EagI EclXI Eco52I EcoO65I EcoRI
FspI HincII HindII HpaI MamI
MfeI MluI MluNI MroI MscI
MunI NdeI NheI NotI NruI
NspV PacI PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 1983 GGTACC G^GTACC
AcsI 2064 GAATTT [GA]^AATT[CT]
AgeI 596 ACCGGT A^CCGGT
AhdI 2998 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 898 GTGCAC G^TGCAC
Aor51HI 3028 AGCGCT AGC^GCT
ApaLI 898 GTGCAC G^TGCAC
ApoI 2064 GAATTT [GA]^AATT[CT]
Asp718I 1983 GGTACC G^GTACC
AspEI 2998 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1067 GGATCC G^GATCC
BciVI 3123 GGATAC GTATCC(6/5)
BglII 728 AGATCT A^GATCT
BsgI 1618 CTGCAC GTGCAG(16/14)
BsmI 2388 GAATGC GAATGC(1/-1)
Bst1107I 2959 GTATAC GTA^TAC
BstZ17I 2959 GTATAC GTA^TAC
DraIII 2417 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2998 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3028 AGCGCT AGC^GCT
EcoRV 676 GATATC GAT^ATC
EcoT22I 2239 ATGCAT ATGCA^T
FseI 2725 GGCCGGCC GGCCGG^CC
HindIII 2464 AAGCTT A^AGCTT
KpnI 1983 GGTACC GGTAC^C
KspI 3168 CCGCGG CCGC^GG
MseI 1751 TTAA T^TAA
NaeI 2726 GCCGGC GCC^GGC
NgoMIV 2726 GCCGGC G^CCGGC
NsiI 2239 ATGCAT ATGCA^T
PaeR7I 2517 CTCGAG C^TCGAG
PinAI 596 ACCGGT A^CCGGT
SacII 3168 CCGCGG CCGC^GG
SfiI 2720 GGCCGGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2237 GCATGC GCATG^C
TfiI 1726 GATTC G^A[AT]TC
Tru9I 1751 TTAA T^TAA
XhoI 2517 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 91 GTCTAC GT^[AC][GT]AC
2959 GTATAC
AccII 316 CGCG CG^CG
3169 CGCG
AflIII 881 ACATGT A^C[GA][CT]GT
2630 ACATGT
Asp700I 21 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2946 GAAGAGTTTC
BclI 2432 TGATCA T^GATCA
3305 TGATCA
BsaWI 596 ACCGGT [AT]^CCGG[AT]
3041 ACCGGA
BspHI 680 TCATGA T^CATGA
2804 TCATGA
BspLU11I 881 ACATGT A^CATGT
2630 ACATGT
BstUI 316 CGCG CG^CG
3169 CGCG
BstXI 1135 CCAGCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1242 CCAGCAGGCTGG
EcoNI 3510 CCTAGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3572 CCTACCACAGG
FbaI 2432 TGATCA T^GATCA
3305 TGATCA
MaeII 1671 ACGT A^CGT
2490 ACGT
MvnI 316 CGCG CG^CG
3169 CGCG
RcaI 680 TCATGA T^CATGA
2804 TCATGA
SapI 1596 GAAGAGC GCTCTTC(1/4)
2302 GAAGAGC
StuI 2616 AGGCCT AGG^CCT
3146 AGGCCT
Tsp509I 1817 AATT ^AATT
2065 AATT
XmnI 21 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2946 GAAGAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII