Restriction map for
pF1KB3037
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BcgI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaHI BseAI BsiEI BsiHKAI BsiWI
BsoBI BspDI BspEI BspLU11I BsrBI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraII DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRV FseI FspI
HaeII Hin1I HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AlwNI 1022 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1441 CCTAAGG CC^T[ACGT]AGG
AspI 1307 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 486 TGGCCA TGG^CCA
BbvCI 3271 GCTGAGG CCTCAGC(-5/-2)
BcnI 2076 CCCGG CC^[GC]GG
Bsp1407I 1095 TGTACA T^GTACA
BsrDI 2349 CATTGC GCAATG(2/0)
BsrGI 1095 TGTACA T^GTACA
BstXI 222 CCATGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1441 CCTAAGG CC^T[ACGT]AGG
CfoI 2107 GCGC GCG^C
DrdI 2334 GACAGCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 486 TGGCCA [CT]^GGCC[GA]
Eco81I 1441 CCTAAGG CC^T[ACGT]AGG
EcoT14I 3029 CCATGG C^C[AT][AT]GG
EcoT22I 756 ATGCAT ATGCA^T
FauI 2528 CCCGC CCCGC(4/6)
HgaI 3285 GACGC GACGC(5/10)
HhaI 2107 GCGC GCG^C
HinP1I 2107 GCGC G^CGC
HindIII 3427 AAGCTT A^AGCTT
MaeII 2497 ACGT A^CGT
MluNI 486 TGGCCA TGG^CCA
MscI 486 TGGCCA TGG^CCA
NciI 2076 CCCGG CC^[GC]GG
NcoI 3029 CCATGG C^CATGG
NsiI 756 ATGCAT ATGCA^T
NspI 1359 ACATGC [GA]CATG^[CT]
PflFI 1307 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 3092 CAGCTG CAG^CTG
SexAI 847 ACCAGGT A^CC[AT]GGT
SpeI 1812 ACTAGT A^CTAGT
SspBI 1095 TGTACA T^GTACA
StuI 266 AGGCCT AGG^CCT
StyI 3029 CCATGG C^C[AT][AT]GG
Tth111I 1307 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2978 CCATGTATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AgeI 1639 ACCGGT A^CCGGT
3260 ACCGGT
BciVI 579 GGATAC GTATCC(6/5)
2478 GGATAC
BsaBI 193 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2731 GATACTGATC
BsaWI 1639 ACCGGT [AT]^CCGG[AT]
3260 ACCGGT
BseRI 3004 GAGGAG GAGGAG(10/8)
3274 GAGGAG
BsmBI 2963 GAGACG CGTCTC(1/5)
3283 GAGACG
BsmI 1819 GAATGC GAATGC(1/-1)
2103 GAATGC
BspMI 100 ACCTGC ACCTGC(4/8)
3354 ACCTGC
Cac8I 3090 GCCAGC GC[ACGT]^[ACGT]GC
3403 GCAAGC
DraI 726 TTTAAA TTT^AAA
1617 TTTAAA
EcoRI 883 GAATTC G^AATTC
2518 GAATTC
HincII 1635 GTTGAC GT[CT]^[GA]AC
2569 GTCAAC
HindII 1635 GTTGAC GT[CT]^[GA]AC
2569 GTCAAC
MamI 193 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2731 GATACTGATC
PflMI 514 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
970 CCAGTGTTTGG
PinAI 1639 ACCGGT A^CCGGT
3260 ACCGGT
PstI 2306 CTGCAG CTGCA^G
2427 CTGCAG
ScaI 28 AGTACT AGT^ACT
2993 AGTACT
SmlI 1660 CTTGAG C^T[CT][GA]AG
1805 CTCAAG
SspI 2556 AATATT AAT^ATT
3393 AATATT
Tsp45I 1625 GTCAC ^GT[GC]AC
2332 GTGAC
Van91I 514 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
970 CCAGTGTTTGG
XbaI 912 TCTAGA T^CTAGA
2436 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BbsI BbvI
BclI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cfr10I Cfr13I DdeI DpnII
DsaI EarI EcoRII FbaI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII