Restriction map for
pF1KB3028
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# NO CUT ENZYMES ( 88 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AgeI AhdI Alw44I ApaLI
ApoI AscI AspEI AviII AvrII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HindIII
KasI KspI MamI MfeI MluI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SfiI SfoI SfuI SnaBI SpeI
SspI SwaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 676 GGTACC G^GTACC
AccI 920 GTCGAC GT^[AC][GT]AC
AccII 1406 CGCG CG^CG
AflIII 1229 ACATGT A^C[GA][CT]GT
Aor51HI 3368 AGCGCT AGC^GCT
AseI 1471 ATTAAT AT^TAAT
AsnI 1471 ATTAAT AT^TAAT
Asp700I 1093 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 676 GGTACC G^GTACC
AspI 2530 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BsmBI 2730 GAGACG CGTCTC(1/5)
Bsp1407I 2465 TGTACA T^GTACA
BspHI 1452 TCATGA T^CATGA
BspLU11I 1229 ACATGT A^CATGT
BsrBI 2833 GAGCGG CCGCTC(-3/-3)
BsrGI 2465 TGTACA T^GTACA
BstEII 78 GGTGACC G^GT[ACGT]ACC
BstPI 78 GGTGACC G^GT[ACGT]ACC
BstUI 1406 CGCG CG^CG
DraIII 2326 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3368 AGCGCT AGC^GCT
EcoO65I 78 GGTGACC G^GT[ACGT]ACC
HpaI 639 GTTAAC GTT^AAC
KpnI 676 GGTACC GGTAC^C
MvnI 1406 CGCG CG^CG
NaeI 1585 GCCGGC GCC^GGC
NgoMIV 1585 GCCGGC G^CCGGC
PflFI 2530 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 976 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1471 ATTAAT AT^TAAT
RcaI 1452 TCATGA T^CATGA
SalI 920 GTCGAC G^TCGAC
SbfI 2433 CCTGCAGG CCTGCA^GG
ScaI 2951 AGTACT AGT^ACT
SexAI 774 ACCTGGT A^CC[AT]GGT
SgrAI 2501 CACCGGCG C[GA]^CCGG[CT]G
SmaI 2576 CCCGGG CCC^GGG
SmlI 2133 CTTGAG C^T[CT][GA]AG
SphI 3377 GCATGC GCATG^C
Sse8387I 2433 CCTGCAGG CCTGCA^GG
SspBI 2465 TGTACA T^GTACA
StuI 2632 AGGCCT AGG^CCT
Tsp509I 2918 AATT ^AATT
Tth111I 2530 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 927 TCTAGA T^CTAGA
XmaCI 2576 CCCGGG C^CCGGG
XmaI 2576 CCCGGG C^CCGGG
XmnI 1093 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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BfaI 928 CTAG C^TAG
3091 CTAG
BsaI 568 GAGACC GGTCTC(1/5)
2675 GAGACC
BsrDI 1003 CATTGC GCAATG(2/0)
1916 GCAATG
BssSI 3306 CACGAG CACGAG(-5/-1)
2329 CTCGTG
BstXI 227 CCATTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1784 CCAATGGCTTGG
EcoNI 811 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2608 CCTGCCACAGG
HgaI 2472 GACGC GACGC(5/10)
918 GCGTC
MaeI 928 CTAG C^TAG
3091 CTAG
MseI 640 TTAA T^TAA
1472 TTAA
PstI 2434 CTGCAG CTGCA^G
3233 CTGCAG
SapI 3097 GAAGAGC GCTCTTC(1/4)
1096 GCTCTTC
Tru9I 640 TTAA T^TAA
1472 TTAA
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BamHI BanI BanII BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII