Restriction map for
pF1KB3043
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# NO CUT ENZYMES ( 68 enzymes) #
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AatII Acc65I AclI AflII AgeI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BciVI BfrI BlnI
BsaBI BsgI BsiWI BsmBI BspDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EcoT22I FseI
FspI HgaI HindIII KpnI KspI
MamI MfeI MluI MunI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflMI PinAI PmeI
PshBI Psp1406I RsrII SacI SacII
SalI SfuI SgrAI SnaBI SpeI
SphI SspI StuI SwaI Van91I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 587 TCCGGA T^CCGGA
AhdI 33 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 325 GTGCAC G^TGCAC
ApaLI 325 GTGCAC G^TGCAC
AspEI 33 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1417 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1613 TGGCCA TGG^CCA
BfaI 757 CTAG C^TAG
BseAI 587 TCCGGA T^CCGGA
BsmI 741 GAATGC GAATGC(1/-1)
Bsp1407I 3380 TGTACA T^GTACA
BspEI 587 TCCGGA T^CCGGA
BspHI 2357 TCATGA T^CATGA
BspLU11I 257 ACATGT A^CATGT
BspMI 234 GCAGGT ACCTGC(4/8)
BsrBI 3319 CCGCTC CCGCTC(-3/-3)
BsrFI 2757 GCCGGT [GA]^CCGG[CT]
BsrGI 3380 TGTACA T^GTACA
Cfr10I 2757 GCCGGT [GA]^CCGG[CT]
DraIII 1757 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2046 CGGCCG C^GGCCG
Eam1105I 33 GACCTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2046 CGGCCG C^GGCCG
Eco52I 2046 CGGCCG C^GGCCG
EcoNI 1484 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1274 GATATC GAT^ATC
HincII 21 GTTAAC GT[CT]^[GA]AC
HindII 21 GTTAAC GT[CT]^[GA]AC
HpaI 21 GTTAAC GTT^AAC
MaeI 757 CTAG C^TAG
MluNI 1613 TGGCCA TGG^CCA
MroI 587 TCCGGA T^CCGGA
MscI 1613 TGGCCA TGG^CCA
NcoI 2793 CCATGG C^CATGG
NdeI 2943 CATATG CA^TATG
PaeR7I 2982 CTCGAG C^TCGAG
PflFI 1417 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 1001 CGATCG CGAT^CG
RcaI 2357 TCATGA T^CATGA
SbfI 231 CCTGCAGG CCTGCA^GG
SexAI 1418 ACCTGGT A^CC[AT]GGT
SfiI 1614 GGCCATTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 231 CCTGCAGG CCTGCA^GG
SspBI 3380 TGTACA T^GTACA
Tth111I 1417 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2982 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 39 enzymes) #
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AccII 2062 CGCG CG^CG
3375 CGCG
AcyI 1360 GGCGCC G[GA]^CG[CT]C
2528 GGCGCC
AocI 817 CCTGAGG CC^T[ACGT]AGG
1673 CCTCAGG
BamHI 127 GGATCC G^GATCC
379 GGATCC
BbeI 1360 GGCGCC GGCGC^C
2528 GGCGCC
BbrPI 931 CACGTG CAC^GTG
1822 CACGTG
BbsI 963 GAAGAC GAAGAC(2/6)
2068 GTCTTC
BbvCI 298 CCTCAGC CCTCAGC(-5/-2)
2337 CCTCAGC
BcgI 1349 GCACCCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3332 GCAGCTCTGTCG
BclI 927 TGATCA T^GATCA
1917 TGATCA
BglI 1615 GCCATTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2919 GCCCGTCCGGC
BglII 992 AGATCT A^GATCT
3212 AGATCT
BlpI 1095 GCTGAGC GC^T[ACGT]AGC
3034 GCTGAGC
Bpu1102I 1095 GCTGAGC GC^T[ACGT]AGC
3034 GCTGAGC
BpuAI 963 GAAGAC GAAGAC(2/6)
2068 GTCTTC
BsaHI 1360 GGCGCC G[GA]^CG[CT]C
2528 GGCGCC
BsaWI 587 TCCGGA [AT]^CCGG[AT]
2483 ACCGGA
BsiEI 1001 CGATCG CG[GA][CT]^CG
2046 CGGCCG
BssSI 923 CTCGTG CACGAG(-5/-1)
2374 CTCGTG
BstAPI 1821 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2582 GCACCAGCTGC
BstUI 2062 CGCG CG^CG
3375 CGCG
Bsu36I 817 CCTGAGG CC^T[ACGT]AGG
1673 CCTCAGG
CelII 1095 GCTGAGC GC^T[ACGT]AGC
3034 GCTGAGC
Eco81I 817 CCTGAGG CC^T[ACGT]AGG
1673 CCTCAGG
FbaI 927 TGATCA T^GATCA
1917 TGATCA
Hin1I 1360 GGCGCC G[GA]^CG[CT]C
2528 GGCGCC
KasI 1360 GGCGCC G^GCGCC
2528 GGCGCC
MvnI 2062 CGCG CG^CG
3375 CGCG
NarI 1360 GGCGCC GG^CGCC
2528 GGCGCC
PmaCI 931 CACGTG CAC^GTG
1822 CACGTG
PmlI 931 CACGTG CAC^GTG
1822 CACGTG
PshAI 2103 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2445 GACACAGGTC
PstI 232 CTGCAG CTGCA^G
2589 CTGCAG
SapI 1974 GAAGAGC GCTCTTC(1/4)
2577 GAAGAGC
ScaI 572 AGTACT AGT^ACT
1848 AGTACT
SfoI 1360 GGCGCC GGC^GCC
2528 GGCGCC
SmaI 2390 CCCGGG CCC^GGG
3438 CCCGGG
XmaCI 2390 CCCGGG C^CCGGG
3438 CCCGGG
XmaI 2390 CCCGGG C^CCGGG
3438 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco47III
EcoO109I EcoO65I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII